bacterium 0.1xD8-82

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4335 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9ATI9|A0A3A9ATI9_9BACT Uncharacterized protein OS=bacterium 0.1xD8-82 OX=2320100 GN=D7V94_12135 PE=4 SV=1
MM1 pKa = 7.21KK2 pKa = 10.16RR3 pKa = 11.84KK4 pKa = 9.29IVAVLLSAAMILSMTACGNSSSGSNASEE32 pKa = 4.26GAAQDD37 pKa = 3.52TAQAADD43 pKa = 3.82TSNEE47 pKa = 3.76DD48 pKa = 3.39AAADD52 pKa = 3.81AQDD55 pKa = 4.54ADD57 pKa = 4.57AEE59 pKa = 4.26DD60 pKa = 4.19SADD63 pKa = 3.61EE64 pKa = 4.55GASSSATADD73 pKa = 3.57FSGVKK78 pKa = 10.34AEE80 pKa = 4.1IDD82 pKa = 3.01IDD84 pKa = 3.78GTIIGDD90 pKa = 3.64ALTTFEE96 pKa = 4.65GKK98 pKa = 10.0VEE100 pKa = 4.06EE101 pKa = 4.47FNNLTGAQLEE111 pKa = 4.36LVEE114 pKa = 5.47NGDD117 pKa = 3.61DD118 pKa = 3.94HH119 pKa = 6.41EE120 pKa = 7.63AIMKK124 pKa = 8.9TRR126 pKa = 11.84MASNDD131 pKa = 3.73MPDD134 pKa = 2.73MFTTHH139 pKa = 5.21GWSTIRR145 pKa = 11.84YY146 pKa = 9.22NEE148 pKa = 3.71FCYY151 pKa = 10.72DD152 pKa = 3.8LSGEE156 pKa = 4.25SWVSDD161 pKa = 3.54MADD164 pKa = 2.84AAAAVVTDD172 pKa = 4.6PNGKK176 pKa = 8.85ICTCPLTEE184 pKa = 3.97WVYY187 pKa = 11.2GIAFNNTIMEE197 pKa = 4.79DD198 pKa = 3.41NGIDD202 pKa = 3.28MYY204 pKa = 11.3AIKK207 pKa = 9.08TWDD210 pKa = 4.14DD211 pKa = 3.28LTAALQTLKK220 pKa = 10.86DD221 pKa = 3.65AGITPISVGGKK232 pKa = 7.85STGSLGGYY240 pKa = 10.18LEE242 pKa = 4.31MSNVFYY248 pKa = 11.06SADD251 pKa = 2.97GAMYY255 pKa = 10.58DD256 pKa = 5.19GGAQLQDD263 pKa = 3.06GSFSFVEE270 pKa = 4.14HH271 pKa = 6.89PEE273 pKa = 4.21IIARR277 pKa = 11.84FADD280 pKa = 4.44LYY282 pKa = 11.33DD283 pKa = 3.41KK284 pKa = 11.54GFFIEE289 pKa = 5.57DD290 pKa = 4.61LFTADD295 pKa = 4.11PDD297 pKa = 3.84TARR300 pKa = 11.84NYY302 pKa = 10.15VATGQAAMLLWGSPEE317 pKa = 3.92YY318 pKa = 10.9VDD320 pKa = 5.59LMKK323 pKa = 10.14TANPDD328 pKa = 3.31NEE330 pKa = 4.27FGIIPVPAVEE340 pKa = 4.61EE341 pKa = 4.5GGKK344 pKa = 8.84PAYY347 pKa = 9.16TVGEE351 pKa = 4.43GTAIAISSSTEE362 pKa = 3.7NLEE365 pKa = 3.99LCQAFLNFLTDD376 pKa = 3.6PEE378 pKa = 4.4TLKK381 pKa = 11.09VYY383 pKa = 10.63VDD385 pKa = 3.36STGAVSGFKK394 pKa = 10.03TISQDD399 pKa = 3.5DD400 pKa = 4.41TYY402 pKa = 11.93SLNKK406 pKa = 10.0YY407 pKa = 9.56NEE409 pKa = 4.6SIEE412 pKa = 4.06KK413 pKa = 9.44VANITYY419 pKa = 9.87TNFFDD424 pKa = 5.57RR425 pKa = 11.84EE426 pKa = 4.14YY427 pKa = 10.63LPSGMWNYY435 pKa = 9.37MSEE438 pKa = 4.78SIAQLFNCNVGEE450 pKa = 4.2ASGKK454 pKa = 8.5VDD456 pKa = 3.65EE457 pKa = 4.66VAEE460 pKa = 4.14YY461 pKa = 8.65MQQAYY466 pKa = 8.27EE467 pKa = 4.03QLYY470 pKa = 8.42ATNNEE475 pKa = 3.98

Molecular weight: 51.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9AWV5|A0A3A9AWV5_9BACT RNase H domain-containing protein OS=bacterium 0.1xD8-82 OX=2320100 GN=D7V94_08265 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 9.32KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4335

0

4335

1345899

25

1999

310.5

34.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.337 ± 0.037

1.597 ± 0.016

5.447 ± 0.033

7.721 ± 0.046

4.363 ± 0.031

7.171 ± 0.031

1.7 ± 0.016

7.364 ± 0.035

6.807 ± 0.034

9.114 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.176 ± 0.021

4.346 ± 0.028

3.252 ± 0.023

3.221 ± 0.02

4.659 ± 0.026

5.785 ± 0.032

4.986 ± 0.03

6.572 ± 0.031

1.031 ± 0.015

4.351 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski