Romboutsia hominis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Romboutsia

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2851 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P2BNR7|A0A2P2BNR7_9FIRM DNA polymerase I OS=Romboutsia hominis OX=1507512 GN=polA PE=3 SV=1
MM1 pKa = 7.28EE2 pKa = 4.59QLKK5 pKa = 11.14DD6 pKa = 3.36MLRR9 pKa = 11.84GFDD12 pKa = 4.23FVDD15 pKa = 4.2DD16 pKa = 3.85NPMIFFLIIGLVVLLLINSNGNLDD40 pKa = 4.77CFFDD44 pKa = 4.03QNNSLVWIILLVFLVFMLGRR64 pKa = 11.84NDD66 pKa = 3.45GCHH69 pKa = 7.56DD70 pKa = 4.44DD71 pKa = 4.23FCCC74 pKa = 5.33

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P2BVF4|A0A2P2BVF4_9FIRM Glutamate--tRNA ligase OS=Romboutsia hominis OX=1507512 GN=gltX PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 9.11TSNGRR28 pKa = 11.84NVLKK32 pKa = 10.51RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.37GRR39 pKa = 11.84KK40 pKa = 8.95RR41 pKa = 11.84LTHH44 pKa = 6.22

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2851

0

2851

844586

30

2131

296.2

33.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.309 ± 0.057

1.178 ± 0.02

5.769 ± 0.048

7.37 ± 0.058

4.162 ± 0.039

6.3 ± 0.051

1.362 ± 0.02

10.465 ± 0.074

9.337 ± 0.047

8.998 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.021

6.624 ± 0.047

2.679 ± 0.027

2.189 ± 0.021

3.124 ± 0.031

6.374 ± 0.046

4.771 ± 0.032

6.57 ± 0.043

0.547 ± 0.012

4.19 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski