Microbacterium phage vB_MoxS-ISF9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Farahnazvirus; Microbacterium virus ISF9

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 120 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8NNM1|W8NNM1_9CAUD Uncharacterized protein OS=Microbacterium phage vB_MoxS-ISF9 OX=1458670 GN=ISF9_070 PE=4 SV=1
MM1 pKa = 6.57TTEE4 pKa = 4.59NDD6 pKa = 3.86HH7 pKa = 6.85LCIGHH12 pKa = 6.68DD13 pKa = 4.03LCFTMDD19 pKa = 3.26GGVPVITTRR28 pKa = 11.84EE29 pKa = 3.97YY30 pKa = 11.48DD31 pKa = 3.26NHH33 pKa = 7.04LSLGDD38 pKa = 3.92LGAIAEE44 pKa = 4.36WLGQAWEE51 pKa = 4.12VLAGEE56 pKa = 4.51KK57 pKa = 10.13YY58 pKa = 9.41KK59 pKa = 10.44PVRR62 pKa = 11.84ISEE65 pKa = 4.07RR66 pKa = 11.84GYY68 pKa = 10.2VADD71 pKa = 4.17AGEE74 pKa = 4.48IVEE77 pKa = 5.24AMYY80 pKa = 10.99DD81 pKa = 3.76PTDD84 pKa = 3.44SVYY87 pKa = 11.37DD88 pKa = 4.07DD89 pKa = 4.37GNGLEE94 pKa = 4.59RR95 pKa = 11.84DD96 pKa = 4.06CEE98 pKa = 4.09

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8NNL7|W8NNL7_9CAUD Uncharacterized protein OS=Microbacterium phage vB_MoxS-ISF9 OX=1458670 GN=ISF9_065 PE=4 SV=1
MM1 pKa = 7.33ATTHH5 pKa = 5.97TCHH8 pKa = 6.22VDD10 pKa = 2.97CRR12 pKa = 11.84LEE14 pKa = 3.95VTPYY18 pKa = 10.3TDD20 pKa = 4.05GNGKK24 pKa = 8.54PRR26 pKa = 11.84EE27 pKa = 4.04RR28 pKa = 11.84RR29 pKa = 11.84AHH31 pKa = 5.54IMYY34 pKa = 10.22AAEE37 pKa = 4.44PSSAAGRR44 pKa = 11.84YY45 pKa = 7.01QWEE48 pKa = 4.16QNRR51 pKa = 11.84AILDD55 pKa = 3.28QGRR58 pKa = 11.84RR59 pKa = 11.84AWRR62 pKa = 11.84ASGQGQSRR70 pKa = 11.84ACRR73 pKa = 11.84VWDD76 pKa = 3.58EE77 pKa = 4.06QRR79 pKa = 11.84RR80 pKa = 11.84TGANRR85 pKa = 11.84VGEE88 pKa = 4.01RR89 pKa = 11.84LFRR92 pKa = 11.84RR93 pKa = 11.84KK94 pKa = 10.22LYY96 pKa = 10.87DD97 pKa = 3.29FF98 pKa = 5.27

Molecular weight: 11.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

120

0

120

18476

24

1174

154.0

16.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.733 ± 0.358

0.622 ± 0.095

6.435 ± 0.221

7.263 ± 0.263

3.107 ± 0.155

8.519 ± 0.324

1.992 ± 0.145

4.947 ± 0.174

4.644 ± 0.241

7.042 ± 0.214

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.138

3.339 ± 0.168

4.438 ± 0.245

3.145 ± 0.158

6.479 ± 0.278

5.981 ± 0.214

6.019 ± 0.229

7.383 ± 0.197

2.111 ± 0.121

3.145 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski