Lactobacillus oligofermentans DSM 15707 = LMG 22743

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Paucilactobacillus; Paucilactobacillus oligofermentans

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R1RFH8|A0A0R1RFH8_9LACO Transcriptional regulator OS=Lactobacillus oligofermentans DSM 15707 = LMG 22743 OX=1423778 GN=FC70_GL001241 PE=3 SV=1
MM1 pKa = 7.76PSIFLTTNYY10 pKa = 9.99QKK12 pKa = 11.35LNDD15 pKa = 4.66DD16 pKa = 3.87EE17 pKa = 5.12TDD19 pKa = 3.67DD20 pKa = 4.52FLDD23 pKa = 4.03EE24 pKa = 5.38AMSDD28 pKa = 4.13LNRR31 pKa = 11.84QGLNTSEE38 pKa = 3.66WTYY41 pKa = 11.28DD42 pKa = 2.96QSTIVFNALMKK53 pKa = 10.45YY54 pKa = 10.63DD55 pKa = 4.61EE56 pKa = 5.32IDD58 pKa = 3.82PEE60 pKa = 4.35LLQDD64 pKa = 4.0PNEE67 pKa = 4.1FDD69 pKa = 4.21ALEE72 pKa = 4.68DD73 pKa = 4.81DD74 pKa = 4.53IEE76 pKa = 4.19IQRR79 pKa = 11.84RR80 pKa = 11.84EE81 pKa = 4.21IDD83 pKa = 3.29HH84 pKa = 7.62DD85 pKa = 4.67DD86 pKa = 3.79YY87 pKa = 11.79PGDD90 pKa = 4.19EE91 pKa = 4.62DD92 pKa = 6.35DD93 pKa = 6.13KK94 pKa = 11.92DD95 pKa = 3.56FDD97 pKa = 4.73LAGKK101 pKa = 10.29SIDD104 pKa = 3.38GTNYY108 pKa = 10.77DD109 pKa = 3.63NWNHH113 pKa = 6.74PDD115 pKa = 3.62AEE117 pKa = 5.22DD118 pKa = 3.82YY119 pKa = 11.32DD120 pKa = 4.27ADD122 pKa = 4.46EE123 pKa = 4.11YY124 pKa = 10.21TKK126 pKa = 10.92YY127 pKa = 10.9YY128 pKa = 10.47SNHH131 pKa = 5.26

Molecular weight: 15.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R1RLZ0|A0A0R1RLZ0_9LACO Thymidylate kinase OS=Lactobacillus oligofermentans DSM 15707 = LMG 22743 OX=1423778 GN=tmk PE=3 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84FIQGIFGGYY11 pKa = 8.71VSNANAIIATTAPRR25 pKa = 11.84EE26 pKa = 3.97KK27 pKa = 10.21SGRR30 pKa = 11.84ALGTLMAGLTGGSLLGPLLGGALASMFSYY59 pKa = 10.87RR60 pKa = 11.84MTFLITGLLLFIVFIFSWLFVTEE83 pKa = 4.63NNFKK87 pKa = 9.33PTTAKK92 pKa = 10.58KK93 pKa = 9.03LQKK96 pKa = 10.16ASGVISVLKK105 pKa = 9.42NPQMIFGMFITTLIIQASNNSITPIISLYY134 pKa = 10.22VRR136 pKa = 11.84EE137 pKa = 4.41LMHH140 pKa = 5.9NTGNVTLVSGVIAAVPGLATMIVAPRR166 pKa = 11.84FGEE169 pKa = 4.03LGDD172 pKa = 4.17RR173 pKa = 11.84IGTEE177 pKa = 3.79RR178 pKa = 11.84VLTIGFVLAICFFIPTAFVTNIWQLGILRR207 pKa = 11.84FLIGISNATMIPQVQTLLTKK227 pKa = 8.63NTPPEE232 pKa = 3.7VTGRR236 pKa = 11.84IFSWNQSFQSFGNVIGPLLGAFISGIFNYY265 pKa = 10.23SAVFISTAILVAINFVLFRR284 pKa = 11.84INVVNANKK292 pKa = 10.43

Molecular weight: 31.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1735

0

1735

536333

50

2788

309.1

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.378 ± 0.067

0.433 ± 0.016

5.961 ± 0.058

5.883 ± 0.075

4.373 ± 0.053

6.595 ± 0.059

1.919 ± 0.028

7.913 ± 0.067

6.527 ± 0.058

9.363 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.829 ± 0.03

5.331 ± 0.044

3.255 ± 0.034

4.268 ± 0.043

3.493 ± 0.051

6.722 ± 0.168

6.228 ± 0.065

7.1 ± 0.047

0.979 ± 0.022

3.44 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski