Pseudomonas phage JG054

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Queuovirinae; Nipunavirus; unclassified Nipunavirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4GY99|A0A2H4GY99_9CAUD Uncharacterized protein OS=Pseudomonas phage JG054 OX=1970800 GN=JG054_00055 PE=4 SV=1
MM1 pKa = 7.79CEE3 pKa = 5.04LDD5 pKa = 5.51DD6 pKa = 4.6PMAPARR12 pKa = 11.84GIVNGVLFSIPLWAVIIAVAYY33 pKa = 7.27WALL36 pKa = 3.44

Molecular weight: 3.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4GYC5|A0A2H4GYC5_9CAUD Putative exonuclease RecB OS=Pseudomonas phage JG054 OX=1970800 GN=JG054_00042 PE=4 SV=1
MM1 pKa = 7.65IPRR4 pKa = 11.84FPMSPEE10 pKa = 3.45TAKK13 pKa = 11.02AIMEE17 pKa = 3.96EE18 pKa = 4.05HH19 pKa = 5.72EE20 pKa = 4.31RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 9.83ARR26 pKa = 11.84EE27 pKa = 3.31EE28 pKa = 3.79RR29 pKa = 11.84KK30 pKa = 9.46RR31 pKa = 11.84AKK33 pKa = 10.4LLAKK37 pKa = 9.83EE38 pKa = 4.36LEE40 pKa = 4.04AALRR44 pKa = 11.84GEE46 pKa = 4.29QGPAQPRR53 pKa = 11.84AYY55 pKa = 8.66WRR57 pKa = 11.84WPMGIGAHH65 pKa = 6.95AIALAAIAAAALLGWLLLRR84 pKa = 5.08

Molecular weight: 9.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

18091

36

976

244.5

27.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.652 ± 0.412

1.205 ± 0.155

5.804 ± 0.181

6.042 ± 0.283

3.56 ± 0.148

7.705 ± 0.287

2.001 ± 0.199

5.08 ± 0.184

4.82 ± 0.22

8.181 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.747 ± 0.138

3.908 ± 0.163

4.676 ± 0.264

4.643 ± 0.36

5.986 ± 0.241

5.622 ± 0.237

5.826 ± 0.292

6.793 ± 0.244

1.763 ± 0.131

2.985 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski