Influenza A virus (A/northern shoveler/California/JN1447/2007(H7N2))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H7N2 subtype

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4UF17|E4UF17_9INFA Matrix protein 1 OS=Influenza A virus (A/northern shoveler/California/JN1447/2007(H7N2)) OX=686561 GN=M1 PE=3 SV=1
MM1 pKa = 7.58SLLTEE6 pKa = 4.21VATPTRR12 pKa = 11.84SGWEE16 pKa = 4.24CRR18 pKa = 11.84CSDD21 pKa = 3.53SSDD24 pKa = 4.08PIVIVASIIGILHH37 pKa = 7.1LILWILDD44 pKa = 3.39RR45 pKa = 11.84LFFKK49 pKa = 10.78CIYY52 pKa = 9.95RR53 pKa = 11.84RR54 pKa = 11.84LKK56 pKa = 10.55YY57 pKa = 10.03GLKK60 pKa = 10.09RR61 pKa = 11.84GPSTEE66 pKa = 4.68GIPEE70 pKa = 4.21SMRR73 pKa = 11.84EE74 pKa = 4.03EE75 pKa = 4.08YY76 pKa = 10.41RR77 pKa = 11.84QEE79 pKa = 3.7QQNAVGVDD87 pKa = 3.33DD88 pKa = 3.95GHH90 pKa = 6.3FVNIEE95 pKa = 3.83LEE97 pKa = 4.08

Molecular weight: 11.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4UF18|E4UF18_9INFA Neuraminidase OS=Influenza A virus (A/northern shoveler/California/JN1447/2007(H7N2)) OX=686561 GN=NA PE=3 SV=1
MM1 pKa = 7.58SLLTEE6 pKa = 3.91VATYY10 pKa = 10.42VLSIVPSGPLKK21 pKa = 11.07AEE23 pKa = 3.62IAQRR27 pKa = 11.84LEE29 pKa = 3.96DD30 pKa = 3.83VFAGKK35 pKa = 8.33NTDD38 pKa = 4.04LEE40 pKa = 5.12ALMEE44 pKa = 4.21WLKK47 pKa = 9.79TRR49 pKa = 11.84PILSPLTKK57 pKa = 10.4GILGFVFTLTVPSEE71 pKa = 4.2RR72 pKa = 11.84GLQRR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84FVQNALNGNGDD89 pKa = 4.31PNNMDD94 pKa = 3.72RR95 pKa = 11.84AVKK98 pKa = 10.36LYY100 pKa = 10.56RR101 pKa = 11.84KK102 pKa = 9.6LKK104 pKa = 10.61RR105 pKa = 11.84EE106 pKa = 3.99ITFHH110 pKa = 5.75GAKK113 pKa = 9.45EE114 pKa = 4.07VALSYY119 pKa = 10.16STGALASCMGLIYY132 pKa = 10.92NRR134 pKa = 11.84MGTVTTEE141 pKa = 3.79VAFGLVCATCEE152 pKa = 3.92QIADD156 pKa = 3.85SQHH159 pKa = 6.13RR160 pKa = 11.84SHH162 pKa = 6.73RR163 pKa = 11.84QMVTTTNPLIRR174 pKa = 11.84HH175 pKa = 5.69EE176 pKa = 4.07NRR178 pKa = 11.84MVLASTTAKK187 pKa = 10.69AMEE190 pKa = 4.27QIAGSSEE197 pKa = 3.83QAAEE201 pKa = 4.01AMEE204 pKa = 4.52VASQARR210 pKa = 11.84QMVQAMRR217 pKa = 11.84TIGTHH222 pKa = 6.14PSSSAGLKK230 pKa = 10.33DD231 pKa = 4.47DD232 pKa = 5.24LLEE235 pKa = 4.59NLQAYY240 pKa = 6.88QKK242 pKa = 10.84RR243 pKa = 11.84MGMQMQRR250 pKa = 11.84FKK252 pKa = 11.47

Molecular weight: 27.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

2986

97

759

373.3

41.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.062 ± 0.658

1.942 ± 0.532

4.756 ± 0.334

6.631 ± 0.548

3.617 ± 0.283

7.569 ± 0.541

1.541 ± 0.118

6.865 ± 0.469

4.488 ± 0.416

7.77 ± 0.803

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.751 ± 0.533

5.023 ± 0.509

3.617 ± 0.272

4.655 ± 0.388

7.535 ± 0.795

7.837 ± 0.548

6.631 ± 0.316

5.928 ± 0.714

1.541 ± 0.241

2.244 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski