Staphylococcus phage SA537ruMSSAST97

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Peeveelvirus; unclassified Peeveelvirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345AQS5|A0A345AQS5_9CAUD Uncharacterized protein OS=Staphylococcus phage SA537ruMSSAST97 OX=2268623 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.25NYY4 pKa = 10.4YY5 pKa = 9.86HH6 pKa = 7.24LLSFDD11 pKa = 5.04DD12 pKa = 5.61DD13 pKa = 4.5LANDD17 pKa = 4.17AANDD21 pKa = 4.01LLKK24 pKa = 10.8EE25 pKa = 4.08GWDD28 pKa = 3.34IVHH31 pKa = 6.66VGTKK35 pKa = 9.51LVKK38 pKa = 10.36ILDD41 pKa = 3.92NGQAYY46 pKa = 10.82YY47 pKa = 8.91NTEE50 pKa = 3.97YY51 pKa = 11.47VLGGTKK57 pKa = 9.45NQYY60 pKa = 9.36EE61 pKa = 4.83KK62 pKa = 11.2YY63 pKa = 10.75LEE65 pKa = 4.8DD66 pKa = 4.04CQQSEE71 pKa = 4.31LDD73 pKa = 3.76YY74 pKa = 11.3FF75 pKa = 4.23

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345AQW8|A0A345AQW8_9CAUD Uncharacterized protein OS=Staphylococcus phage SA537ruMSSAST97 OX=2268623 PE=4 SV=1
MM1 pKa = 8.25PITLIVGALVGIGIAFYY18 pKa = 10.02QAYY21 pKa = 9.25KK22 pKa = 10.26RR23 pKa = 11.84SEE25 pKa = 3.95TFRR28 pKa = 11.84NIVNQAISGVANAFKK43 pKa = 10.38AAKK46 pKa = 9.72LALQGFFDD54 pKa = 5.0LFKK57 pKa = 10.77GDD59 pKa = 3.29SKK61 pKa = 11.62GAVTLEE67 pKa = 4.39KK68 pKa = 10.08IFPPEE73 pKa = 3.69TVAGIQNVVNTIRR86 pKa = 11.84TTFFKK91 pKa = 11.04VVDD94 pKa = 5.01AIVGFAKK101 pKa = 10.66EE102 pKa = 4.13IGAQLASFWKK112 pKa = 10.26EE113 pKa = 3.52NGSEE117 pKa = 3.87ITQALQNIAGFIKK130 pKa = 10.05ATFEE134 pKa = 4.49FIFNFIIKK142 pKa = 9.89PIMFAIWQVMQFIWPAVKK160 pKa = 10.39ALIVSTWEE168 pKa = 3.89NIKK171 pKa = 10.86GVIQGAINIILGIIKK186 pKa = 9.74VFSSLFTGNWRR197 pKa = 11.84GVWDD201 pKa = 5.1GIVMILKK208 pKa = 8.67GTVQLIWNLIQLWFIGKK225 pKa = 8.65ILGVVRR231 pKa = 11.84YY232 pKa = 9.14FGGLLKK238 pKa = 10.93GLITIIWVAIIGVFKK253 pKa = 10.88KK254 pKa = 10.63SLSAIWNATKK264 pKa = 10.51SIFGFLFNSVKK275 pKa = 10.81SIFTNMKK282 pKa = 9.13NWLSSTWNNIKK293 pKa = 10.89SNTVGKK299 pKa = 10.05AHH301 pKa = 6.79SLFTGVRR308 pKa = 11.84SKK310 pKa = 9.99FTSLWNATKK319 pKa = 10.52DD320 pKa = 2.9IFTKK324 pKa = 10.34LRR326 pKa = 11.84NWMSNIWNSIKK337 pKa = 10.99DD338 pKa = 3.66NTVGIAGRR346 pKa = 11.84LWDD349 pKa = 3.65RR350 pKa = 11.84VRR352 pKa = 11.84NIFGSMRR359 pKa = 11.84DD360 pKa = 3.59GLKK363 pKa = 10.75SIIGKK368 pKa = 9.69IKK370 pKa = 10.09DD371 pKa = 3.91HH372 pKa = 6.74IGGMVDD378 pKa = 3.0AVKK381 pKa = 10.61RR382 pKa = 11.84GLNKK386 pKa = 10.47LIEE389 pKa = 4.41GLNWVGGKK397 pKa = 10.27LGMDD401 pKa = 5.36KK402 pKa = 10.4IPKK405 pKa = 8.99LHH407 pKa = 6.73TGTEE411 pKa = 4.34HH412 pKa = 5.61THH414 pKa = 4.28TTTRR418 pKa = 11.84LVKK421 pKa = 10.0NGKK424 pKa = 8.59IARR427 pKa = 11.84DD428 pKa = 3.55TFATVGDD435 pKa = 3.94KK436 pKa = 11.29GRR438 pKa = 11.84GNGPNGFRR446 pKa = 11.84NEE448 pKa = 4.12MIEE451 pKa = 4.07FPNGKK456 pKa = 9.2RR457 pKa = 11.84VLTPNTDD464 pKa = 2.88TTAYY468 pKa = 9.76LPKK471 pKa = 10.41GSKK474 pKa = 9.93VYY476 pKa = 10.88NGAQTYY482 pKa = 11.36SMLNGTLPRR491 pKa = 11.84FSIGTMWKK499 pKa = 9.9DD500 pKa = 3.23IKK502 pKa = 10.95SGASSAFNWTKK513 pKa = 11.04DD514 pKa = 3.55QIGKK518 pKa = 6.62GTKK521 pKa = 8.59WLGDD525 pKa = 3.52KK526 pKa = 11.04VGDD529 pKa = 3.83VMDD532 pKa = 6.29FIDD535 pKa = 5.68NPGKK539 pKa = 10.35LLNYY543 pKa = 9.05VLQAFGVDD551 pKa = 4.58FSSLTKK557 pKa = 10.72GMGIAGDD564 pKa = 3.52ITKK567 pKa = 10.45AAWSKK572 pKa = 10.35IKK574 pKa = 10.52KK575 pKa = 9.78SAIKK579 pKa = 9.68WLEE582 pKa = 3.74DD583 pKa = 3.04AFAEE587 pKa = 4.44SGDD590 pKa = 3.93GGVLDD595 pKa = 4.17MNKK598 pKa = 9.58LRR600 pKa = 11.84YY601 pKa = 9.58LYY603 pKa = 10.75GHH605 pKa = 6.3TAAYY609 pKa = 8.63TRR611 pKa = 11.84EE612 pKa = 4.0TGRR615 pKa = 11.84PFHH618 pKa = 6.98EE619 pKa = 5.15GLDD622 pKa = 3.37FDD624 pKa = 5.43YY625 pKa = 10.49IYY627 pKa = 10.86EE628 pKa = 4.43PVPSTINGRR637 pKa = 11.84AQVMPFHH644 pKa = 6.69NGGYY648 pKa = 9.1GKK650 pKa = 9.26WVKK653 pKa = 9.66IVKK656 pKa = 9.85GALEE660 pKa = 4.22VIYY663 pKa = 10.74AHH665 pKa = 6.91LSKK668 pKa = 11.25YY669 pKa = 9.43KK670 pKa = 10.69VKK672 pKa = 9.65TGQQVRR678 pKa = 11.84VGQTVGISGNTGFSTGPHH696 pKa = 5.68LHH698 pKa = 7.05YY699 pKa = 10.8EE700 pKa = 4.1MRR702 pKa = 11.84WNGRR706 pKa = 11.84HH707 pKa = 6.33RR708 pKa = 11.84DD709 pKa = 3.7PLPWLRR715 pKa = 11.84KK716 pKa = 9.28NNGGGKK722 pKa = 8.03STPGGNGAANARR734 pKa = 11.84RR735 pKa = 11.84AIKK738 pKa = 10.02AAQNILGGRR747 pKa = 11.84YY748 pKa = 8.1KK749 pKa = 10.85ASWITNEE756 pKa = 3.8MMRR759 pKa = 11.84VASRR763 pKa = 11.84EE764 pKa = 4.02SNYY767 pKa = 9.28TANAVNNWDD776 pKa = 3.53SNARR780 pKa = 11.84AGIPSRR786 pKa = 11.84GMFQMIDD793 pKa = 3.41PSFRR797 pKa = 11.84AYY799 pKa = 10.42AKK801 pKa = 10.18SGYY804 pKa = 9.89NNPLNPTHH812 pKa = 6.01QAISAMRR819 pKa = 11.84YY820 pKa = 9.01IVGKK824 pKa = 8.54WVPRR828 pKa = 11.84TGSWRR833 pKa = 11.84AAFKK837 pKa = 10.7RR838 pKa = 11.84AGDD841 pKa = 3.57YY842 pKa = 10.87AYY844 pKa = 9.48ATGGKK849 pKa = 9.28VYY851 pKa = 10.84NGLYY855 pKa = 10.37HH856 pKa = 7.48LGEE859 pKa = 4.18EE860 pKa = 5.76GYY862 pKa = 9.66PEE864 pKa = 4.64WIIPTDD870 pKa = 3.52PSRR873 pKa = 11.84ANEE876 pKa = 3.92AHH878 pKa = 7.18KK879 pKa = 10.95LLALAANDD887 pKa = 3.04IDD889 pKa = 4.02NRR891 pKa = 11.84SKK893 pKa = 11.26NKK895 pKa = 10.15RR896 pKa = 11.84PNNLPNPSISNSDD909 pKa = 2.99TNYY912 pKa = 10.16IHH914 pKa = 6.64TLEE917 pKa = 4.51NKK919 pKa = 9.81LDD921 pKa = 3.73AVINCLVSLVEE932 pKa = 4.34SNQVIADD939 pKa = 3.63KK940 pKa = 10.76DD941 pKa = 3.8YY942 pKa = 10.92EE943 pKa = 4.23PVINKK948 pKa = 9.14YY949 pKa = 9.6VFEE952 pKa = 5.09DD953 pKa = 3.95EE954 pKa = 4.6VNNSIDD960 pKa = 3.0KK961 pKa = 10.58RR962 pKa = 11.84EE963 pKa = 3.96RR964 pKa = 11.84HH965 pKa = 5.3EE966 pKa = 4.02STRR969 pKa = 11.84VRR971 pKa = 11.84FRR973 pKa = 11.84RR974 pKa = 11.84GGTIII979 pKa = 4.08

Molecular weight: 108.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12850

40

1260

191.8

22.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.432 ± 0.334

0.56 ± 0.094

6.272 ± 0.32

7.922 ± 0.53

4.086 ± 0.236

5.362 ± 0.448

1.681 ± 0.151

8.016 ± 0.322

9.3 ± 0.327

8.14 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.238

6.911 ± 0.298

2.42 ± 0.176

3.977 ± 0.246

4.389 ± 0.198

5.977 ± 0.258

5.821 ± 0.215

5.844 ± 0.211

1.074 ± 0.19

4.304 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski