Streptomyces rubellomurinus (strain ATCC 31215)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F2TAJ6|A0A0F2TAJ6_STRR3 DNA polymerase III subunit epsilon OS=Streptomyces rubellomurinus (strain ATCC 31215) OX=359131 GN=VM95_27805 PE=4 SV=1
MM1 pKa = 7.49PVVTDD6 pKa = 4.33PYY8 pKa = 11.32KK9 pKa = 10.75PGTPCWIDD17 pKa = 4.43LMASDD22 pKa = 4.32QQAALDD28 pKa = 4.19FYY30 pKa = 11.32RR31 pKa = 11.84DD32 pKa = 3.38LFGWQGEE39 pKa = 4.19AGPAEE44 pKa = 4.06FGGYY48 pKa = 8.98AVCTLNGRR56 pKa = 11.84PVAGIMAQSAPEE68 pKa = 4.22GQPLPPVAWTTYY80 pKa = 9.83LASDD84 pKa = 4.38DD85 pKa = 4.81ADD87 pKa = 3.17ATMRR91 pKa = 11.84AVTEE95 pKa = 4.06AGGTVLYY102 pKa = 10.9EE103 pKa = 4.15PMDD106 pKa = 3.49VGAIGRR112 pKa = 11.84MLVAADD118 pKa = 3.31PTGAVFGVWQAMDD131 pKa = 4.24FIGAGVVNEE140 pKa = 4.37PGALVWNEE148 pKa = 4.09LNTSDD153 pKa = 3.92TEE155 pKa = 4.08AAGRR159 pKa = 11.84FYY161 pKa = 11.03HH162 pKa = 6.72PALGLRR168 pKa = 11.84PATIQGMDD176 pKa = 3.43GYY178 pKa = 11.37YY179 pKa = 10.32SLNVGDD185 pKa = 3.86RR186 pKa = 11.84TVGGMQAIPGYY197 pKa = 10.54LSGDD201 pKa = 4.01TPSHH205 pKa = 5.18WMTYY209 pKa = 10.06FSVDD213 pKa = 3.75DD214 pKa = 4.49ADD216 pKa = 3.98STVDD220 pKa = 3.6ALVKK224 pKa = 10.78AGGSVIQPPFDD235 pKa = 3.62MQSGRR240 pKa = 11.84MAVVRR245 pKa = 11.84DD246 pKa = 3.62PQGAAFAVIQAPEE259 pKa = 3.94AQLPDD264 pKa = 3.68SPP266 pKa = 4.86

Molecular weight: 27.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F2TEK6|A0A0F2TEK6_STRR3 Sugar transporter OS=Streptomyces rubellomurinus (strain ATCC 31215) OX=359131 GN=VM95_22770 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.43GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6267

0

6267

1971839

29

3868

314.6

33.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.582 ± 0.06

0.786 ± 0.01

5.877 ± 0.022

5.679 ± 0.033

2.677 ± 0.018

9.583 ± 0.031

2.303 ± 0.013

2.923 ± 0.024

1.912 ± 0.025

10.836 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.567 ± 0.013

1.717 ± 0.019

6.278 ± 0.031

2.821 ± 0.017

8.011 ± 0.036

4.649 ± 0.027

5.906 ± 0.029

8.403 ± 0.028

1.501 ± 0.013

1.991 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski