Phormidium virus WMP3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Wumptrevirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5HL49|A5HL49_9CAUD PfWMP3_16 OS=Phormidium virus WMP3 OX=440250 GN=PfWMP3_16 PE=4 SV=1
MM1 pKa = 7.64ARR3 pKa = 11.84GEE5 pKa = 4.09VDD7 pKa = 4.58DD8 pKa = 5.06NLPYY12 pKa = 10.4WFVSSVRR19 pKa = 11.84DD20 pKa = 3.41RR21 pKa = 11.84ADD23 pKa = 3.01WDD25 pKa = 3.7SKK27 pKa = 10.78YY28 pKa = 10.72CLQFGLEE35 pKa = 4.03LWQLNEE41 pKa = 4.12EE42 pKa = 4.38LDD44 pKa = 4.23DD45 pKa = 3.94VKK47 pKa = 11.03QEE49 pKa = 3.98QEE51 pKa = 3.64

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5HL47|A5HL47_9CAUD DNA polymerase OS=Phormidium virus WMP3 OX=440250 GN=PfWMP3_14 PE=4 SV=1
MM1 pKa = 8.23DD2 pKa = 6.27DD3 pKa = 3.51KK4 pKa = 11.43LKK6 pKa = 10.96EE7 pKa = 4.1LFLTTDD13 pKa = 3.71LTYY16 pKa = 11.15YY17 pKa = 10.26QMAYY21 pKa = 10.85ALGTTYY27 pKa = 10.91KK28 pKa = 10.2VVHH31 pKa = 5.74GHH33 pKa = 4.91LTKK36 pKa = 10.82LFSKK40 pKa = 9.8EE41 pKa = 3.42EE42 pKa = 3.96RR43 pKa = 11.84KK44 pKa = 10.37ARR46 pKa = 11.84TSRR49 pKa = 11.84MLRR52 pKa = 11.84LARR55 pKa = 11.84TGEE58 pKa = 4.16KK59 pKa = 10.39NPMYY63 pKa = 9.72GTEE66 pKa = 4.1SNKK69 pKa = 10.25RR70 pKa = 11.84LDD72 pKa = 3.64VIADD76 pKa = 3.31GRR78 pKa = 11.84GYY80 pKa = 11.44LMVRR84 pKa = 11.84KK85 pKa = 7.13PTWYY89 pKa = 9.0TGRR92 pKa = 11.84KK93 pKa = 8.1GSRR96 pKa = 11.84HH97 pKa = 4.72VFQHH101 pKa = 5.51HH102 pKa = 5.15VVMCEE107 pKa = 3.63ALGLTQVPDD116 pKa = 3.99GFHH119 pKa = 5.34VHH121 pKa = 6.55HH122 pKa = 7.23VDD124 pKa = 4.56GDD126 pKa = 3.84KK127 pKa = 11.19TNNNINNLALLCDD140 pKa = 3.93SAHH143 pKa = 6.17SRR145 pKa = 11.84LHH147 pKa = 5.44SRR149 pKa = 11.84EE150 pKa = 3.66RR151 pKa = 11.84ATTISKK157 pKa = 10.12EE158 pKa = 4.09SRR160 pKa = 11.84VV161 pKa = 3.59

Molecular weight: 18.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

13245

36

1495

323.0

36.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.761 ± 0.343

0.725 ± 0.163

6.584 ± 0.197

5.821 ± 0.42

3.488 ± 0.227

6.553 ± 0.302

1.631 ± 0.237

3.398 ± 0.171

5.512 ± 0.355

9.022 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.812 ± 0.168

4.477 ± 0.229

4.273 ± 0.501

5.27 ± 0.492

5.881 ± 0.326

6.795 ± 0.339

7.565 ± 0.517

7.837 ± 0.277

1.133 ± 0.121

4.462 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski