Hymenobacter actinosclerus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3677 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0GZR0|A0A1I0GZR0_9BACT Uncharacterized protein OS=Hymenobacter actinosclerus OX=82805 GN=SAMN04487998_2675 PE=4 SV=1
MM1 pKa = 7.72LSTHH5 pKa = 6.57HH6 pKa = 6.96PAGLPPAADD15 pKa = 3.85LQRR18 pKa = 11.84LGQALAMLDD27 pKa = 4.6AINSPDD33 pKa = 3.49PEE35 pKa = 4.19YY36 pKa = 10.62RR37 pKa = 11.84YY38 pKa = 8.84YY39 pKa = 10.62TYY41 pKa = 10.82DD42 pKa = 3.46PHH44 pKa = 7.35WDD46 pKa = 3.32ADD48 pKa = 3.97EE49 pKa = 4.5QLLEE53 pKa = 4.22MNDD56 pKa = 3.96GEE58 pKa = 4.62GDD60 pKa = 3.44QLLVLFRR67 pKa = 11.84PEE69 pKa = 4.16GCCINGSLSGHH80 pKa = 5.89EE81 pKa = 4.22PADD84 pKa = 3.55KK85 pKa = 10.74KK86 pKa = 10.97QLTRR90 pKa = 11.84GLPAAFEE97 pKa = 4.06EE98 pKa = 5.0FMFGEE103 pKa = 4.57PVEE106 pKa = 4.76SIGTSFCLWYY116 pKa = 9.45TPAHH120 pKa = 6.12GWQTGVVDD128 pKa = 3.85EE129 pKa = 5.43AEE131 pKa = 4.71DD132 pKa = 4.47DD133 pKa = 4.1GSEE136 pKa = 3.92DD137 pKa = 3.78LMAIFDD143 pKa = 4.34QNPEE147 pKa = 4.21TYY149 pKa = 10.94ADD151 pKa = 3.69WANGYY156 pKa = 9.57YY157 pKa = 10.18LDD159 pKa = 3.8EE160 pKa = 4.55TEE162 pKa = 5.17RR163 pKa = 11.84EE164 pKa = 4.12PLEE167 pKa = 4.29TADD170 pKa = 3.21VARR173 pKa = 11.84FYY175 pKa = 10.77QHH177 pKa = 6.84EE178 pKa = 4.75PLTQATAQALNPDD191 pKa = 3.73LTDD194 pKa = 3.59WPQLAADD201 pKa = 4.83AEE203 pKa = 4.74QIGYY207 pKa = 8.49PHH209 pKa = 6.83EE210 pKa = 4.59LKK212 pKa = 10.9SS213 pKa = 3.48

Molecular weight: 23.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0J2E3|A0A1I0J2E3_9BACT NTE family protein OS=Hymenobacter actinosclerus OX=82805 GN=SAMN04487998_3617 PE=4 SV=1
MM1 pKa = 6.81RR2 pKa = 11.84TSRR5 pKa = 11.84RR6 pKa = 11.84FRR8 pKa = 11.84WLKK11 pKa = 10.79AKK13 pKa = 10.44LAGALVLLTLVAWGSWQEE31 pKa = 3.92PSLHH35 pKa = 6.41EE36 pKa = 4.08FTPVTEE42 pKa = 4.82FVTLTAPDD50 pKa = 4.23LQPGAAAEE58 pKa = 3.95QLQARR63 pKa = 11.84ALALPGVTACAVRR76 pKa = 11.84PEE78 pKa = 4.15KK79 pKa = 10.5QLLTLAYY86 pKa = 9.94RR87 pKa = 11.84PAEE90 pKa = 3.95LSAGQLQQRR99 pKa = 11.84LNLSALPQPEE109 pKa = 4.4LSAEE113 pKa = 3.9AAARR117 pKa = 11.84QCPVPPGYY125 pKa = 10.5VQALEE130 pKa = 4.0RR131 pKa = 11.84LRR133 pKa = 11.84FAFNLRR139 pKa = 11.84RR140 pKa = 11.84LFVRR144 pKa = 11.84LL145 pKa = 3.43

Molecular weight: 16.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3677

0

3677

1251588

37

4250

340.4

37.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.915 ± 0.059

0.678 ± 0.011

4.962 ± 0.03

5.416 ± 0.04

4.007 ± 0.022

7.649 ± 0.04

1.994 ± 0.021

4.194 ± 0.039

3.725 ± 0.042

11.545 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.841 ± 0.019

3.536 ± 0.034

5.458 ± 0.034

4.728 ± 0.032

6.531 ± 0.038

5.296 ± 0.03

5.939 ± 0.043

6.988 ± 0.032

1.186 ± 0.016

3.412 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski