Diplodia scrobiculata RNA virus 1

Taxonomy: Viruses; Riboviria; dsRNA viruses; unclassified dsRNA viruses

Average proteome isoelectric point is 8.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2CPV7|D2CPV7_9VIRU RNA-directed RNA polymerase OS=Diplodia scrobiculata RNA virus 1 OX=459770 GN=RdRp PE=4 SV=1
MM1 pKa = 7.63SGRR4 pKa = 11.84LLAHH8 pKa = 7.08KK9 pKa = 10.28GYY11 pKa = 10.7VLQDD15 pKa = 3.33FAAIRR20 pKa = 11.84KK21 pKa = 8.45FYY23 pKa = 10.76NLQRR27 pKa = 11.84PPRR30 pKa = 11.84DD31 pKa = 3.39TVEE34 pKa = 3.61AAAAYY39 pKa = 9.9LFLEE43 pKa = 4.71PCPAEE48 pKa = 3.68AWLFLPPDD56 pKa = 4.34LLWDD60 pKa = 3.77DD61 pKa = 4.11TLPFPVSEE69 pKa = 4.05PRR71 pKa = 11.84FLRR74 pKa = 11.84RR75 pKa = 11.84DD76 pKa = 3.11SHH78 pKa = 4.95TTRR81 pKa = 11.84SSLKK85 pKa = 10.27LDD87 pKa = 3.48ASLLTKK93 pKa = 10.71LEE95 pKa = 3.92AAYY98 pKa = 8.44PPPSGRR104 pKa = 11.84PGGKK108 pKa = 8.81ILADD112 pKa = 3.2WRR114 pKa = 11.84LVFAYY119 pKa = 10.31LRR121 pKa = 11.84GAAPTRR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84EE130 pKa = 3.97LAMSTLAMLAATDD143 pKa = 4.43ADD145 pKa = 3.71WGSIAAISLVLLPYY159 pKa = 10.43FSTLGGVYY167 pKa = 10.02AAEE170 pKa = 4.21AMLAILPDD178 pKa = 3.7VLPPLVEE185 pKa = 3.87YY186 pKa = 10.97AKK188 pKa = 10.57LCKK191 pKa = 9.89ALHH194 pKa = 5.37QGLRR198 pKa = 11.84LTYY201 pKa = 8.63TFPRR205 pKa = 11.84PDD207 pKa = 2.65GCYY210 pKa = 10.32ARR212 pKa = 11.84LSQEE216 pKa = 4.1EE217 pKa = 4.25VRR219 pKa = 11.84QLTGLDD225 pKa = 3.38VLPGRR230 pKa = 11.84NEE232 pKa = 3.82VFAVSHH238 pKa = 5.39VGGMFMRR245 pKa = 11.84ASYY248 pKa = 11.08GSLPTTLLRR257 pKa = 11.84SADD260 pKa = 3.76GTPHH264 pKa = 6.75YY265 pKa = 9.96DD266 pKa = 3.25RR267 pKa = 11.84PSFDD271 pKa = 3.03QEE273 pKa = 3.72MRR275 pKa = 11.84EE276 pKa = 4.46CIAEE280 pKa = 4.17ALQSILPNKK289 pKa = 8.52LQPNTFSQWYY299 pKa = 8.85ARR301 pKa = 11.84RR302 pKa = 11.84EE303 pKa = 3.65FWAASGGAPGAKK315 pKa = 9.15MSWLRR320 pKa = 11.84QDD322 pKa = 4.68SEE324 pKa = 4.89SPDD327 pKa = 3.07AKK329 pKa = 10.49IEE331 pKa = 4.03VEE333 pKa = 3.9RR334 pKa = 11.84VRR336 pKa = 11.84LNKK339 pKa = 9.85RR340 pKa = 11.84AALLLVPEE348 pKa = 5.0KK349 pKa = 9.62YY350 pKa = 9.84HH351 pKa = 6.34RR352 pKa = 11.84RR353 pKa = 11.84HH354 pKa = 5.73FADD357 pKa = 4.16PSSAVLYY364 pKa = 10.81SKK366 pKa = 10.61AAPKK370 pKa = 10.37FEE372 pKa = 4.18NGKK375 pKa = 9.81RR376 pKa = 11.84RR377 pKa = 11.84IIWNTSLTHH386 pKa = 5.67YY387 pKa = 9.35VAQGFLLDD395 pKa = 3.73LVEE398 pKa = 3.98PRR400 pKa = 11.84FRR402 pKa = 11.84QGTWYY407 pKa = 10.42SAANSAPHH415 pKa = 5.49RR416 pKa = 11.84TARR419 pKa = 11.84SIARR423 pKa = 11.84FNEE426 pKa = 3.5LHH428 pKa = 6.0GAIGFMWDD436 pKa = 3.33YY437 pKa = 11.89ADD439 pKa = 5.11FNINHH444 pKa = 5.86SRR446 pKa = 11.84DD447 pKa = 3.51AQIMLFSVLADD458 pKa = 4.8LISEE462 pKa = 4.59RR463 pKa = 11.84LPRR466 pKa = 11.84DD467 pKa = 3.6DD468 pKa = 5.07PATAEE473 pKa = 3.99CRR475 pKa = 11.84ADD477 pKa = 3.55VVRR480 pKa = 11.84ITSWVNTAKK489 pKa = 10.76KK490 pKa = 10.29NFFMHH495 pKa = 7.3DD496 pKa = 3.42SEE498 pKa = 4.44TGYY501 pKa = 9.99VQEE504 pKa = 4.88AVRR507 pKa = 11.84SLASGEE513 pKa = 4.11RR514 pKa = 11.84ATSFVNTVLSRR525 pKa = 11.84AYY527 pKa = 10.42RR528 pKa = 11.84LQHH531 pKa = 6.32DD532 pKa = 3.68RR533 pKa = 11.84ASRR536 pKa = 11.84RR537 pKa = 11.84FFGRR541 pKa = 11.84QLLLPRR547 pKa = 11.84SDD549 pKa = 3.62HH550 pKa = 6.76QGDD553 pKa = 3.9DD554 pKa = 3.3VFATVASMPDD564 pKa = 3.05ATLAGCMFLLTGYY577 pKa = 10.47AGQLFKK583 pKa = 10.08ITCNHH588 pKa = 5.64TPSGEE593 pKa = 4.24FLRR596 pKa = 11.84QFYY599 pKa = 10.52GMHH602 pKa = 7.11GIAGYY607 pKa = 8.73PIRR610 pKa = 11.84SAMGLFSGEE619 pKa = 4.21YY620 pKa = 10.5FEE622 pKa = 6.34DD623 pKa = 4.49LPPDD627 pKa = 3.87PPSRR631 pKa = 11.84VSATFMQFSKK641 pKa = 10.74VAARR645 pKa = 11.84GGAFEE650 pKa = 4.19PHH652 pKa = 7.59LITLLSQRR660 pKa = 11.84NASLAYY666 pKa = 9.33SDD668 pKa = 4.63KK669 pKa = 10.9NGSVHH674 pKa = 7.05HH675 pKa = 5.64VTCPPALSITPRR687 pKa = 11.84SLGGLGGVTTRR698 pKa = 11.84RR699 pKa = 11.84YY700 pKa = 8.16QLKK703 pKa = 10.66GNVIRR708 pKa = 11.84DD709 pKa = 3.47AAATDD714 pKa = 3.78WTWVPRR720 pKa = 11.84SQLASPTGQTKK731 pKa = 9.76RR732 pKa = 11.84RR733 pKa = 11.84ALAIPSGEE741 pKa = 4.16GKK743 pKa = 6.8TTLARR748 pKa = 11.84MYY750 pKa = 10.04PSFFVDD756 pKa = 3.82HH757 pKa = 7.43DD758 pKa = 4.24ALLSVWDD765 pKa = 4.37IPPSPRR771 pKa = 11.84RR772 pKa = 11.84AARR775 pKa = 11.84TAYY778 pKa = 10.22CKK780 pKa = 10.21RR781 pKa = 11.84QSLLCPVDD789 pKa = 4.24KK790 pKa = 11.19VLLTWGPTTVDD801 pKa = 2.89SSFEE805 pKa = 3.93FLGSIMLQTPTGTRR819 pKa = 11.84ANAEE823 pKa = 3.74NRR825 pKa = 11.84RR826 pKa = 11.84YY827 pKa = 9.51LASVSQGKK835 pKa = 9.22RR836 pKa = 11.84VTVRR840 pKa = 11.84SQTQLPHH847 pKa = 6.62AALALASQRR856 pKa = 11.84VSTVAFEE863 pKa = 4.57SDD865 pKa = 3.09IPPPVVQMPEE875 pKa = 4.09LPVAPMVAKK884 pKa = 10.63AGLVDD889 pKa = 3.95TQTLATLQAHH899 pKa = 7.29RR900 pKa = 11.84LQEE903 pKa = 4.12VATQAVASSSFGGAFPRR920 pKa = 11.84ASLSASFARR929 pKa = 11.84YY930 pKa = 9.5ARR932 pKa = 11.84DD933 pKa = 2.76LDD935 pKa = 3.95AFVKK939 pKa = 10.17AVKK942 pKa = 9.76PRR944 pKa = 11.84ARR946 pKa = 11.84TLEE949 pKa = 4.11PAASSPQLAYY959 pKa = 10.54QRR961 pKa = 11.84ARR963 pKa = 11.84PIWDD967 pKa = 5.51AILQPNSDD975 pKa = 3.38PSVPYY980 pKa = 10.07DD981 pKa = 3.7HH982 pKa = 7.7PLPLPATVYY991 pKa = 10.85GDD993 pKa = 3.94YY994 pKa = 10.73IRR996 pKa = 11.84IMLDD1000 pKa = 2.71AGFSLGEE1007 pKa = 4.03VFLAAVDD1014 pKa = 4.05DD1015 pKa = 4.08QSPVCYY1021 pKa = 9.69HH1022 pKa = 6.52GLAGRR1027 pKa = 11.84IFALMQRR1034 pKa = 11.84RR1035 pKa = 11.84RR1036 pKa = 11.84LDD1038 pKa = 3.38VKK1040 pKa = 10.71AYY1042 pKa = 10.08RR1043 pKa = 11.84ATAWLFSAITPATPPEE1059 pKa = 4.12QSDD1062 pKa = 3.47LLARR1066 pKa = 11.84YY1067 pKa = 9.24ILGRR1071 pKa = 11.84VDD1073 pKa = 4.69LPPVASDD1080 pKa = 4.82RR1081 pKa = 11.84PGTDD1085 pKa = 2.75LNSTVRR1091 pKa = 11.84LISLRR1096 pKa = 11.84HH1097 pKa = 5.47LDD1099 pKa = 3.08NWGRR1103 pKa = 11.84EE1104 pKa = 3.97LMPLLFF1110 pKa = 5.26

Molecular weight: 122.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2CPV7|D2CPV7_9VIRU RNA-directed RNA polymerase OS=Diplodia scrobiculata RNA virus 1 OX=459770 GN=RdRp PE=4 SV=1
MM1 pKa = 7.51GEE3 pKa = 3.9WTEE6 pKa = 4.23MFASNPDD13 pKa = 3.62LGIMEE18 pKa = 4.35QAYY21 pKa = 8.73MRR23 pKa = 11.84LKK25 pKa = 8.93TQSACDD31 pKa = 3.51QLWFVGSTDD40 pKa = 3.66QRR42 pKa = 11.84VVDD45 pKa = 4.21PNLRR49 pKa = 11.84PPSKK53 pKa = 9.78PQKK56 pKa = 8.6TQITDD61 pKa = 3.03SDD63 pKa = 3.72RR64 pKa = 11.84QKK66 pKa = 11.19EE67 pKa = 4.16EE68 pKa = 4.63EE69 pKa = 4.22EE70 pKa = 4.16LQMALALSVKK80 pKa = 10.24EE81 pKa = 4.4SGTEE85 pKa = 3.62PSAARR90 pKa = 11.84SNQPQAKK97 pKa = 9.6APQQSAPEE105 pKa = 4.16QEE107 pKa = 4.02QHH109 pKa = 5.42QAIASGTTAATVSRR123 pKa = 11.84VRR125 pKa = 11.84ALYY128 pKa = 10.48DD129 pKa = 3.41FTPSEE134 pKa = 4.3PGEE137 pKa = 3.76LAFRR141 pKa = 11.84KK142 pKa = 10.12GDD144 pKa = 3.59IIAVLEE150 pKa = 4.21SVYY153 pKa = 10.97KK154 pKa = 10.47DD155 pKa = 2.65WWKK158 pKa = 11.08GSLRR162 pKa = 11.84GQTGIFPLNYY172 pKa = 9.45VEE174 pKa = 5.74KK175 pKa = 10.57LQDD178 pKa = 3.42PTKK181 pKa = 10.91EE182 pKa = 3.95EE183 pKa = 4.43LEE185 pKa = 4.62RR186 pKa = 11.84EE187 pKa = 4.3AQMEE191 pKa = 4.3AEE193 pKa = 4.42VFAEE197 pKa = 3.96IKK199 pKa = 10.65NVEE202 pKa = 4.22KK203 pKa = 10.86LLALLSTSSSADD215 pKa = 3.01ARR217 pKa = 11.84DD218 pKa = 3.65NEE220 pKa = 4.94EE221 pKa = 3.78ITSLYY226 pKa = 9.76HH227 pKa = 4.95KK228 pKa = 8.91TVSIRR233 pKa = 11.84PKK235 pKa = 10.4LIEE238 pKa = 5.01LIGKK242 pKa = 8.96YY243 pKa = 7.22SQKK246 pKa = 10.1KK247 pKa = 9.33GKK249 pKa = 9.14WNLLRR254 pKa = 11.84EE255 pKa = 4.49FVCWPLIVFQMISRR269 pKa = 11.84SSTRR273 pKa = 11.84SSSRR277 pKa = 11.84HH278 pKa = 4.9AATTKK283 pKa = 10.18PSLKK287 pKa = 9.83PRR289 pKa = 11.84CRR291 pKa = 11.84NQRR294 pKa = 11.84SPATVVNPMATAPAPRR310 pKa = 11.84RR311 pKa = 11.84LRR313 pKa = 11.84VLRR316 pKa = 11.84TVAILLKK323 pKa = 10.64DD324 pKa = 3.55LLRR327 pKa = 11.84NSFTRR332 pKa = 11.84LLQPQTKK339 pKa = 8.7ATDD342 pKa = 2.95ILLRR346 pKa = 11.84MGLSPSRR353 pKa = 11.84WLSPHH358 pKa = 5.85KK359 pKa = 10.2HH360 pKa = 5.8LLLHH364 pKa = 6.54RR365 pKa = 11.84WLVLDD370 pKa = 3.56QRR372 pKa = 11.84MASSPTSRR380 pKa = 11.84SACLSVEE387 pKa = 4.76GPHH390 pKa = 6.6PWPFPRR396 pKa = 11.84KK397 pKa = 8.71WRR399 pKa = 11.84PTAQWTPGRR408 pKa = 11.84LRR410 pKa = 11.84ASKK413 pKa = 9.94IRR415 pKa = 11.84KK416 pKa = 8.57

Molecular weight: 47.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1526

416

1110

763.0

85.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.009 ± 1.313

0.983 ± 0.011

4.915 ± 0.662

4.522 ± 1.118

3.997 ± 0.806

5.177 ± 0.795

2.097 ± 0.21

3.277 ± 0.288

3.735 ± 1.394

11.14 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.163 ± 0.122

2.163 ± 0.122

7.471 ± 0.009

4.325 ± 0.852

8.519 ± 0.053

8.322 ± 0.654

5.963 ± 0.267

5.505 ± 0.353

1.9 ± 0.376

2.818 ± 0.696
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski