McMurdo Ice Shelf pond-associated circular DNA virus-3

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075M3P7|A0A075M3P7_9VIRU Putative capsid protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-3 OX=1521387 PE=4 SV=1
MM1 pKa = 7.4SKK3 pKa = 10.19NRR5 pKa = 11.84NFVFTWNNYY14 pKa = 8.55SDD16 pKa = 3.72ASKK19 pKa = 9.8TYY21 pKa = 10.82LSTLACKK28 pKa = 9.67YY29 pKa = 9.19VAYY32 pKa = 10.13AEE34 pKa = 4.49EE35 pKa = 4.31VAPTTGTRR43 pKa = 11.84HH44 pKa = 5.11LQGFIAFTNAKK55 pKa = 8.95TIQQARR61 pKa = 11.84SKK63 pKa = 11.15LPGCHH68 pKa = 5.89VEE70 pKa = 4.29TMNGSIAQSEE80 pKa = 4.98DD81 pKa = 3.59YY82 pKa = 10.51CSKK85 pKa = 11.06AGTLTEE91 pKa = 5.1HH92 pKa = 5.6GTKK95 pKa = 10.26PISNDD100 pKa = 2.95NNGRR104 pKa = 11.84AEE106 pKa = 3.91KK107 pKa = 10.65LRR109 pKa = 11.84WQRR112 pKa = 11.84ARR114 pKa = 11.84DD115 pKa = 3.52FAKK118 pKa = 10.36EE119 pKa = 3.97GKK121 pKa = 9.93LDD123 pKa = 4.8EE124 pKa = 5.0IDD126 pKa = 3.3ADD128 pKa = 3.65IFIRR132 pKa = 11.84CYY134 pKa = 9.92STLKK138 pKa = 10.39RR139 pKa = 11.84IKK141 pKa = 10.01SDD143 pKa = 3.5YY144 pKa = 8.51ATKK147 pKa = 9.54PQPIDD152 pKa = 3.61PVCIWIYY159 pKa = 11.03GPTGTGKK166 pKa = 9.45SHH168 pKa = 6.59AVEE171 pKa = 4.22TRR173 pKa = 11.84FPNCYY178 pKa = 9.04KK179 pKa = 10.8KK180 pKa = 11.11CMDD183 pKa = 4.11DD184 pKa = 4.98LKK186 pKa = 10.92WFDD189 pKa = 4.09GYY191 pKa = 11.52AHH193 pKa = 7.43EE194 pKa = 4.76DD195 pKa = 3.41AIYY198 pKa = 10.97LEE200 pKa = 6.04DD201 pKa = 3.52IDD203 pKa = 5.02KK204 pKa = 11.27YY205 pKa = 7.36QVKK208 pKa = 9.58WGGILKK214 pKa = 10.22RR215 pKa = 11.84LADD218 pKa = 4.59RR219 pKa = 11.84WPMQASIKK227 pKa = 10.64GAMAYY232 pKa = 9.19IRR234 pKa = 11.84PKK236 pKa = 10.48FVLVTSNYY244 pKa = 10.19RR245 pKa = 11.84IDD247 pKa = 5.29EE248 pKa = 4.17IWTDD252 pKa = 3.79PQTLEE257 pKa = 3.55PLQRR261 pKa = 11.84RR262 pKa = 11.84FTEE265 pKa = 4.13IEE267 pKa = 4.16KK268 pKa = 9.18LTQEE272 pKa = 3.96QVIDD276 pKa = 4.19FDD278 pKa = 3.77QQ279 pKa = 4.78

Molecular weight: 32.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075M3P7|A0A075M3P7_9VIRU Putative capsid protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-3 OX=1521387 PE=4 SV=1
MM1 pKa = 7.99PNRR4 pKa = 11.84KK5 pKa = 8.46VVKK8 pKa = 9.24KK9 pKa = 10.08SKK11 pKa = 10.03RR12 pKa = 11.84KK13 pKa = 8.46RR14 pKa = 11.84TFGQKK19 pKa = 9.03GLRR22 pKa = 11.84MLSQAAGAAVGYY34 pKa = 7.6GTHH37 pKa = 6.42NLAGIAGGAEE47 pKa = 4.0VGGYY51 pKa = 10.38LYY53 pKa = 11.17DD54 pKa = 3.81KK55 pKa = 10.24MLGEE59 pKa = 5.28AYY61 pKa = 10.28DD62 pKa = 3.91DD63 pKa = 4.1LGRR66 pKa = 11.84DD67 pKa = 3.54AMVEE71 pKa = 4.0DD72 pKa = 5.05AVNDD76 pKa = 3.92LNRR79 pKa = 11.84AEE81 pKa = 4.09AADD84 pKa = 3.79RR85 pKa = 11.84FDD87 pKa = 3.8RR88 pKa = 11.84KK89 pKa = 10.02KK90 pKa = 10.65DD91 pKa = 3.49SSMGAYY97 pKa = 9.63AGKK100 pKa = 10.04FKK102 pKa = 11.02KK103 pKa = 9.9NRR105 pKa = 11.84RR106 pKa = 11.84LKK108 pKa = 10.69KK109 pKa = 10.42SSLKK113 pKa = 10.76LKK115 pKa = 10.34ALSNGYY121 pKa = 9.11HH122 pKa = 5.76ATSEE126 pKa = 4.12HH127 pKa = 6.56FGRR130 pKa = 11.84IEE132 pKa = 4.92DD133 pKa = 4.06PNCVYY138 pKa = 10.32IHH140 pKa = 7.1HH141 pKa = 6.43STFDD145 pKa = 3.77NIQSIRR151 pKa = 11.84AISGAMLRR159 pKa = 11.84KK160 pKa = 9.59LFKK163 pKa = 10.41QAGIPLTDD171 pKa = 3.75KK172 pKa = 11.27NEE174 pKa = 4.51EE175 pKa = 3.96IPASNWEE182 pKa = 4.2SSAGTKK188 pKa = 9.28LQYY191 pKa = 10.82VHH193 pKa = 7.34LDD195 pKa = 3.49QMSGAQTEE203 pKa = 3.54IDD205 pKa = 3.73YY206 pKa = 10.93IVTNNQGLSQILANWPAMQNHH227 pKa = 6.43ILSYY231 pKa = 10.94LNGIAVTVPWKK242 pKa = 10.67LCLFIPDD249 pKa = 4.17VGPVITTHH257 pKa = 6.73RR258 pKa = 11.84CLSQISLWSEE268 pKa = 3.71HH269 pKa = 5.19LTVFQSSEE277 pKa = 3.5IKK279 pKa = 9.72VQNRR283 pKa = 11.84TKK285 pKa = 11.18GDD287 pKa = 3.56LSGVADD293 pKa = 4.39PEE295 pKa = 3.82AAAIEE300 pKa = 4.43RR301 pKa = 11.84VDD303 pKa = 3.93SQPLIARR310 pKa = 11.84TYY312 pKa = 8.03YY313 pKa = 10.64LKK315 pKa = 11.04SGDD318 pKa = 2.93IRR320 pKa = 11.84LRR322 pKa = 11.84SNAGGLANSNNVFTAAAVTGLNLIRR347 pKa = 11.84SAQLADD353 pKa = 3.54ISWQNRR359 pKa = 11.84PVPQIFSTCSKK370 pKa = 10.63SADD373 pKa = 3.83TILQPGDD380 pKa = 3.05IKK382 pKa = 10.72KK383 pKa = 8.44TFVAHH388 pKa = 6.63KK389 pKa = 10.22FSGLFLNVLIKK400 pKa = 10.01MRR402 pKa = 11.84AHH404 pKa = 6.79QINAPGTFANGCQGRR419 pKa = 11.84SEE421 pKa = 4.18LMCFEE426 pKa = 4.25EE427 pKa = 5.38KK428 pKa = 10.3IRR430 pKa = 11.84TAGTNKK436 pKa = 9.66IVIQYY441 pKa = 6.79EE442 pKa = 3.66QHH444 pKa = 5.29YY445 pKa = 9.58QAGAFLKK452 pKa = 10.48SGKK455 pKa = 9.34KK456 pKa = 9.72VPFISTLTVTEE467 pKa = 4.29KK468 pKa = 11.35NNVV471 pKa = 3.13

Molecular weight: 51.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

750

279

471

375.0

41.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.067 ± 0.665

1.733 ± 0.437

5.6 ± 0.883

4.667 ± 0.602

3.733 ± 0.118

6.933 ± 0.877

2.267 ± 0.267

6.8 ± 0.208

7.733 ± 0.287

7.467 ± 0.975

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.117

5.2 ± 0.708

3.6 ± 0.395

4.8 ± 0.079

5.333 ± 0.226

6.667 ± 0.929

6.267 ± 0.912

4.8 ± 0.685

1.6 ± 0.512

3.733 ± 0.724
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski