Alethinophid 1 reptarenavirus (isolate AlRrV1/Boa/USA/BC/2009) (Golden Gate virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Arenaviridae; Reptarenavirus; Golden reptarenavirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|J7HBH4|GLYC_GOGV Glycoprotein OS=Alethinophid 1 reptarenavirus (isolate AlRrV1/Boa/USA/BC/2009) OX=1223562 GN=GPC PE=3 SV=1
MM1 pKa = 7.28MSHH4 pKa = 6.62IRR6 pKa = 11.84LVLCSALLSMMSCPTSGTIGEE27 pKa = 4.46MLSLAASSNSSICHH41 pKa = 5.44GMQFTEE47 pKa = 4.24PTEE50 pKa = 4.25SFMGILPNEE59 pKa = 4.31TLPMFIFSIMHH70 pKa = 5.82SEE72 pKa = 4.09AVPKK76 pKa = 10.31VGKK79 pKa = 7.24TLRR82 pKa = 11.84ISHH85 pKa = 6.51DD86 pKa = 3.16MKK88 pKa = 11.12LFGGEE93 pKa = 3.91AVNYY97 pKa = 8.39MIFVNKK103 pKa = 9.91ILYY106 pKa = 9.87EE107 pKa = 4.43PISYY111 pKa = 10.83YY112 pKa = 10.95NYY114 pKa = 9.89TGSCRR119 pKa = 11.84QQGLSSCVRR128 pKa = 11.84VYY130 pKa = 11.48SDD132 pKa = 4.57LVNGSKK138 pKa = 10.89GNPSVKK144 pKa = 10.4LGFTAIEE151 pKa = 4.01LNKK154 pKa = 9.87TKK156 pKa = 9.72NTDD159 pKa = 3.77FHH161 pKa = 7.64NLGFKK166 pKa = 9.89ICFSCRR172 pKa = 11.84DD173 pKa = 3.51HH174 pKa = 8.05NGIRR178 pKa = 11.84LVVYY182 pKa = 10.09NKK184 pKa = 10.38NNRR187 pKa = 11.84TAQLMMCPSEE197 pKa = 5.57LIFSQDD203 pKa = 3.0FTINSTSVNPSEE215 pKa = 4.32EE216 pKa = 3.96AAVPLLNVTGYY227 pKa = 8.69TCIALHH233 pKa = 6.12NKK235 pKa = 9.58NMLTHH240 pKa = 6.87HH241 pKa = 6.48SPALSSGSKK250 pKa = 10.1VDD252 pKa = 3.46NTLEE256 pKa = 4.37PGCDD260 pKa = 3.31SNVGLFGHH268 pKa = 5.82STGTDD273 pKa = 3.69YY274 pKa = 11.67GWGLANFFSAGITNSLQISQLEE296 pKa = 4.34HH297 pKa = 5.01VTDD300 pKa = 6.18AIACKK305 pKa = 9.7IAKK308 pKa = 8.02TSNYY312 pKa = 6.3TTTALFLLNKK322 pKa = 10.02EE323 pKa = 3.94EE324 pKa = 4.5GEE326 pKa = 4.16IRR328 pKa = 11.84DD329 pKa = 3.66HH330 pKa = 7.37VIEE333 pKa = 5.1HH334 pKa = 6.04EE335 pKa = 4.28VALNYY340 pKa = 10.71LLAHH344 pKa = 5.98QGGLCSVVKK353 pKa = 10.85GPMCCSDD360 pKa = 2.91IDD362 pKa = 3.73DD363 pKa = 3.98FRR365 pKa = 11.84RR366 pKa = 11.84NVSDD370 pKa = 4.15MIDD373 pKa = 3.43KK374 pKa = 10.52VHH376 pKa = 6.86EE377 pKa = 4.18EE378 pKa = 3.85MKK380 pKa = 10.51KK381 pKa = 10.43FYY383 pKa = 10.66HH384 pKa = 6.44EE385 pKa = 4.57PDD387 pKa = 3.34PFGGLGTWGFYY398 pKa = 9.2GTIFGHH404 pKa = 5.09VLQWIPIIIMVVVVCFVCSWVRR426 pKa = 11.84KK427 pKa = 9.13

Molecular weight: 47.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|J7H5M4|NCAP_GOGV Nucleoprotein OS=Alethinophid 1 reptarenavirus (isolate AlRrV1/Boa/USA/BC/2009) OX=1223562 GN=N PE=3 SV=1
MM1 pKa = 7.4SGSTAIGLTTEE12 pKa = 4.4VISIITFILVIAIFVIEE29 pKa = 4.33IVSCVTMMTLKK40 pKa = 10.9AITLKK45 pKa = 10.64KK46 pKa = 10.26RR47 pKa = 11.84LSFCQGCGKK56 pKa = 9.8NASLVILPCKK66 pKa = 10.48NKK68 pKa = 9.76VCMEE72 pKa = 4.35CALKK76 pKa = 9.82MRR78 pKa = 11.84CPVCYY83 pKa = 9.02EE84 pKa = 3.65ACLWCEE90 pKa = 4.11NPDD93 pKa = 3.54GSLSSLALINKK104 pKa = 7.29EE105 pKa = 3.95RR106 pKa = 11.84NKK108 pKa = 9.9VRR110 pKa = 11.84DD111 pKa = 3.9NLPEE115 pKa = 3.89PP116 pKa = 4.2

Molecular weight: 12.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3200

116

2066

800.0

90.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.219 ± 1.098

2.406 ± 0.852

5.813 ± 0.845

7.187 ± 0.734

4.719 ± 0.235

5.375 ± 0.496

1.875 ± 0.467

6.688 ± 0.424

7.75 ± 0.75

11.25 ± 1.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.094 ± 0.306

4.906 ± 0.317

4.281 ± 0.619

2.656 ± 0.299

4.656 ± 0.552

7.938 ± 0.472

5.594 ± 0.284

5.844 ± 0.372

1.0 ± 0.194

2.75 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski