Phoenicopterus ruber ruber

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; Coe

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091TU11|A0A091TU11_PHORB Mitochondrial glutamate carrier 1 (Fragment) OS=Phoenicopterus ruber ruber OX=9218 GN=N337_04182 PE=3 SV=1
DDD2 pKa = 4.19QSLPGLANVDDD13 pKa = 4.79SAQNPNIQVTIEEE26 pKa = 4.15VDDD29 pKa = 4.31DD30 pKa = 4.17QAEEE34 pKa = 4.2EEE36 pKa = 4.1DDD38 pKa = 3.99LKKK41 pKa = 10.12EE42 pKa = 4.19SNDDD46 pKa = 2.88SLTSSEEE53 pKa = 4.14LSHHH57 pKa = 7.2KK58 pKa = 10.8DD59 pKa = 3.65FWPLFWEEE67 pKa = 4.38YY68 pKa = 10.58DDD70 pKa = 3.68AEEE73 pKa = 4.28EE74 pKa = 4.14EE75 pKa = 4.18EE76 pKa = 4.36EE77 pKa = 4.18EE78 pKa = 4.26EE79 pKa = 4.41EE80 pKa = 5.32EE81 pKa = 5.2EE82 pKa = 5.12EE83 pKa = 4.63EE84 pKa = 5.57DD85 pKa = 5.08DD86 pKa = 4.82LDDD89 pKa = 3.38GDDD92 pKa = 3.97DDD94 pKa = 3.67EE95 pKa = 4.78EE96 pKa = 4.27EE97 pKa = 4.23EE98 pKa = 4.15EE99 pKa = 4.17EE100 pKa = 4.26EE101 pKa = 4.18EE102 pKa = 4.21EE103 pKa = 4.21EE104 pKa = 4.21EE105 pKa = 4.21EE106 pKa = 4.21EE107 pKa = 4.21EE108 pKa = 4.21EE109 pKa = 4.32EE110 pKa = 4.28EE111 pKa = 4.87EE112 pKa = 4.62EE113 pKa = 4.43EE114 pKa = 3.89EE115 pKa = 5.43DD116 pKa = 3.53YY117 pKa = 10.46AEEE120 pKa = 4.11YY121 pKa = 10.59EE122 pKa = 4.36EE123 pKa = 4.71EE124 pKa = 4.02EE125 pKa = 4.51MLSGVGGDDD134 pKa = 3.46DDD136 pKa = 4.48RR137 pKa = 11.84WPGQKKK143 pKa = 9.55WIFKKK148 pKa = 10.0EE149 pKa = 4.0KK150 pKa = 10.83YY151 pKa = 8.82YYY153 pKa = 10.35DD154 pKa = 3.76YY155 pKa = 11.17EE156 pKa = 5.81DD157 pKa = 3.87EE158 pKa = 4.87EE159 pKa = 4.71EE160 pKa = 4.04SPWSPCSITCGSGNQKKK177 pKa = 8.36TRR179 pKa = 11.84SCGYYY184 pKa = 9.46CTATEEE190 pKa = 5.26RR191 pKa = 11.84TCDDD195 pKa = 3.49PHHH198 pKa = 7.0PGAEEE203 pKa = 3.73EEE205 pKa = 4.48VFPTEEE211 pKa = 3.84EE212 pKa = 4.21PFKKK216 pKa = 10.91DDD218 pKa = 2.8TTEEE222 pKa = 4.22FNSEEE227 pKa = 3.77DDD229 pKa = 4.04CEEE232 pKa = 3.23KK233 pKa = 9.96LNCKKK238 pKa = 10.19DDD240 pKa = 3.71LTKKK244 pKa = 10.51YY245 pKa = 9.97SKKK248 pKa = 11.0LTDDD252 pKa = 5.3PSCPCSYYY260 pKa = 10.29LEEE263 pKa = 4.37VYYY266 pKa = 10.72AVNLQDDD273 pKa = 3.34EE274 pKa = 4.8QGK

Molecular weight: 32.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091UEQ4|A0A091UEQ4_PHORB Mitogen-activated protein kinase kinase kinase 3 (Fragment) OS=Phoenicopterus ruber ruber OX=9218 GN=N337_01822 PE=4 SV=1
TT1 pKa = 6.27QSMRR5 pKa = 11.84VTQSMKK11 pKa = 8.34VTQSMRR17 pKa = 11.84VTQSMKK23 pKa = 8.34VTQSMRR29 pKa = 11.84VTQSMKK35 pKa = 8.34VTQSMRR41 pKa = 11.84VTQSMKK47 pKa = 8.34VTQSMRR53 pKa = 11.84VTQSMKK59 pKa = 8.34VTQSMRR65 pKa = 11.84VTQSMKK71 pKa = 8.34VTQSMRR77 pKa = 11.84VTQSMKK83 pKa = 8.34VTQSMRR89 pKa = 11.84VTT91 pKa = 3.57

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8220

0

8220

3367651

31

4570

409.7

46.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.36 ± 0.023

2.242 ± 0.022

5.051 ± 0.019

7.226 ± 0.036

3.91 ± 0.02

5.774 ± 0.033

2.519 ± 0.012

5.038 ± 0.02

6.511 ± 0.036

9.698 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.011

4.199 ± 0.018

5.229 ± 0.031

4.672 ± 0.026

5.191 ± 0.022

8.257 ± 0.04

5.413 ± 0.02

6.295 ± 0.022

1.177 ± 0.01

2.99 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski