Clostridiales bacterium 1_7_47FAA

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6251 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5EIX6|C5EIX6_9FIRM Aspartate carbamoyltransferase OS=Clostridiales bacterium 1_7_47FAA OX=457421 GN=pyrB PE=3 SV=1
MM1 pKa = 7.24NFIDD5 pKa = 5.41RR6 pKa = 11.84AFKK9 pKa = 10.87QNLSGDD15 pKa = 4.09DD16 pKa = 3.98FLQAMADD23 pKa = 3.37IYY25 pKa = 11.1SEE27 pKa = 4.36PEE29 pKa = 3.76VYY31 pKa = 10.18RR32 pKa = 11.84ILNQYY37 pKa = 9.24PPFVADD43 pKa = 4.14VISIIDD49 pKa = 4.02YY50 pKa = 7.98DD51 pKa = 3.94TALQMDD57 pKa = 4.63GLDD60 pKa = 5.25DD61 pKa = 4.92IISGNLSSRR70 pKa = 11.84YY71 pKa = 10.1AEE73 pKa = 4.02ILNALKK79 pKa = 10.49QCGAEE84 pKa = 4.0QEE86 pKa = 4.08ADD88 pKa = 3.14ILKK91 pKa = 9.84HH92 pKa = 6.34AKK94 pKa = 9.11EE95 pKa = 4.96LYY97 pKa = 10.56DD98 pKa = 4.47SDD100 pKa = 3.59EE101 pKa = 4.48DD102 pKa = 4.82GYY104 pKa = 11.76NEE106 pKa = 5.03EE107 pKa = 4.19YY108 pKa = 11.11DD109 pKa = 3.76NLNSQIALHH118 pKa = 6.28NDD120 pKa = 3.25YY121 pKa = 11.37NGFWDD126 pKa = 3.74IVRR129 pKa = 11.84AYY131 pKa = 9.67IDD133 pKa = 3.81KK134 pKa = 10.66NLNLII139 pKa = 4.27

Molecular weight: 15.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5ERE5|C5ERE5_9FIRM Amino acid carrier protein OS=Clostridiales bacterium 1_7_47FAA OX=457421 GN=agcS PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.44KK9 pKa = 7.54KK10 pKa = 9.66HH11 pKa = 5.36RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 5.73GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.82TVGGRR28 pKa = 11.84KK29 pKa = 9.22VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.26GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6251

0

6251

1916964

30

4317

306.7

34.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.983 ± 0.035

1.628 ± 0.013

5.656 ± 0.025

6.855 ± 0.035

4.015 ± 0.023

8.219 ± 0.036

1.83 ± 0.013

6.969 ± 0.026

5.577 ± 0.028

9.037 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.53 ± 0.017

3.818 ± 0.018

3.714 ± 0.019

3.26 ± 0.018

4.987 ± 0.023

5.809 ± 0.023

5.186 ± 0.023

6.918 ± 0.03

1.027 ± 0.011

3.982 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski