Amino acid dipepetide frequency for Nocardioides sp. Root614

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.704AlaAla: 18.704 ± 0.161
0.998AlaCys: 0.998 ± 0.027
8.522AlaAsp: 8.522 ± 0.08
7.795AlaGlu: 7.795 ± 0.105
3.64AlaPhe: 3.64 ± 0.055
11.874AlaGly: 11.874 ± 0.094
2.478AlaHis: 2.478 ± 0.043
5.133AlaIle: 5.133 ± 0.057
2.831AlaLys: 2.831 ± 0.055
13.091AlaLeu: 13.091 ± 0.111
2.725AlaMet: 2.725 ± 0.045
2.337AlaAsn: 2.337 ± 0.055
6.099AlaPro: 6.099 ± 0.082
3.474AlaGln: 3.474 ± 0.055
8.583AlaArg: 8.583 ± 0.111
6.684AlaSer: 6.684 ± 0.072
7.939AlaThr: 7.939 ± 0.081
11.471AlaVal: 11.471 ± 0.11
1.894AlaTrp: 1.894 ± 0.037
2.372AlaTyr: 2.372 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.943CysAla: 0.943 ± 0.022
0.084CysCys: 0.084 ± 0.007
0.468CysAsp: 0.468 ± 0.02
0.38CysGlu: 0.38 ± 0.015
0.225CysPhe: 0.225 ± 0.012
0.84CysGly: 0.84 ± 0.025
0.168CysHis: 0.168 ± 0.011
0.238CysIle: 0.238 ± 0.013
0.125CysLys: 0.125 ± 0.009
0.654CysLeu: 0.654 ± 0.02
0.118CysMet: 0.118 ± 0.007
0.17CysAsn: 0.17 ± 0.011
0.472CysPro: 0.472 ± 0.02
0.2CysGln: 0.2 ± 0.011
0.529CysArg: 0.529 ± 0.018
0.463CysSer: 0.463 ± 0.021
0.503CysThr: 0.503 ± 0.023
0.613CysVal: 0.613 ± 0.02
0.129CysTrp: 0.129 ± 0.016
0.151CysTyr: 0.151 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
8.075AspAla: 8.075 ± 0.084
0.442AspCys: 0.442 ± 0.017
4.774AspAsp: 4.774 ± 0.076
4.344AspGlu: 4.344 ± 0.064
1.799AspPhe: 1.799 ± 0.036
6.586AspGly: 6.586 ± 0.081
1.544AspHis: 1.544 ± 0.031
2.246AspIle: 2.246 ± 0.04
1.339AspLys: 1.339 ± 0.033
7.561AspLeu: 7.561 ± 0.077
0.834AspMet: 0.834 ± 0.023
1.202AspAsn: 1.202 ± 0.035
4.594AspPro: 4.594 ± 0.063
1.941AspGln: 1.941 ± 0.039
4.773AspArg: 4.773 ± 0.063
2.672AspSer: 2.672 ± 0.047
3.003AspThr: 3.003 ± 0.045
6.132AspVal: 6.132 ± 0.078
0.996AspTrp: 0.996 ± 0.027
1.197AspTyr: 1.197 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
6.881GluAla: 6.881 ± 0.089
0.352GluCys: 0.352 ± 0.014
3.036GluAsp: 3.036 ± 0.051
3.2GluGlu: 3.2 ± 0.058
1.659GluPhe: 1.659 ± 0.034
4.201GluGly: 4.201 ± 0.052
1.515GluHis: 1.515 ± 0.037
2.717GluIle: 2.717 ± 0.042
1.516GluLys: 1.516 ± 0.037
6.279GluLeu: 6.279 ± 0.077
1.022GluMet: 1.022 ± 0.027
1.116GluAsn: 1.116 ± 0.033
3.085GluPro: 3.085 ± 0.051
2.262GluGln: 2.262 ± 0.044
4.696GluArg: 4.696 ± 0.061
2.762GluSer: 2.762 ± 0.041
2.956GluThr: 2.956 ± 0.05
5.294GluVal: 5.294 ± 0.059
0.845GluTrp: 0.845 ± 0.023
1.075GluTyr: 1.075 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
3.808PheAla: 3.808 ± 0.052
0.264PheCys: 0.264 ± 0.012
2.242PheAsp: 2.242 ± 0.041
1.74PheGlu: 1.74 ± 0.038
0.929PhePhe: 0.929 ± 0.029
3.168PheGly: 3.168 ± 0.058
0.639PheHis: 0.639 ± 0.02
0.927PheIle: 0.927 ± 0.023
0.592PheLys: 0.592 ± 0.02
2.632PheLeu: 2.632 ± 0.043
0.428PheMet: 0.428 ± 0.018
0.692PheAsn: 0.692 ± 0.023
1.36PhePro: 1.36 ± 0.03
0.72PheGln: 0.72 ± 0.021
1.783PheArg: 1.783 ± 0.034
1.652PheSer: 1.652 ± 0.033
2.044PheThr: 2.044 ± 0.04
2.58PheVal: 2.58 ± 0.038
0.395PheTrp: 0.395 ± 0.019
0.633PheTyr: 0.633 ± 0.023
0.0PheXaa: 0.0 ± 0.0
Gly
10.233GlyAla: 10.233 ± 0.074
0.79GlyCys: 0.79 ± 0.021
5.752GlyAsp: 5.752 ± 0.074
4.874GlyGlu: 4.874 ± 0.057
3.038GlyPhe: 3.038 ± 0.045
8.157GlyGly: 8.157 ± 0.093
1.99GlyHis: 1.99 ± 0.031
4.004GlyIle: 4.004 ± 0.053
2.496GlyLys: 2.496 ± 0.047
9.254GlyLeu: 9.254 ± 0.088
1.81GlyMet: 1.81 ± 0.039
2.058GlyAsn: 2.058 ± 0.046
4.438GlyPro: 4.438 ± 0.058
2.761GlyGln: 2.761 ± 0.04
6.545GlyArg: 6.545 ± 0.074
5.499GlySer: 5.499 ± 0.067
5.918GlyThr: 5.918 ± 0.088
7.902GlyVal: 7.902 ± 0.078
1.639GlyTrp: 1.639 ± 0.032
2.171GlyTyr: 2.171 ± 0.042
0.001GlyXaa: 0.001 ± 0.001
His
2.406HisAla: 2.406 ± 0.041
0.19HisCys: 0.19 ± 0.01
1.412HisAsp: 1.412 ± 0.03
1.173HisGlu: 1.173 ± 0.028
0.671HisPhe: 0.671 ± 0.021
2.125HisGly: 2.125 ± 0.038
0.677HisHis: 0.677 ± 0.024
0.574HisIle: 0.574 ± 0.02
0.363HisLys: 0.363 ± 0.016
2.324HisLeu: 2.324 ± 0.045
0.278HisMet: 0.278 ± 0.014
0.385HisAsn: 0.385 ± 0.018
1.517HisPro: 1.517 ± 0.029
0.671HisGln: 0.671 ± 0.021
1.765HisArg: 1.765 ± 0.037
0.903HisSer: 0.903 ± 0.025
1.113HisThr: 1.113 ± 0.031
1.868HisVal: 1.868 ± 0.036
0.311HisTrp: 0.311 ± 0.015
0.482HisTyr: 0.482 ± 0.019
0.001HisXaa: 0.001 ± 0.001
Ile
5.769IleAla: 5.769 ± 0.07
0.319IleCys: 0.319 ± 0.015
3.273IleAsp: 3.273 ± 0.045
2.67IleGlu: 2.67 ± 0.046
0.921IlePhe: 0.921 ± 0.027
4.281IleGly: 4.281 ± 0.058
0.754IleHis: 0.754 ± 0.021
1.244IleIle: 1.244 ± 0.028
0.968IleLys: 0.968 ± 0.025
2.808IleLeu: 2.808 ± 0.047
0.491IleMet: 0.491 ± 0.018
1.047IleAsn: 1.047 ± 0.03
2.021IlePro: 2.021 ± 0.039
0.912IleGln: 0.912 ± 0.026
2.521IleArg: 2.521 ± 0.045
2.199IleSer: 2.199 ± 0.043
2.649IleThr: 2.649 ± 0.043
3.501IleVal: 3.501 ± 0.055
0.437IleTrp: 0.437 ± 0.018
0.688IleTyr: 0.688 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
3.034LysAla: 3.034 ± 0.055
0.117LysCys: 0.117 ± 0.008
1.373LysAsp: 1.373 ± 0.035
1.153LysGlu: 1.153 ± 0.027
0.598LysPhe: 0.598 ± 0.022
1.802LysGly: 1.802 ± 0.044
0.48LysHis: 0.48 ± 0.019
1.036LysIle: 1.036 ± 0.034
0.807LysLys: 0.807 ± 0.033
2.014LysLeu: 2.014 ± 0.04
0.451LysMet: 0.451 ± 0.018
0.571LysAsn: 0.571 ± 0.025
1.345LysPro: 1.345 ± 0.032
0.778LysGln: 0.778 ± 0.025
1.504LysArg: 1.504 ± 0.033
1.26LysSer: 1.26 ± 0.031
1.284LysThr: 1.284 ± 0.03
2.314LysVal: 2.314 ± 0.042
0.272LysTrp: 0.272 ± 0.013
0.53LysTyr: 0.53 ± 0.019
0.001LysXaa: 0.001 ± 0.001
Leu
14.484LeuAla: 14.484 ± 0.131
0.718LeuCys: 0.718 ± 0.022
6.999LeuAsp: 6.999 ± 0.079
5.37LeuGlu: 5.37 ± 0.069
2.534LeuPhe: 2.534 ± 0.045
9.314LeuGly: 9.314 ± 0.096
2.031LeuHis: 2.031 ± 0.039
3.446LeuIle: 3.446 ± 0.051
2.067LeuLys: 2.067 ± 0.036
10.318LeuLeu: 10.318 ± 0.134
1.617LeuMet: 1.617 ± 0.036
1.886LeuAsn: 1.886 ± 0.042
5.573LeuPro: 5.573 ± 0.062
2.458LeuGln: 2.458 ± 0.045
7.299LeuArg: 7.299 ± 0.083
5.429LeuSer: 5.429 ± 0.063
6.522LeuThr: 6.522 ± 0.07
9.832LeuVal: 9.832 ± 0.085
1.179LeuTrp: 1.179 ± 0.03
1.513LeuTyr: 1.513 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
2.362MetAla: 2.362 ± 0.038
0.134MetCys: 0.134 ± 0.009
0.859MetAsp: 0.859 ± 0.023
0.735MetGlu: 0.735 ± 0.022
0.503MetPhe: 0.503 ± 0.02
1.342MetGly: 1.342 ± 0.031
0.357MetHis: 0.357 ± 0.015
0.725MetIle: 0.725 ± 0.023
0.506MetLys: 0.506 ± 0.019
1.811MetLeu: 1.811 ± 0.031
0.347MetMet: 0.347 ± 0.016
0.439MetAsn: 0.439 ± 0.02
1.039MetPro: 1.039 ± 0.025
0.474MetGln: 0.474 ± 0.019
1.308MetArg: 1.308 ± 0.03
1.442MetSer: 1.442 ± 0.031
1.676MetThr: 1.676 ± 0.034
1.545MetVal: 1.545 ± 0.033
0.195MetTrp: 0.195 ± 0.011
0.304MetTyr: 0.304 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.496AsnAla: 2.496 ± 0.048
0.179AsnCys: 0.179 ± 0.011
1.361AsnAsp: 1.361 ± 0.031
1.053AsnGlu: 1.053 ± 0.028
0.572AsnPhe: 0.572 ± 0.019
2.086AsnGly: 2.086 ± 0.051
0.431AsnHis: 0.431 ± 0.016
0.785AsnIle: 0.785 ± 0.025
0.532AsnLys: 0.532 ± 0.024
2.05AsnLeu: 2.05 ± 0.043
0.32AsnMet: 0.32 ± 0.015
0.537AsnAsn: 0.537 ± 0.024
1.554AsnPro: 1.554 ± 0.033
0.655AsnGln: 0.655 ± 0.02
1.352AsnArg: 1.352 ± 0.029
0.947AsnSer: 0.947 ± 0.034
1.24AsnThr: 1.24 ± 0.038
1.728AsnVal: 1.728 ± 0.038
0.304AsnTrp: 0.304 ± 0.013
0.477AsnTyr: 0.477 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
7.098ProAla: 7.098 ± 0.091
0.321ProCys: 0.321 ± 0.015
4.346ProAsp: 4.346 ± 0.054
3.758ProGlu: 3.758 ± 0.056
1.562ProPhe: 1.562 ± 0.032
5.468ProGly: 5.468 ± 0.063
1.078ProHis: 1.078 ± 0.026
1.975ProIle: 1.975 ± 0.038
1.195ProLys: 1.195 ± 0.032
4.76ProLeu: 4.76 ± 0.055
1.034ProMet: 1.034 ± 0.025
1.075ProAsn: 1.075 ± 0.03
2.711ProPro: 2.711 ± 0.058
1.397ProGln: 1.397 ± 0.03
3.22ProArg: 3.22 ± 0.053
3.109ProSer: 3.109 ± 0.05
3.569ProThr: 3.569 ± 0.06
5.199ProVal: 5.199 ± 0.072
0.878ProTrp: 0.878 ± 0.024
1.151ProTyr: 1.151 ± 0.027
0.001ProXaa: 0.001 ± 0.001
Gln
3.533GlnAla: 3.533 ± 0.053
0.166GlnCys: 0.166 ± 0.012
1.349GlnAsp: 1.349 ± 0.031
1.276GlnGlu: 1.276 ± 0.032
0.85GlnPhe: 0.85 ± 0.026
2.135GlnGly: 2.135 ± 0.038
0.665GlnHis: 0.665 ± 0.02
1.274GlnIle: 1.274 ± 0.026
0.634GlnLys: 0.634 ± 0.021
3.098GlnLeu: 3.098 ± 0.046
0.601GlnMet: 0.601 ± 0.019
0.517GlnAsn: 0.517 ± 0.02
1.661GlnPro: 1.661 ± 0.036
1.157GlnGln: 1.157 ± 0.028
2.365GlnArg: 2.365 ± 0.047
1.324GlnSer: 1.324 ± 0.028
1.531GlnThr: 1.531 ± 0.032
2.995GlnVal: 2.995 ± 0.045
0.461GlnTrp: 0.461 ± 0.018
0.565GlnTyr: 0.565 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
8.458ArgAla: 8.458 ± 0.106
0.463ArgCys: 0.463 ± 0.022
4.26ArgAsp: 4.26 ± 0.059
4.036ArgGlu: 4.036 ± 0.053
2.307ArgPhe: 2.307 ± 0.035
5.223ArgGly: 5.223 ± 0.068
1.653ArgHis: 1.653 ± 0.038
3.381ArgIle: 3.381 ± 0.046
1.655ArgLys: 1.655 ± 0.038
7.44ArgLeu: 7.44 ± 0.089
1.646ArgMet: 1.646 ± 0.032
1.468ArgAsn: 1.468 ± 0.032
3.77ArgPro: 3.77 ± 0.057
2.065ArgGln: 2.065 ± 0.043
6.196ArgArg: 6.196 ± 0.087
4.106ArgSer: 4.106 ± 0.057
4.427ArgThr: 4.427 ± 0.054
5.682ArgVal: 5.682 ± 0.053
1.297ArgTrp: 1.297 ± 0.031
1.529ArgTyr: 1.529 ± 0.033
0.001ArgXaa: 0.001 ± 0.001
Ser
6.532SerAla: 6.532 ± 0.066
0.423SerCys: 0.423 ± 0.018
3.273SerAsp: 3.273 ± 0.053
2.612SerGlu: 2.612 ± 0.048
1.731SerPhe: 1.731 ± 0.035
5.933SerGly: 5.933 ± 0.077
1.032SerHis: 1.032 ± 0.026
2.16SerIle: 2.16 ± 0.038
1.168SerLys: 1.168 ± 0.028
5.102SerLeu: 5.102 ± 0.059
1.241SerMet: 1.241 ± 0.026
1.115SerAsn: 1.115 ± 0.032
3.049SerPro: 3.049 ± 0.047
1.372SerGln: 1.372 ± 0.027
3.678SerArg: 3.678 ± 0.053
3.375SerSer: 3.375 ± 0.06
3.739SerThr: 3.739 ± 0.053
4.582SerVal: 4.582 ± 0.059
0.878SerTrp: 0.878 ± 0.026
1.491SerTyr: 1.491 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
7.785ThrAla: 7.785 ± 0.086
0.532ThrCys: 0.532 ± 0.021
3.946ThrAsp: 3.946 ± 0.063
3.195ThrGlu: 3.195 ± 0.048
2.04ThrPhe: 2.04 ± 0.033
6.198ThrGly: 6.198 ± 0.084
1.193ThrHis: 1.193 ± 0.026
2.614ThrIle: 2.614 ± 0.048
1.325ThrLys: 1.325 ± 0.029
5.782ThrLeu: 5.782 ± 0.064
1.088ThrMet: 1.088 ± 0.026
1.335ThrAsn: 1.335 ± 0.038
3.836ThrPro: 3.836 ± 0.053
1.46ThrGln: 1.46 ± 0.027
3.777ThrArg: 3.777 ± 0.054
3.804ThrSer: 3.804 ± 0.057
4.372ThrThr: 4.372 ± 0.074
5.859ThrVal: 5.859 ± 0.074
1.027ThrTrp: 1.027 ± 0.025
1.452ThrTyr: 1.452 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
12.112ValAla: 12.112 ± 0.108
0.67ValCys: 0.67 ± 0.022
6.087ValAsp: 6.087 ± 0.076
5.417ValGlu: 5.417 ± 0.067
2.394ValPhe: 2.394 ± 0.041
7.485ValGly: 7.485 ± 0.07
1.843ValHis: 1.843 ± 0.035
3.835ValIle: 3.835 ± 0.048
1.884ValLys: 1.884 ± 0.037
9.749ValLeu: 9.749 ± 0.096
1.531ValMet: 1.531 ± 0.031
1.923ValAsn: 1.923 ± 0.038
5.12ValPro: 5.12 ± 0.062
2.299ValGln: 2.299 ± 0.044
6.508ValArg: 6.508 ± 0.071
4.775ValSer: 4.775 ± 0.06
6.052ValThr: 6.052 ± 0.083
9.667ValVal: 9.667 ± 0.113
1.107ValTrp: 1.107 ± 0.028
1.456ValTyr: 1.456 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.564TrpAla: 1.564 ± 0.034
0.133TrpCys: 0.133 ± 0.009
0.837TrpAsp: 0.837 ± 0.021
0.685TrpGlu: 0.685 ± 0.023
0.537TrpPhe: 0.537 ± 0.019
1.072TrpGly: 1.072 ± 0.03
0.349TrpHis: 0.349 ± 0.013
0.655TrpIle: 0.655 ± 0.021
0.35TrpLys: 0.35 ± 0.016
1.751TrpLeu: 1.751 ± 0.038
0.305TrpMet: 0.305 ± 0.014
0.387TrpAsn: 0.387 ± 0.015
0.695TrpPro: 0.695 ± 0.023
0.544TrpGln: 0.544 ± 0.018
1.191TrpArg: 1.191 ± 0.029
1.028TrpSer: 1.028 ± 0.027
0.997TrpThr: 0.997 ± 0.03
1.172TrpVal: 1.172 ± 0.03
0.347TrpTrp: 0.347 ± 0.016
0.305TrpTyr: 0.305 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.363TyrAla: 2.363 ± 0.041
0.179TyrCys: 0.179 ± 0.01
1.885TyrAsp: 1.885 ± 0.037
1.001TyrGlu: 1.001 ± 0.026
0.718TyrPhe: 0.718 ± 0.023
2.036TyrGly: 2.036 ± 0.038
0.331TyrHis: 0.331 ± 0.015
0.503TyrIle: 0.503 ± 0.017
0.371TyrLys: 0.371 ± 0.016
2.112TyrLeu: 2.112 ± 0.041
0.218TyrMet: 0.218 ± 0.011
0.398TyrAsn: 0.398 ± 0.016
0.967TyrPro: 0.967 ± 0.03
0.556TyrGln: 0.556 ± 0.018
1.471TyrArg: 1.471 ± 0.032
1.055TyrSer: 1.055 ± 0.026
1.082TyrThr: 1.082 ± 0.029
1.979TyrVal: 1.979 ± 0.039
0.314TyrTrp: 0.314 ± 0.015
0.457TyrTyr: 0.457 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.001XaaAla: 0.001 ± 0.001
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.001XaaPro: 0.001 ± 0.001
0.0XaaGln: 0.0 ± 0.0
0.001XaaArg: 0.001 ± 0.001
0.001XaaSer: 0.001 ± 0.001
0.001XaaThr: 0.001 ± 0.001
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4780 proteins (1577883 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski