Acinetobacter phage vB_ApiM_fHyAci03

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Twarogvirinae; Lazarusvirus; Acinetobacter virus fHyAci03

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 247 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345AUN1|A0A345AUN1_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_ApiM_fHyAci03 OX=2269366 GN=Ac3_045 PE=4 SV=1
MM1 pKa = 7.59LNIQLPSLQQIINQFLADD19 pKa = 4.31FNILDD24 pKa = 3.87GNFVHH29 pKa = 7.1KK30 pKa = 9.96NTFVLSAKK38 pKa = 9.98PNPLTEE44 pKa = 4.26NGRR47 pKa = 11.84QDD49 pKa = 3.08GWEE52 pKa = 3.79LRR54 pKa = 11.84IATVYY59 pKa = 10.97SVFDD63 pKa = 4.22ADD65 pKa = 6.07LDD67 pKa = 3.85AQQYY71 pKa = 7.97VIWCSSVDD79 pKa = 3.65TSTMDD84 pKa = 2.88ITIDD88 pKa = 3.67DD89 pKa = 4.26EE90 pKa = 4.61PYY92 pKa = 10.95EE93 pKa = 5.42DD94 pKa = 5.43GICDD98 pKa = 3.78TLEE101 pKa = 3.72EE102 pKa = 4.71AIKK105 pKa = 10.31YY106 pKa = 8.76LHH108 pKa = 6.88EE109 pKa = 4.09NTEE112 pKa = 3.79AFKK115 pKa = 10.53IYY117 pKa = 10.91

Molecular weight: 13.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345AV54|A0A345AV54_9CAUD RNA ligase 1 OS=Acinetobacter phage vB_ApiM_fHyAci03 OX=2269366 GN=rnlA PE=3 SV=1
MM1 pKa = 7.51LGLVILLVVALIFYY15 pKa = 7.32MKK17 pKa = 10.22RR18 pKa = 11.84VNRR21 pKa = 11.84QRR23 pKa = 11.84ALIRR27 pKa = 11.84KK28 pKa = 8.36LKK30 pKa = 11.0YY31 pKa = 9.98EE32 pKa = 4.21VFCKK36 pKa = 10.09TMEE39 pKa = 4.67FYY41 pKa = 10.61RR42 pKa = 11.84VSNEE46 pKa = 3.38LNRR49 pKa = 11.84IKK51 pKa = 10.89NPLPIIRR58 pKa = 11.84QCKK61 pKa = 7.57NLKK64 pKa = 10.29FNGFEE69 pKa = 4.13KK70 pKa = 10.29IAINFWIKK78 pKa = 10.77

Molecular weight: 9.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

247

0

247

51599

39

1314

208.9

23.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.971 ± 0.15

1.097 ± 0.072

6.2 ± 0.125

6.882 ± 0.184

4.339 ± 0.113

5.884 ± 0.19

1.959 ± 0.083

7.33 ± 0.11

7.13 ± 0.192

7.87 ± 0.153

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.63 ± 0.087

5.806 ± 0.124

3.607 ± 0.106

3.485 ± 0.098

4.31 ± 0.077

6.293 ± 0.171

6.14 ± 0.255

6.516 ± 0.11

1.322 ± 0.055

4.231 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski