Circovirus-like genome CB-A

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GII6|C6GII6_9VIRU Uncharacterized protein OS=Circovirus-like genome CB-A OX=642256 PE=4 SV=1
MM1 pKa = 7.17SRR3 pKa = 11.84SRR5 pKa = 11.84NWCFTYY11 pKa = 10.93NNYY14 pKa = 10.46DD15 pKa = 3.28SSPRR19 pKa = 11.84IQKK22 pKa = 9.5CMKK25 pKa = 8.58YY26 pKa = 8.75LTYY29 pKa = 10.05GYY31 pKa = 10.22EE32 pKa = 4.13VGEE35 pKa = 4.38SGTPHH40 pKa = 6.23LQGFVIFKK48 pKa = 10.37NAVAKK53 pKa = 10.07PSQYY57 pKa = 10.52FKK59 pKa = 10.39PAYY62 pKa = 9.51HH63 pKa = 6.33FEE65 pKa = 4.33KK66 pKa = 10.88ARR68 pKa = 11.84GTPQQALEE76 pKa = 4.09YY77 pKa = 9.06CQKK80 pKa = 10.96DD81 pKa = 3.74GNFKK85 pKa = 10.9EE86 pKa = 5.01FGEE89 pKa = 4.41KK90 pKa = 10.06PKK92 pKa = 10.42TVADD96 pKa = 4.08GGAAGGEE103 pKa = 4.23ANKK106 pKa = 10.03RR107 pKa = 11.84RR108 pKa = 11.84YY109 pKa = 9.66EE110 pKa = 4.1EE111 pKa = 4.15AFSAAKK117 pKa = 9.47EE118 pKa = 3.99GRR120 pKa = 11.84MDD122 pKa = 6.65DD123 pKa = 3.7IPADD127 pKa = 3.17IYY129 pKa = 10.48IRR131 pKa = 11.84HH132 pKa = 6.06YY133 pKa = 10.24STLKK137 pKa = 10.52KK138 pKa = 10.1IRR140 pKa = 11.84FDD142 pKa = 3.67HH143 pKa = 7.29APPAQNNDD151 pKa = 2.93VLNNYY156 pKa = 7.25WVYY159 pKa = 11.03GPSGTGKK166 pKa = 9.65SKK168 pKa = 10.42SVRR171 pKa = 11.84EE172 pKa = 4.07FFGKK176 pKa = 9.85SLYY179 pKa = 10.56VKK181 pKa = 10.22NQNKK185 pKa = 8.09WFDD188 pKa = 3.68GYY190 pKa = 10.93EE191 pKa = 4.21GEE193 pKa = 5.11DD194 pKa = 3.53FVLIDD199 pKa = 4.53DD200 pKa = 4.5VHH202 pKa = 6.64PNWSGKK208 pKa = 8.88TILKK212 pKa = 9.42IWSDD216 pKa = 4.05HH217 pKa = 5.82YY218 pKa = 11.05PFSPEE223 pKa = 3.73TKK225 pKa = 9.5GGHH228 pKa = 5.51IKK230 pKa = 10.07MIRR233 pKa = 11.84PEE235 pKa = 4.47GIIVTSNYY243 pKa = 9.41TIEE246 pKa = 4.15EE247 pKa = 4.21MYY249 pKa = 10.47EE250 pKa = 4.0AEE252 pKa = 5.16EE253 pKa = 4.07DD254 pKa = 3.51RR255 pKa = 11.84QPIRR259 pKa = 11.84RR260 pKa = 11.84RR261 pKa = 11.84FKK263 pKa = 10.39VIKK266 pKa = 10.46SEE268 pKa = 3.89AGYY271 pKa = 10.7DD272 pKa = 3.83VIMKK276 pKa = 9.25EE277 pKa = 3.94YY278 pKa = 9.48NKK280 pKa = 10.19RR281 pKa = 11.84VAATKK286 pKa = 9.79ICSNTDD292 pKa = 2.73EE293 pKa = 4.6DD294 pKa = 4.71VVVAEE299 pKa = 5.19HH300 pKa = 6.42EE301 pKa = 4.23PCRR304 pKa = 11.84PATRR308 pKa = 11.84ASAPGDD314 pKa = 3.63HH315 pKa = 6.94EE316 pKa = 4.89LPPPATEE323 pKa = 3.89VQSIVVAVIEE333 pKa = 4.74SPPPPATTDD342 pKa = 3.12QQ343 pKa = 3.5

Molecular weight: 38.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GII6|C6GII6_9VIRU Uncharacterized protein OS=Circovirus-like genome CB-A OX=642256 PE=4 SV=1
MM1 pKa = 6.65YY2 pKa = 10.05RR3 pKa = 11.84YY4 pKa = 9.18YY5 pKa = 10.86KK6 pKa = 10.25KK7 pKa = 10.62KK8 pKa = 9.18NAVRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84ATLKK18 pKa = 10.4RR19 pKa = 11.84SFKK22 pKa = 10.33RR23 pKa = 11.84GFRR26 pKa = 11.84RR27 pKa = 11.84AYY29 pKa = 8.79TPKK32 pKa = 10.29KK33 pKa = 9.78SYY35 pKa = 10.4RR36 pKa = 11.84PYY38 pKa = 10.99RR39 pKa = 11.84KK40 pKa = 9.32FKK42 pKa = 10.88SVNPKK47 pKa = 10.07IKK49 pKa = 10.4VPNIKK54 pKa = 10.22VPIAFSSTVALSTSADD70 pKa = 3.57KK71 pKa = 11.04IFTPEE76 pKa = 3.64PTLKK80 pKa = 10.87DD81 pKa = 3.61NEE83 pKa = 4.24VHH85 pKa = 6.26DD86 pKa = 4.43TYY88 pKa = 11.36TRR90 pKa = 11.84VYY92 pKa = 9.56NKK94 pKa = 9.88HH95 pKa = 5.71KK96 pKa = 10.53VVSIVNYY103 pKa = 7.5VQPHH107 pKa = 5.39QYY109 pKa = 10.52SSYY112 pKa = 10.26YY113 pKa = 9.55YY114 pKa = 9.42NLVGNGEE121 pKa = 4.05PLKK124 pKa = 10.46YY125 pKa = 10.04VVYY128 pKa = 10.67KK129 pKa = 9.61ITPDD133 pKa = 3.42TLGDD137 pKa = 3.51EE138 pKa = 4.61SKK140 pKa = 9.27PTNFRR145 pKa = 11.84EE146 pKa = 4.1AMAMPGARR154 pKa = 11.84AYY156 pKa = 11.18SLTRR160 pKa = 11.84SSKK163 pKa = 10.14RR164 pKa = 11.84MRR166 pKa = 11.84IAQMGVVNTYY176 pKa = 10.82LKK178 pKa = 10.86GSEE181 pKa = 4.15FVDD184 pKa = 3.59ANLTNVRR191 pKa = 11.84QSRR194 pKa = 11.84RR195 pKa = 11.84ATYY198 pKa = 10.18IPTNTLEE205 pKa = 5.46SDD207 pKa = 3.66LPWYY211 pKa = 10.47FGFRR215 pKa = 11.84IWFPQLTGGTLNLPPPSFEE234 pKa = 4.33VISVLNVSYY243 pKa = 11.11IMPTNASLTTIKK255 pKa = 10.75KK256 pKa = 10.35EE257 pKa = 4.72DD258 pKa = 3.58DD259 pKa = 3.7TLPDD263 pKa = 3.13NMFNLNCNSDD273 pKa = 4.23CIFF276 pKa = 4.13

Molecular weight: 31.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

619

276

343

309.5

35.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.462 ± 0.853

1.131 ± 0.249

4.847 ± 0.529

5.977 ± 1.668

4.523 ± 0.115

5.493 ± 1.148

1.939 ± 0.523

5.331 ± 0.381

8.239 ± 0.058

4.362 ± 1.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.268

5.816 ± 0.878

7.27 ± 0.014

2.585 ± 0.475

6.139 ± 0.68

6.947 ± 0.629

6.462 ± 1.149

6.785 ± 0.506

1.131 ± 0.249

6.462 ± 0.482
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski