Phialocephala scopiformis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Mollisiaceae; Mollisia

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18561 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A194WX19|A0A194WX19_9HELO Uncharacterized protein (Fragment) OS=Phialocephala scopiformis OX=149040 GN=LY89DRAFT_563837 PE=4 SV=1
MM1 pKa = 7.52HH2 pKa = 7.18FFITAPLLAGLASAGFIDD20 pKa = 4.88TIEE23 pKa = 4.7SIIKK27 pKa = 9.13TSEE30 pKa = 3.75PVEE33 pKa = 4.25ALSEE37 pKa = 3.86WSYY40 pKa = 10.55TNCGTDD46 pKa = 2.84TDD48 pKa = 5.08AISLHH53 pKa = 6.42SFTLSPDD60 pKa = 3.4PPVPGSPLTATFDD73 pKa = 3.49FTANSEE79 pKa = 4.25ILEE82 pKa = 4.23GAYY85 pKa = 10.52VDD87 pKa = 3.93IDD89 pKa = 3.82VKK91 pKa = 11.11LGFIILLSKK100 pKa = 9.88TIDD103 pKa = 3.44LCDD106 pKa = 4.28DD107 pKa = 3.77SEE109 pKa = 6.27DD110 pKa = 3.58GVLYY114 pKa = 8.76DD115 pKa = 4.44TGVSCPIAAGDD126 pKa = 3.75YY127 pKa = 10.67VLTKK131 pKa = 10.83NVDD134 pKa = 3.76LPAEE138 pKa = 4.18IPPAKK143 pKa = 9.47FTVEE147 pKa = 3.82SRR149 pKa = 11.84GYY151 pKa = 9.04TFDD154 pKa = 4.17DD155 pKa = 2.97QDD157 pKa = 3.9MFCVDD162 pKa = 3.19VVANFMTTVAVRR174 pKa = 11.84EE175 pKa = 4.28GLL177 pKa = 3.58

Molecular weight: 18.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A194XIW6|A0A194XIW6_9HELO Amino acid transporter OS=Phialocephala scopiformis OX=149040 GN=LY89DRAFT_705646 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.44QKK12 pKa = 10.05LARR15 pKa = 11.84AQKK18 pKa = 8.97QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18561

0

18561

7937098

49

6547

427.6

47.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.099 ± 0.015

1.357 ± 0.008

5.457 ± 0.016

6.176 ± 0.019

3.968 ± 0.011

6.843 ± 0.016

2.305 ± 0.007

5.33 ± 0.013

5.027 ± 0.019

9.004 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.265 ± 0.008

3.856 ± 0.009

5.713 ± 0.018

3.83 ± 0.01

5.63 ± 0.017

8.382 ± 0.026

6.173 ± 0.016

6.093 ± 0.013

1.585 ± 0.007

2.905 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski