Xenopus laevis (African clawed frog)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea;

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44580 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L8GXJ2|A0A1L8GXJ2_XENLA Uncharacterized protein OS=Xenopus laevis OX=8355 GN=XELAEV_18017144mg PE=4 SV=1
MM1 pKa = 6.86VFGWALLQLPTGFPRR16 pKa = 11.84SEE18 pKa = 5.15DD19 pKa = 3.26IFPPCLDD26 pKa = 3.47MVEE29 pKa = 4.4VIIPRR34 pKa = 11.84GGDD37 pKa = 3.29LVVVLTGDD45 pKa = 3.55GFVVGEE51 pKa = 4.45GTLSLSFSFDD61 pKa = 2.4GGMFFITFDD70 pKa = 3.69NDD72 pKa = 3.28EE73 pKa = 5.28EE74 pKa = 4.1ILPWGFVSEE83 pKa = 4.38EE84 pKa = 4.05TFDD87 pKa = 3.89PDD89 pKa = 4.5KK90 pKa = 11.46AVLKK94 pKa = 10.54CIAFVGDD101 pKa = 3.38TAFLLFGNCKK111 pKa = 10.06AVFPACFGLWKK122 pKa = 9.38VTLPADD128 pKa = 3.95FWIDD132 pKa = 3.34KK133 pKa = 10.28LCFSTAFCFGEE144 pKa = 4.41TLLPPPFFFSVADD157 pKa = 3.68SSISFAVGYY166 pKa = 10.57KK167 pKa = 10.27SFLLFSFCVLTFPMHH182 pKa = 7.17FILDD186 pKa = 4.03TQVLDD191 pKa = 4.86LIFGTEE197 pKa = 3.99YY198 pKa = 11.08SVDD201 pKa = 3.47PP202 pKa = 4.55

Molecular weight: 22.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A310TM63|A0A310TM63_XENLA Ig-like domain-containing protein (Fragment) OS=Xenopus laevis OX=8355 GN=XELAEV_18000504mg PE=4 SV=1
MM1 pKa = 7.18SRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 7.17MSRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 7.6MSGRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 7.32MSRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84KK31 pKa = 7.96MSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 7.65MSRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 7.32MSRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84KK53 pKa = 8.13MSRR56 pKa = 11.84RR57 pKa = 11.84KK58 pKa = 9.03ISRR61 pKa = 11.84IRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84KK66 pKa = 7.17MSRR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84KK72 pKa = 7.36MRR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84KK77 pKa = 9.08LRR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84LLGRR87 pKa = 11.84YY88 pKa = 7.26SHH90 pKa = 6.56CTSSASSVKK99 pKa = 10.2RR100 pKa = 11.84IKK102 pKa = 10.5HH103 pKa = 5.08KK104 pKa = 10.67SAAVGNTEE112 pKa = 4.96PPITTHH118 pKa = 5.75KK119 pKa = 7.08TTPLLL124 pKa = 4.0

Molecular weight: 15.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43236

1344

44580

19963946

9

18453

447.8

50.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.871 ± 0.01

2.431 ± 0.013

4.947 ± 0.01

6.771 ± 0.018

3.864 ± 0.011

5.803 ± 0.016

2.659 ± 0.008

5.3 ± 0.012

6.242 ± 0.016

9.441 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.005

4.447 ± 0.008

5.408 ± 0.016

4.634 ± 0.015

5.081 ± 0.01

8.751 ± 0.019

5.794 ± 0.016

6.003 ± 0.011

1.158 ± 0.004

3.019 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski