Human immunodeficiency virus type 1 group M subtype B (isolate RF/HAT3) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 unknown group

Average proteome isoelectric point is 7.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P05908-2|TAT-2_HV1RH Isoform of P05908 Isoform Short of Protein Tat OS=Human immunodeficiency virus type 1 group M subtype B (isolate RF/HAT3) OX=11701 GN=tat PE=4 SV=1
MM1 pKa = 7.3QSLEE5 pKa = 3.75ILAIVALVVAAILAIVVWTIVGIEE29 pKa = 3.69IRR31 pKa = 11.84KK32 pKa = 7.19TLRR35 pKa = 11.84QKK37 pKa = 10.92KK38 pKa = 8.98IDD40 pKa = 3.72RR41 pKa = 11.84LIDD44 pKa = 4.37RR45 pKa = 11.84IRR47 pKa = 11.84EE48 pKa = 3.87RR49 pKa = 11.84AEE51 pKa = 4.03DD52 pKa = 3.71SGNEE56 pKa = 3.82SDD58 pKa = 4.89GDD60 pKa = 3.99EE61 pKa = 4.63EE62 pKa = 4.1EE63 pKa = 5.09LSALVEE69 pKa = 4.45MGHH72 pKa = 6.13HH73 pKa = 6.48APWDD77 pKa = 3.7VDD79 pKa = 3.84DD80 pKa = 4.78LL81 pKa = 4.6

Molecular weight: 9.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P05908|TAT_HV1RH Protein Tat OS=Human immunodeficiency virus type 1 group M subtype B (isolate RF/HAT3) OX=11701 GN=tat PE=3 SV=1
MM1 pKa = 7.3EE2 pKa = 5.21NRR4 pKa = 11.84WQVMIVWQVDD14 pKa = 3.04RR15 pKa = 11.84MRR17 pKa = 11.84IRR19 pKa = 11.84TWKK22 pKa = 10.59SLVKK26 pKa = 9.27HH27 pKa = 6.05HH28 pKa = 6.44MYY30 pKa = 10.4ISRR33 pKa = 11.84KK34 pKa = 8.68AKK36 pKa = 8.16GWFYY40 pKa = 9.78RR41 pKa = 11.84HH42 pKa = 6.5HH43 pKa = 7.05YY44 pKa = 9.94EE45 pKa = 3.98STHH48 pKa = 5.61PRR50 pKa = 11.84ISSEE54 pKa = 3.87VHH56 pKa = 5.75IPPGDD61 pKa = 3.49EE62 pKa = 3.39RR63 pKa = 11.84LVITTYY69 pKa = 9.92WGLHH73 pKa = 4.28TGEE76 pKa = 4.83RR77 pKa = 11.84DD78 pKa = 2.76WHH80 pKa = 6.32LGQGVSIEE88 pKa = 3.9WRR90 pKa = 11.84KK91 pKa = 9.69RR92 pKa = 11.84RR93 pKa = 11.84YY94 pKa = 8.15STQVDD99 pKa = 3.79PDD101 pKa = 3.78LADD104 pKa = 3.59QLIHH108 pKa = 7.18LYY110 pKa = 10.94YY111 pKa = 10.17FDD113 pKa = 5.17CFSEE117 pKa = 4.33SAIRR121 pKa = 11.84KK122 pKa = 7.99PSLGHH127 pKa = 5.83IVSPRR132 pKa = 11.84CEE134 pKa = 3.98YY135 pKa = 10.43QAGHH139 pKa = 6.19NKK141 pKa = 9.66VGSLQYY147 pKa = 10.68LALAALTTPKK157 pKa = 10.16KK158 pKa = 10.06IKK160 pKa = 10.2PPLPSVKK167 pKa = 10.27KK168 pKa = 9.35LTEE171 pKa = 4.22DD172 pKa = 3.5RR173 pKa = 11.84WNKK176 pKa = 7.33PQKK179 pKa = 9.13TKK181 pKa = 8.84GHH183 pKa = 6.53RR184 pKa = 11.84GSHH187 pKa = 4.81TMNGHH192 pKa = 6.05

Molecular weight: 22.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3669

72

1436

366.9

41.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.269 ± 0.525

2.071 ± 0.458

4.088 ± 0.461

6.95 ± 0.49

2.453 ± 0.191

7.304 ± 0.113

2.371 ± 0.478

6.214 ± 0.816

7.386 ± 0.931

8.258 ± 0.507

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.153 ± 0.178

4.524 ± 0.921

5.26 ± 0.696

6.051 ± 0.444

5.833 ± 0.794

5.342 ± 0.525

5.914 ± 0.499

6.296 ± 0.414

2.698 ± 0.246

2.562 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski