Legionella feeleii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W0TMF1|A0A0W0TMF1_9GAMM Mlc titration factor A OS=Legionella feeleii OX=453 GN=mtfA PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.11KK3 pKa = 10.46AIVILTCIFSLNCFASDD20 pKa = 3.22YY21 pKa = 10.51SYY23 pKa = 11.61DD24 pKa = 3.7VSGEE28 pKa = 4.08DD29 pKa = 3.52EE30 pKa = 4.2NGKK33 pKa = 8.86EE34 pKa = 4.15LEE36 pKa = 4.04GTIYY40 pKa = 10.69SNKK43 pKa = 9.9GEE45 pKa = 4.06QEE47 pKa = 4.15VTGEE51 pKa = 4.13LTDD54 pKa = 3.58EE55 pKa = 5.18DD56 pKa = 4.66GNSVEE61 pKa = 6.56FEE63 pKa = 4.34GQWDD67 pKa = 4.29GQGHH71 pKa = 6.36ISGEE75 pKa = 4.35TEE77 pKa = 4.16DD78 pKa = 4.54GVSVDD83 pKa = 5.25LYY85 pKa = 11.08TEE87 pKa = 4.08

Molecular weight: 9.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W0TZB2|A0A0W0TZB2_9GAMM Mannose-6-phosphate isomerase OS=Legionella feeleii OX=453 GN=manC PE=4 SV=1
MM1 pKa = 7.41PHH3 pKa = 6.65SFQITRR9 pKa = 11.84QCSTCSGTHH18 pKa = 6.39GEE20 pKa = 4.24NQKK23 pKa = 9.95SLRR26 pKa = 11.84YY27 pKa = 9.71SRR29 pKa = 11.84WALQEE34 pKa = 4.06LSIKK38 pKa = 8.99ATNPVSEE45 pKa = 4.39RR46 pKa = 11.84QTNLAIRR53 pKa = 11.84CNRR56 pKa = 11.84APLRR60 pKa = 11.84LKK62 pKa = 10.9FEE64 pKa = 4.56LPSFLRR70 pKa = 11.84QQKK73 pKa = 10.47LSS75 pKa = 3.45

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3273

0

3273

1025687

50

3007

313.4

35.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.342 ± 0.045

1.208 ± 0.014

4.76 ± 0.029

5.946 ± 0.046

4.432 ± 0.03

6.139 ± 0.037

2.438 ± 0.021

6.972 ± 0.034

5.942 ± 0.035

11.22 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.019

4.555 ± 0.03

4.182 ± 0.027

4.745 ± 0.04

4.51 ± 0.029

6.32 ± 0.035

5.359 ± 0.03

6.091 ± 0.036

1.15 ± 0.015

3.362 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski