Candidatus Moduliflexus flocculans

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Candidatus Moduliflexus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5960 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A081BLX2|A0A081BLX2_9BACT Uncharacterized protein OS=Candidatus Moduliflexus flocculans OX=1499966 GN=U14_02632 PE=4 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84KK3 pKa = 9.58HH4 pKa = 7.71YY5 pKa = 10.84MMFCVLLGVIHH16 pKa = 7.07LLSACGTGTKK26 pKa = 10.03RR27 pKa = 11.84SGALYY32 pKa = 9.8PPGANPGQLQCGGVEE47 pKa = 3.99PGKK50 pKa = 9.39TGTLVADD57 pKa = 3.52TGFRR61 pKa = 11.84PRR63 pKa = 11.84PNGFSFANYY72 pKa = 9.03GGGYY76 pKa = 9.46RR77 pKa = 11.84EE78 pKa = 4.66ANLNPEE84 pKa = 4.43AAWKK88 pKa = 10.44IFGEE92 pKa = 4.64TACKK96 pKa = 10.15RR97 pKa = 11.84MKK99 pKa = 10.42QEE101 pKa = 3.83EE102 pKa = 4.75CVPTPALKK110 pKa = 10.58LWVKK114 pKa = 9.24TMNEE118 pKa = 3.68AMGQGHH124 pKa = 6.73CEE126 pKa = 3.86GMATLSAWMFLAQDD140 pKa = 3.43KK141 pKa = 10.13LAAYY145 pKa = 7.61GKK147 pKa = 7.91PTAFDD152 pKa = 4.8LDD154 pKa = 3.79TTQADD159 pKa = 4.33LLQDD163 pKa = 3.04IAFYY167 pKa = 8.02WTLQTLEE174 pKa = 4.28PVVSEE179 pKa = 4.74FNAFQQQPPSEE190 pKa = 4.14ILNTLISTMQQKK202 pKa = 8.82TDD204 pKa = 2.84WYY206 pKa = 8.4TLGIYY211 pKa = 10.6GSVGGHH217 pKa = 6.12AVTPYY222 pKa = 10.62AVEE225 pKa = 4.02DD226 pKa = 3.58VGNGVFWIHH235 pKa = 6.55VYY237 pKa = 10.96DD238 pKa = 3.82NNYY241 pKa = 8.01PCAYY245 pKa = 9.44KK246 pKa = 10.65YY247 pKa = 11.33VEE249 pKa = 4.04VDD251 pKa = 3.25TNTEE255 pKa = 3.52TWHH258 pKa = 5.84YY259 pKa = 10.51AGAAINPAEE268 pKa = 4.9DD269 pKa = 3.41ASPWSGTTGTMDD281 pKa = 3.7LTSLSVRR288 pKa = 11.84QQSMEE293 pKa = 4.15CPFCSEE299 pKa = 4.81AQRR302 pKa = 11.84CLQNAGLSVLVNGRR316 pKa = 11.84GANIRR321 pKa = 11.84ATNAEE326 pKa = 4.16GKK328 pKa = 9.57QLSLQNGQAQNEE340 pKa = 4.32IPGAKK345 pKa = 9.43LLKK348 pKa = 10.41LKK350 pKa = 10.97GIFGEE355 pKa = 4.66DD356 pKa = 3.1RR357 pKa = 11.84ASLLILPPNAKK368 pKa = 9.9YY369 pKa = 10.67ALDD372 pKa = 3.99LGGIADD378 pKa = 4.16VEE380 pKa = 4.43EE381 pKa = 4.8KK382 pKa = 10.43SASIAMFTSCEE393 pKa = 4.11GYY395 pKa = 10.67ALSGMSLSSGQINQIMMTDD414 pKa = 3.42KK415 pKa = 11.24GRR417 pKa = 11.84FSYY420 pKa = 10.39QAGGAQSPTFEE431 pKa = 4.36AAFHH435 pKa = 6.44NPDD438 pKa = 3.82GNDD441 pKa = 3.05TFYY444 pKa = 11.07SISDD448 pKa = 3.38FDD450 pKa = 3.84IGDD453 pKa = 3.51GRR455 pKa = 11.84SISFEE460 pKa = 3.76QDD462 pKa = 2.19EE463 pKa = 4.83DD464 pKa = 3.47TGEE467 pKa = 4.62IYY469 pKa = 10.39IQDD472 pKa = 5.0DD473 pKa = 4.03DD474 pKa = 5.37PEE476 pKa = 4.77MEE478 pKa = 5.79DD479 pKa = 3.47FDD481 pKa = 6.69LDD483 pKa = 3.21IFTIDD488 pKa = 3.22TEE490 pKa = 4.88GEE492 pKa = 4.28VEE494 pKa = 4.41GISYY498 pKa = 10.94KK499 pKa = 11.03NIDD502 pKa = 3.81VEE504 pKa = 5.78DD505 pKa = 3.85EE506 pKa = 4.38GQVILHH512 pKa = 6.7FGDD515 pKa = 4.57DD516 pKa = 3.91EE517 pKa = 5.04SFEE520 pKa = 4.98LYY522 pKa = 9.92IDD524 pKa = 3.77SDD526 pKa = 4.08GDD528 pKa = 4.23GISDD532 pKa = 3.64SPPYY536 pKa = 9.9EE537 pKa = 4.34KK538 pKa = 10.92SNEE541 pKa = 4.07SPDD544 pKa = 3.25WDD546 pKa = 3.51NYY548 pKa = 11.15GYY550 pKa = 11.11DD551 pKa = 3.8DD552 pKa = 5.32ADD554 pKa = 3.56VSEE557 pKa = 4.69SSGNSFDD564 pKa = 5.82NNDD567 pKa = 3.93ADD569 pKa = 5.36DD570 pKa = 4.58SDD572 pKa = 5.36SEE574 pKa = 4.5TTYY577 pKa = 11.12SVNYY581 pKa = 8.82APSYY585 pKa = 8.5EE586 pKa = 4.31SEE588 pKa = 4.12WVDD591 pKa = 4.26DD592 pKa = 4.14YY593 pKa = 12.03ASEE596 pKa = 4.9AISEE600 pKa = 4.37KK601 pKa = 10.91YY602 pKa = 9.97EE603 pKa = 4.19EE604 pKa = 4.51NDD606 pKa = 3.7SDD608 pKa = 5.31HH609 pKa = 7.42SDD611 pKa = 4.82DD612 pKa = 5.86GDD614 pKa = 3.49WDD616 pKa = 4.25NNDD619 pKa = 4.81DD620 pKa = 5.94DD621 pKa = 6.22DD622 pKa = 7.28DD623 pKa = 5.79SDD625 pKa = 5.99HH626 pKa = 7.61GDD628 pKa = 3.64LDD630 pKa = 3.92NDD632 pKa = 3.67NGSDD636 pKa = 3.92YY637 pKa = 11.46DD638 pKa = 4.41DD639 pKa = 5.45GSDD642 pKa = 3.74TGSDD646 pKa = 3.75DD647 pKa = 4.57SGSDD651 pKa = 4.26DD652 pKa = 4.38SANDD656 pKa = 3.95PDD658 pKa = 4.74SSGSDD663 pKa = 3.16SSDD666 pKa = 3.31GSDD669 pKa = 3.23SGYY672 pKa = 11.54

Molecular weight: 73.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A081BQX6|A0A081BQX6_9BACT Putative ABC transporter permease protein OS=Candidatus Moduliflexus flocculans OX=1499966 GN=U14_05081 PE=4 SV=1
MM1 pKa = 7.37GRR3 pKa = 11.84LFHH6 pKa = 6.36PRR8 pKa = 11.84QRR10 pKa = 11.84EE11 pKa = 3.9LEE13 pKa = 4.18LPQRR17 pKa = 11.84QLLRR21 pKa = 11.84ARR23 pKa = 11.84RR24 pKa = 11.84SFLTMMWIIWSFDD37 pKa = 3.48PLGGLGGDD45 pKa = 3.79SPPAKK50 pKa = 10.19KK51 pKa = 10.29FFLGTTGIRR60 pKa = 11.84GRR62 pKa = 11.84KK63 pKa = 8.73DD64 pKa = 2.79RR65 pKa = 11.84GIILAYY71 pKa = 9.08TYY73 pKa = 9.38PCRR76 pKa = 11.84SFKK79 pKa = 11.01VCLTDD84 pKa = 3.67SIGRR88 pKa = 11.84GEE90 pKa = 4.54PPCSPEE96 pKa = 3.46PHH98 pKa = 6.22ARR100 pKa = 11.84CPNHH104 pKa = 6.26TRR106 pKa = 11.84DD107 pKa = 3.37ARR109 pKa = 11.84TTRR112 pKa = 11.84EE113 pKa = 3.78MPEE116 pKa = 3.57QQGRR120 pKa = 11.84HH121 pKa = 5.46GGLPLPAVVCRR132 pKa = 11.84KK133 pKa = 10.01NPLTLLHH140 pKa = 5.97TRR142 pKa = 11.84RR143 pKa = 11.84KK144 pKa = 10.29LSDD147 pKa = 3.27TFKK150 pKa = 11.08VSDD153 pKa = 3.71SSAHH157 pKa = 6.24NVLCQYY163 pKa = 11.15RR164 pKa = 11.84PMRR167 pKa = 11.84SSIAVFIASRR177 pKa = 11.84VRR179 pKa = 11.84AKK181 pKa = 10.69SSSEE185 pKa = 3.88CANEE189 pKa = 3.6TNAASNCDD197 pKa = 3.36GARR200 pKa = 3.31

Molecular weight: 22.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5960

0

5960

2000297

29

2846

335.6

37.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.121 ± 0.036

1.101 ± 0.012

5.084 ± 0.024

6.666 ± 0.033

4.556 ± 0.025

6.496 ± 0.025

2.316 ± 0.016

6.772 ± 0.026

4.693 ± 0.025

10.352 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.014

3.547 ± 0.019

4.372 ± 0.023

4.674 ± 0.024

5.701 ± 0.025

5.508 ± 0.02

5.446 ± 0.019

6.529 ± 0.025

1.218 ± 0.014

3.226 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski