Geobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Geobacteraceae; Geobacter; Geobacter uraniireducens

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5GBY5|A5GBY5_GEOUR TPR repeat-containing protein OS=Geobacter uraniireducens (strain Rf4) OX=351605 GN=Gura_0702 PE=4 SV=1
MM1 pKa = 7.69SIRR4 pKa = 11.84MTSAKK9 pKa = 9.64ILRR12 pKa = 11.84GRR14 pKa = 11.84ISFVATLLCMVLTQPLSSFAASVTLDD40 pKa = 3.56AADD43 pKa = 4.48DD44 pKa = 4.44VYY46 pKa = 11.07TDD48 pKa = 3.37SSRR51 pKa = 11.84IPTAGYY57 pKa = 6.23TTTDD61 pKa = 3.25MASVKK66 pKa = 10.06DD67 pKa = 4.05DD68 pKa = 3.74SMSKK72 pKa = 9.3IVNYY76 pKa = 10.17VGRR79 pKa = 11.84TPSISVLNGRR89 pKa = 11.84TVVKK93 pKa = 10.15FNLSGISGAINSASFKK109 pKa = 10.88FNVIDD114 pKa = 3.43IHH116 pKa = 5.77GTPILNLIKK125 pKa = 10.35VTDD128 pKa = 4.15NSWSEE133 pKa = 4.05QPVGASTFPTYY144 pKa = 10.81TDD146 pKa = 2.95TDD148 pKa = 4.63YY149 pKa = 10.34ITQNSTPYY157 pKa = 10.26SNQSVTTGWNNINVKK172 pKa = 10.5DD173 pKa = 4.0YY174 pKa = 10.8IQEE177 pKa = 4.39KK178 pKa = 9.93INAGSPYY185 pKa = 9.27VTIAMTGYY193 pKa = 9.09ATGATDD199 pKa = 4.6DD200 pKa = 5.29DD201 pKa = 5.02FDD203 pKa = 5.09FVSLNNGDD211 pKa = 4.2ALIPTLTVDD220 pKa = 3.44YY221 pKa = 8.69TPPPTVTGISPSSGPVAGGTSVTISGANFTSATAVTFGSTNATSYY266 pKa = 9.51TVNSATQITATSPAGSAGTVDD287 pKa = 3.17ITVTTAGGTSATGASDD303 pKa = 3.23QFTYY307 pKa = 10.41IAAPTVTSISPTSGPTDD324 pKa = 3.12GSTSVTITGTNFTGATTVTIGGAAATSVTVVNATTLTATTPSGTAGVRR372 pKa = 11.84DD373 pKa = 3.8VVVTTPGGTGTGTGLFTYY391 pKa = 9.97KK392 pKa = 10.5ASQTITFNSPGAQNFGTTPTLTAIASSGLTPTFTSSTTGVCTITSGGALTFATTGTCTINAGQAGDD458 pKa = 3.9STYY461 pKa = 11.23LAALPVSQSFSVAAVVPGAPTIGTATAGDD490 pKa = 3.9TQASVTFTAPASNGGASITGYY511 pKa = 8.18TATSNPGGVTGTCASSPCTVTGLTNGTAYY540 pKa = 9.71TFTVTATNSAGTGSASAASNSVTPAAAQTITFNNPGAQNFGIAPTLTATASSGLMVSFTSSTTGVCTVTAGGALTFVTAGTCTINADD627 pKa = 3.47QAGNGSFTAAPMVTRR642 pKa = 11.84SFAVNAVVPGAPTIGTATAGDD663 pKa = 3.94TQASITFSAPASNGGASITSYY684 pKa = 9.89TATSNPGGLTGTCASSPCTVTGLTNGTAYY713 pKa = 9.71TFTVTATNSAGTGSASAASNSVTPAAAQTITFNNPGAQNFGTSPTLTATATSSLTVTFTSSTTGVCTVTAGGALTFVTTGTCTINADD800 pKa = 3.47QAGNGSFLAATTVSRR815 pKa = 11.84SFTVIAVVPGAPTIGIATAGDD836 pKa = 3.76TQASVAFTAPVSNGGASITGYY857 pKa = 8.63TVTSNPGGLTSSGASSPITVTGLTNGTAYY886 pKa = 9.73TFTVTAHH893 pKa = 6.25NSAGTGSASSASNSVTPNPGPTVVNVAVPANGIYY927 pKa = 10.3KK928 pKa = 10.43AGSNLDD934 pKa = 3.79FTVTWDD940 pKa = 3.16SAATVTGTPRR950 pKa = 11.84IALLIGSAMVYY961 pKa = 8.82ATYY964 pKa = 10.66QSGSGTASTLFRR976 pKa = 11.84YY977 pKa = 7.35TVLPGQTDD985 pKa = 3.39TDD987 pKa = 5.02GITVGALSLNGGTIQNSSGTDD1008 pKa = 2.99ATLTLNSVASTVNVLVDD1025 pKa = 3.61TTAPTLSSIATSNPTHH1041 pKa = 6.87SGGTLTATANEE1052 pKa = 4.12NALGSWIAVSSSATAPTVAQVLAGADD1078 pKa = 3.57YY1079 pKa = 11.5GGVTVVAHH1087 pKa = 6.4GSGALSAGSAVSFSLSGLVAATSYY1111 pKa = 11.01DD1112 pKa = 3.33IYY1114 pKa = 11.2LAAQDD1119 pKa = 3.9AAGNPSAAVSSATLITTTTRR1139 pKa = 11.84VVTTSSDD1146 pKa = 3.4SGPGSLRR1153 pKa = 11.84QTIADD1158 pKa = 4.45ANPGDD1163 pKa = 4.82IILFDD1168 pKa = 4.7PSLSGQTVTIASPLVIAKK1186 pKa = 9.72DD1187 pKa = 3.76LSIGGYY1193 pKa = 7.5DD1194 pKa = 4.12ARR1196 pKa = 11.84PITINGGGTTRR1207 pKa = 11.84IFQVSGSTTFTLNYY1221 pKa = 8.15LTLTDD1226 pKa = 4.86GVATDD1231 pKa = 4.61GGAISDD1237 pKa = 4.05NVNATTFISLCTFSGNTATAAGGAISAAGTMSISDD1272 pKa = 3.59TLFAGNSAVQGGAIFNNNALSLVNVTLANNSANSGGGIYY1311 pKa = 10.33SSGGSTLVKK1320 pKa = 9.68NTTIAGNNATVQGGGIEE1337 pKa = 4.16IASGAVGFRR1346 pKa = 11.84NSIVAGNTSPSGPDD1360 pKa = 2.72ISGFATSLGYY1370 pKa = 10.94NLVKK1374 pKa = 9.99DD1375 pKa = 3.7TSGATFTVTTGDD1387 pKa = 3.54LTGQDD1392 pKa = 4.01PLLGPLADD1400 pKa = 3.62NGGPIKK1406 pKa = 10.12TMALLLGSPAIDD1418 pKa = 3.4SGACTGASATDD1429 pKa = 3.62QRR1431 pKa = 11.84SMPRR1435 pKa = 11.84PQNGLCDD1442 pKa = 3.27MGAYY1446 pKa = 9.27EE1447 pKa = 4.65RR1448 pKa = 11.84GVPAALTATGGTPQSAAIDD1467 pKa = 3.65AAFATPLKK1475 pKa = 10.96AKK1477 pKa = 9.25VTDD1480 pKa = 3.63SLGGVMEE1487 pKa = 5.42GISVTFAGPGSGADD1501 pKa = 3.13ITADD1505 pKa = 3.28GSVTTDD1511 pKa = 2.94AAGIASYY1518 pKa = 10.98GVTANGTAGAYY1529 pKa = 7.62TVTATVNALIANFNLTNDD1547 pKa = 4.18KK1548 pKa = 10.93ANQAITFNPPATATFGDD1565 pKa = 4.0APIALSATGGASGNPVIFSVASGPGSLNGATLTITGAGNIVVTASQAGNANYY1617 pKa = 9.97NAAPQVIRR1625 pKa = 11.84NIAIGKK1631 pKa = 8.53GAATIALGSLNATYY1645 pKa = 10.68DD1646 pKa = 3.61GTAKK1650 pKa = 10.63AVTATTTPAGLAVIVTYY1667 pKa = 10.45GGSSTPPTAAGSYY1680 pKa = 9.25PVAATIDD1687 pKa = 3.59DD1688 pKa = 3.95ANYY1691 pKa = 10.58SGTATGTLVIAYY1703 pKa = 8.37SATPPVLTISTLADD1717 pKa = 3.25GSVTNNATLNVSGQATSINGITSVTVNGAAVTLAADD1753 pKa = 4.04GTFSQAVTLQAGTNTVTTIATDD1775 pKa = 3.49NAGTTTTDD1783 pKa = 2.49SRR1785 pKa = 11.84TITLDD1790 pKa = 2.78TTAPVITITTPADD1803 pKa = 3.43NSTLAASSVTITGSVDD1819 pKa = 2.92KK1820 pKa = 10.79NATVQATANSGSPQSAAMTNNTFTVTLNLAGGSNTIVISATDD1862 pKa = 3.6LAGNSASVKK1871 pKa = 9.12RR1872 pKa = 11.84TIVSDD1877 pKa = 3.48TTKK1880 pKa = 9.41PTLAITNPSQDD1891 pKa = 2.88ITISEE1896 pKa = 4.25PALTISGTVTDD1907 pKa = 4.6ALTDD1911 pKa = 3.41VSVTITCDD1919 pKa = 2.83GKK1921 pKa = 9.18TYY1923 pKa = 10.05TPQVVDD1929 pKa = 4.49GAFQQQLTFVMAKK1942 pKa = 9.48QYY1944 pKa = 10.93AITVTATDD1952 pKa = 3.34QAGNSVTTQRR1962 pKa = 11.84NVIYY1966 pKa = 9.92ALSSLPSGDD1975 pKa = 3.15INGDD1979 pKa = 3.62GKK1981 pKa = 11.42VDD1983 pKa = 3.26IADD1986 pKa = 4.06ALLALQMAVGLITPTSAQLATGDD2009 pKa = 4.0VAPLSNGKK2017 pKa = 8.45PAPDD2021 pKa = 3.62GAIDD2025 pKa = 3.6IADD2028 pKa = 3.46AMLILEE2034 pKa = 4.79KK2035 pKa = 10.64AVGMLTWW2042 pKa = 4.01

Molecular weight: 199.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5G9W5|A5G9W5_GEOUR SAM-dependent methyltransferase OS=Geobacter uraniireducens (strain Rf4) OX=351605 GN=Gura_1435 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.33QPSNVSRR12 pKa = 11.84KK13 pKa = 7.52RR14 pKa = 11.84THH16 pKa = 6.03GFLVRR21 pKa = 11.84MSTKK25 pKa = 10.31NGRR28 pKa = 11.84LVIKK32 pKa = 10.39RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.01GRR39 pKa = 11.84KK40 pKa = 8.3NLAVSIASKK49 pKa = 10.89

Molecular weight: 5.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4255

0

4255

1447873

31

7157

340.3

37.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.292 ± 0.05

1.28 ± 0.026

5.252 ± 0.031

6.396 ± 0.06

4.11 ± 0.027

8.001 ± 0.044

1.993 ± 0.025

6.226 ± 0.034

5.416 ± 0.041

9.91 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.469 ± 0.019

3.738 ± 0.038

4.374 ± 0.028

3.041 ± 0.024

5.711 ± 0.046

5.953 ± 0.038

5.615 ± 0.065

7.225 ± 0.033

1.027 ± 0.016

2.97 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski