Circoviridae 5 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples; Circoviridae LDMD-2013a

Average proteome isoelectric point is 8.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TMV0|S5TMV0_9CIRC ATP-dependent helicase Rep OS=Circoviridae 5 LDMD-2013 OX=1379709 PE=3 SV=1
MM1 pKa = 7.55YY2 pKa = 10.18YY3 pKa = 9.64PEE5 pKa = 5.08HH6 pKa = 6.38SHH8 pKa = 6.82VFLEE12 pKa = 3.94NWKK15 pKa = 8.43ITGKK19 pKa = 10.43RR20 pKa = 11.84SVTLHH25 pKa = 6.69PGEE28 pKa = 4.08QTTISHH34 pKa = 5.82SCSGYY39 pKa = 9.61FDD41 pKa = 3.57KK42 pKa = 11.29KK43 pKa = 10.53KK44 pKa = 10.74ISDD47 pKa = 4.01LAVGAEE53 pKa = 3.81PLVYY57 pKa = 10.71LKK59 pKa = 10.96GKK61 pKa = 8.77TSALIMRR68 pKa = 11.84VVGDD72 pKa = 3.75IGHH75 pKa = 7.22DD76 pKa = 3.44AEE78 pKa = 4.57TSLNCGYY85 pKa = 8.27TATKK89 pKa = 10.32LDD91 pKa = 3.9LVRR94 pKa = 11.84LKK96 pKa = 10.23TSTFRR101 pKa = 11.84FFNASKK107 pKa = 9.55QLKK110 pKa = 9.21YY111 pKa = 10.95DD112 pKa = 4.6FYY114 pKa = 11.43SQALGTASRR123 pKa = 11.84SVAKK127 pKa = 10.32LNHH130 pKa = 6.57DD131 pKa = 3.61MEE133 pKa = 5.24TDD135 pKa = 3.4VKK137 pKa = 11.34ADD139 pKa = 3.71DD140 pKa = 4.32DD141 pKa = 4.05

Molecular weight: 15.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5T675|S5T675_9CIRC Uncharacterized protein OS=Circoviridae 5 LDMD-2013 OX=1379709 PE=4 SV=1
MM1 pKa = 7.39AGIVFFEE8 pKa = 4.21AAEE11 pKa = 4.27LGLPVLEE18 pKa = 4.81AAMEE22 pKa = 4.25ASEE25 pKa = 4.42FVHH28 pKa = 6.62PFARR32 pKa = 11.84QTVRR36 pKa = 11.84TQVGAGAFAAGVNKK50 pKa = 8.87LTSGPDD56 pKa = 3.32HH57 pKa = 6.69KK58 pKa = 10.41RR59 pKa = 11.84KK60 pKa = 10.73SMVKK64 pKa = 8.81TRR66 pKa = 11.84SHH68 pKa = 6.2TKK70 pKa = 10.44NEE72 pKa = 3.69LAALRR77 pKa = 11.84TIAAQNDD84 pKa = 3.71LMKK87 pKa = 9.85TQTQMRR93 pKa = 11.84TFVPTSFKK101 pKa = 10.09KK102 pKa = 10.25RR103 pKa = 11.84KK104 pKa = 9.92RR105 pKa = 11.84FDD107 pKa = 3.37KK108 pKa = 11.08LSDD111 pKa = 3.36PGIWQTRR118 pKa = 11.84RR119 pKa = 11.84QVEE122 pKa = 4.18NVILTGNTNAATYY135 pKa = 7.34TVSYY139 pKa = 9.92IGRR142 pKa = 11.84NSDD145 pKa = 4.58LLNLMDD151 pKa = 6.74DD152 pKa = 3.86EE153 pKa = 4.7FKK155 pKa = 11.08KK156 pKa = 10.68IGGSNDD162 pKa = 3.25IEE164 pKa = 4.38HH165 pKa = 6.86VDD167 pKa = 3.54YY168 pKa = 9.42TAASMAAFSPILQFKK183 pKa = 9.24IRR185 pKa = 11.84QVLHH189 pKa = 5.78MKK191 pKa = 10.37NNYY194 pKa = 8.45XIYY197 pKa = 8.24CQXYY201 pKa = 9.59IFLIVLXKK209 pKa = 10.21IIVLLKK215 pKa = 9.33TIPP218 pKa = 3.26

Molecular weight: 24.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

641

141

282

213.7

24.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.02 ± 1.13

1.404 ± 0.403

6.24 ± 0.857

4.524 ± 0.224

4.68 ± 0.326

6.396 ± 0.581

2.496 ± 0.593

4.212 ± 1.075

8.424 ± 0.44

8.424 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.354

3.744 ± 0.524

3.432 ± 0.505

3.432 ± 0.529

5.616 ± 0.751

6.084 ± 1.02

7.8 ± 0.251

6.552 ± 0.13

1.872 ± 0.854

4.368 ± 0.641
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski