Escherichia phage PDX

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Vequintavirus; unclassified Vequintavirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1CLC8|A0A2P1CLC8_9CAUD Uncharacterized protein OS=Escherichia phage PDX OX=2099658 PE=4 SV=1
MM1 pKa = 6.22TTVNITTGYY10 pKa = 10.02NLSFDD15 pKa = 4.03VLTDD19 pKa = 3.53EE20 pKa = 4.61QKK22 pKa = 10.83EE23 pKa = 4.13KK24 pKa = 10.49AIEE27 pKa = 3.94MVRR30 pKa = 11.84EE31 pKa = 4.09RR32 pKa = 11.84EE33 pKa = 4.0EE34 pKa = 3.94HH35 pKa = 6.28SGDD38 pKa = 3.3NFFAEE43 pKa = 5.25SVIEE47 pKa = 4.49DD48 pKa = 3.78YY49 pKa = 10.9KK50 pKa = 10.75EE51 pKa = 4.47HH52 pKa = 7.03IFPEE56 pKa = 4.36YY57 pKa = 10.62GLEE60 pKa = 3.92DD61 pKa = 3.89TKK63 pKa = 10.54VHH65 pKa = 7.0WSGFWCQGDD74 pKa = 3.93GASISAEE81 pKa = 3.81NVDD84 pKa = 4.33LEE86 pKa = 4.22KK87 pKa = 10.55FLRR90 pKa = 11.84KK91 pKa = 9.54VKK93 pKa = 10.62SLTKK97 pKa = 10.0FRR99 pKa = 11.84SIRR102 pKa = 11.84HH103 pKa = 5.69LFGTMHH109 pKa = 7.17DD110 pKa = 4.54GEE112 pKa = 4.77LSASVEE118 pKa = 3.84RR119 pKa = 11.84DD120 pKa = 3.06PYY122 pKa = 11.18SRR124 pKa = 11.84YY125 pKa = 8.57SHH127 pKa = 6.73EE128 pKa = 4.33NTVSGYY134 pKa = 10.1IDD136 pKa = 3.98TTWLDD141 pKa = 3.34LTAKK145 pKa = 10.16QEE147 pKa = 4.51SKK149 pKa = 11.03VEE151 pKa = 3.94EE152 pKa = 4.34LEE154 pKa = 4.0EE155 pKa = 5.29LITEE159 pKa = 4.72TVRR162 pKa = 11.84EE163 pKa = 4.43LSRR166 pKa = 11.84KK167 pKa = 9.59VYY169 pKa = 10.76VDD171 pKa = 3.72LEE173 pKa = 4.16EE174 pKa = 6.18AYY176 pKa = 10.74LEE178 pKa = 4.3QFTTEE183 pKa = 3.92NLIGLIEE190 pKa = 4.5ANDD193 pKa = 3.13WRR195 pKa = 11.84FNVDD199 pKa = 2.86EE200 pKa = 4.42YY201 pKa = 11.85GEE203 pKa = 4.2MSFSS207 pKa = 3.38

Molecular weight: 24.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1CKX6|A0A2P1CKX6_9CAUD Endolysin OS=Escherichia phage PDX OX=2099658 PE=3 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84KK3 pKa = 9.59LKK5 pKa = 10.88NFDD8 pKa = 3.47KK9 pKa = 11.23VDD11 pKa = 3.39VVLNNFYY18 pKa = 10.97YY19 pKa = 10.84CNNKK23 pKa = 8.89AVWWRR28 pKa = 11.84DD29 pKa = 2.89SSTDD33 pKa = 3.18KK34 pKa = 11.16VKK36 pKa = 10.96ARR38 pKa = 11.84FLQGHH43 pKa = 4.73WQGRR47 pKa = 11.84PVIKK51 pKa = 10.58IMGQLVAFKK60 pKa = 10.2NCRR63 pKa = 11.84RR64 pKa = 11.84VDD66 pKa = 3.75DD67 pKa = 3.96VEE69 pKa = 4.75EE70 pKa = 3.93ILSILRR76 pKa = 11.84HH77 pKa = 5.73NNLKK81 pKa = 10.86NSVDD85 pKa = 3.44KK86 pKa = 11.41

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

4432

86

1272

443.2

49.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.01 ± 0.603

0.88 ± 0.163

6.566 ± 0.331

5.912 ± 0.591

3.339 ± 0.244

8.258 ± 0.818

2.189 ± 0.138

5.551 ± 0.488

5.866 ± 0.923

6.656 ± 0.431

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.572 ± 0.315

5.641 ± 0.514

3.881 ± 0.312

3.791 ± 0.459

4.377 ± 0.418

5.641 ± 0.367

7.243 ± 0.923

7.717 ± 0.404

1.557 ± 0.186

4.355 ± 0.482
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski