Erwinia phage vB_EhrS_59

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Feofaniavirus; Erwinia virus Eho59

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NS98|A0A4Y1NS98_9CAUD Integrase OS=Erwinia phage vB_EhrS_59 OX=2283025 GN=MZUP2_280 PE=4 SV=1
MM1 pKa = 7.4SKK3 pKa = 10.64NYY5 pKa = 10.51ADD7 pKa = 3.62WNITLNTTCPEE18 pKa = 3.94CEE20 pKa = 4.13EE21 pKa = 4.16YY22 pKa = 10.74FDD24 pKa = 6.61LIEE27 pKa = 4.48QDD29 pKa = 3.13DD30 pKa = 4.54DD31 pKa = 3.99FWVDD35 pKa = 3.04GGRR38 pKa = 11.84AEE40 pKa = 4.14MCEE43 pKa = 4.06HH44 pKa = 6.64DD45 pKa = 3.64THH47 pKa = 6.02RR48 pKa = 11.84TKK50 pKa = 10.96DD51 pKa = 3.44VDD53 pKa = 4.17VEE55 pKa = 4.29CPEE58 pKa = 4.74CGHH61 pKa = 5.77EE62 pKa = 4.23FKK64 pKa = 11.23VDD66 pKa = 3.64FAYY69 pKa = 10.51

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NTA5|A0A4Y1NTA5_9CAUD Uncharacterized protein OS=Erwinia phage vB_EhrS_59 OX=2283025 GN=MZUP2_720 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.11PIDD5 pKa = 3.8HH6 pKa = 6.68YY7 pKa = 11.28RR8 pKa = 11.84LAMYY12 pKa = 9.65LQTRR16 pKa = 11.84KK17 pKa = 9.33QLEE20 pKa = 3.96NRR22 pKa = 11.84LAEE25 pKa = 4.23VNSKK29 pKa = 9.94IEE31 pKa = 3.88RR32 pKa = 11.84VQYY35 pKa = 9.35QSRR38 pKa = 11.84SRR40 pKa = 11.84KK41 pKa = 9.25PLGQRR46 pKa = 11.84ILNWWFAA53 pKa = 3.71

Molecular weight: 6.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

14286

49

1058

178.6

19.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.828 ± 0.7

1.4 ± 0.217

6.251 ± 0.252

6.279 ± 0.362

3.619 ± 0.231

6.818 ± 0.256

1.757 ± 0.183

5.509 ± 0.218

5.873 ± 0.338

7.602 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.66 ± 0.145

4.319 ± 0.204

3.871 ± 0.292

4.41 ± 0.33

6.013 ± 0.308

6.811 ± 0.303

6.314 ± 0.328

5.901 ± 0.188

1.715 ± 0.133

3.052 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski