Candidatus Tokpelaia sp. JSC188

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiales incertae sedis; Candidatus Tokpelaia; unclassified Candidatus Tokpelaia

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1066 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A368A1W4|A0A368A1W4_9RHIZ Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Candidatus Tokpelaia sp. JSC188 OX=2079009 GN=aceF PE=3 SV=1
MM1 pKa = 7.45NEE3 pKa = 3.85ADD5 pKa = 5.34AIDD8 pKa = 4.48LVASAIWTVLLVSGPAVFSAMLVGIGIALFQALTQIQEE46 pKa = 3.97MTLTFIPKK54 pKa = 9.31IVVILVVLVFSAPFIGAQIYY74 pKa = 7.63TFTQYY79 pKa = 10.83VYY81 pKa = 11.25GRR83 pKa = 11.84IEE85 pKa = 3.92SGFF88 pKa = 3.5

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368A4B9|A0A368A4B9_9RHIZ Beta-lactamase domain-containing protein OS=Candidatus Tokpelaia sp. JSC188 OX=2079009 GN=JSC188_000102 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84KK3 pKa = 9.13AASCGFKK10 pKa = 10.34QMKK13 pKa = 8.71RR14 pKa = 11.84TYY16 pKa = 9.79QPSKK20 pKa = 7.51TVRR23 pKa = 11.84KK24 pKa = 9.12RR25 pKa = 11.84RR26 pKa = 11.84HH27 pKa = 4.35GFRR30 pKa = 11.84ARR32 pKa = 11.84MATSSGRR39 pKa = 11.84RR40 pKa = 11.84IITARR45 pKa = 11.84RR46 pKa = 11.84ARR48 pKa = 11.84GRR50 pKa = 11.84KK51 pKa = 9.03RR52 pKa = 11.84LSAA55 pKa = 4.03

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1066

0

1066

338176

30

2857

317.2

35.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.491 ± 0.063

1.148 ± 0.024

5.152 ± 0.055

5.854 ± 0.079

4.385 ± 0.06

6.864 ± 0.067

2.267 ± 0.032

8.621 ± 0.058

5.659 ± 0.063

9.767 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.033

4.247 ± 0.038

3.808 ± 0.037

3.374 ± 0.036

6.019 ± 0.063

6.372 ± 0.052

5.12 ± 0.042

6.373 ± 0.059

1.055 ± 0.028

2.776 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski