Mycolicibacterium sp. P9-64

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium; unclassified Mycolicibacterium

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6799 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5A7WRA0|A0A5A7WRA0_9MYCO MCE family protein OS=Mycolicibacterium sp. P9-64 OX=2024612 GN=CIW52_24080 PE=4 SV=1
MM1 pKa = 7.45VDD3 pKa = 3.61HH4 pKa = 6.25VQSGSADD11 pKa = 3.45KK12 pKa = 11.12VGSEE16 pKa = 4.22ASSTVSDD23 pKa = 3.59DD24 pKa = 3.37TVLRR28 pKa = 11.84RR29 pKa = 11.84SAITSAGQTASTTSGAQFSALSVPQVQTPPAPFDD63 pKa = 3.55PVGALLALPGQLFDD77 pKa = 3.6VASGFVALVITPFLAPTPNAPAQMPLLWTVLAWVRR112 pKa = 11.84RR113 pKa = 11.84EE114 pKa = 3.51ISHH117 pKa = 6.12TFFNRR122 pKa = 11.84SPIANPVQISQTVGGEE138 pKa = 3.96VTGNLNASDD147 pKa = 4.32PNGDD151 pKa = 3.58EE152 pKa = 4.07PLTYY156 pKa = 10.17TVTQQPAHH164 pKa = 5.54GTVVVRR170 pKa = 11.84PDD172 pKa = 2.95GTYY175 pKa = 10.06TYY177 pKa = 9.48TPTAGAPTSTLTDD190 pKa = 3.48SFTVTIDD197 pKa = 3.75DD198 pKa = 4.14SVGTQLPGVFGVVQGILHH216 pKa = 7.16RR217 pKa = 11.84LAQFIGIAQSDD228 pKa = 3.99TTVTQVAVTVAGAGVNLPPLVVTSVINPIPYY259 pKa = 8.89TVGSGPRR266 pKa = 11.84VLDD269 pKa = 3.22SHH271 pKa = 6.21LTVVDD276 pKa = 4.76GSSNLSGGTVSVGLGFSAGDD296 pKa = 3.66TLSYY300 pKa = 10.1TPPANNPITGSYY312 pKa = 10.39DD313 pKa = 2.99AATGVLTLSGTATVAQYY330 pKa = 11.14QDD332 pKa = 3.11ALRR335 pKa = 11.84AVSFSTTSDD344 pKa = 3.09ALIGARR350 pKa = 11.84TVSFVVTDD358 pKa = 3.9DD359 pKa = 4.46GGLSSISVPLALTVLALNLPPVVTTSVVGPIVTAGNPPVTLDD401 pKa = 3.63ANVTIIDD408 pKa = 4.11GSGSMTGAQVSLGLGFSAGDD428 pKa = 3.55TLGYY432 pKa = 7.62TAPQSNPITGSYY444 pKa = 9.68NAATGVLTLSGTGTVAQYY462 pKa = 10.84QEE464 pKa = 4.14ALRR467 pKa = 11.84AVTFSTTSNALVGVRR482 pKa = 11.84TVGFVVTDD490 pKa = 3.79DD491 pKa = 4.67GGLSSISVPLAVTVLALNLPPVVTTSVIGPIVTAGNPPVTLDD533 pKa = 3.57GNVTIVDD540 pKa = 4.48GSASMTGGTVSLGLGFSPGDD560 pKa = 3.52TLGYY564 pKa = 7.54TAPQNNPITGSYY576 pKa = 9.61NAATGVLTLSGTGTVAQYY594 pKa = 10.84QEE596 pKa = 4.14ALRR599 pKa = 11.84AVTFSTTSNALVGVRR614 pKa = 11.84TVGFVVTDD622 pKa = 3.79DD623 pKa = 4.67GGLSSISVPLAVTVLALNLPPVVTTSVIGPIVTAGNPPVTLDD665 pKa = 3.53ANVTIVDD672 pKa = 4.42GSASLTGGTVSLGLGFSAGDD692 pKa = 3.55TLGYY696 pKa = 7.61TAPQDD701 pKa = 3.69NPITGSYY708 pKa = 9.64NAATGVLTLSGTGTVAQYY726 pKa = 10.84QEE728 pKa = 4.14ALRR731 pKa = 11.84AVTFSTTSNALVGVRR746 pKa = 11.84TVGFVVTDD754 pKa = 3.79DD755 pKa = 4.67GGLSSISVPLAVTVLALNLPPVVTTSVVGPIVTAGNPPVTLDD797 pKa = 3.53ANVTIVDD804 pKa = 4.59GSASMTGGTVSLGLGFGPGDD824 pKa = 3.56TLGYY828 pKa = 7.54TAPQNNPITGSYY840 pKa = 9.61NAATGVLTLSGTGTVAQYY858 pKa = 10.84QEE860 pKa = 4.14ALRR863 pKa = 11.84AVTFSTTAGALVGVRR878 pKa = 11.84TVGFVVTDD886 pKa = 3.19VGGLSSISVPLAVTVLALNLPPVVTTSVIGPIVTAGNPPVTLDD929 pKa = 3.53ANVTIVDD936 pKa = 4.42GSASLTGGTVSVGLGFSAGDD956 pKa = 3.55TLGYY960 pKa = 7.6TAPQNNPITGSYY972 pKa = 10.39DD973 pKa = 2.99AATGVLTLSGTGTVAQYY990 pKa = 10.88QEE992 pKa = 4.36ALRR995 pKa = 11.84SITFSTTAGVLVGARR1010 pKa = 11.84TVSFVVTDD1018 pKa = 3.4VEE1020 pKa = 4.79GASSISVPLAVTVLALNLPPVVTTSVVGPIVTAGNPPVTLDD1061 pKa = 3.53ANVTIVDD1068 pKa = 4.42GSASLTGGTVSLGLGFSAGDD1088 pKa = 3.55TLGYY1092 pKa = 7.61TAPQDD1097 pKa = 3.69NPITGSYY1104 pKa = 10.4DD1105 pKa = 2.99AATGVLTLSGTGTVAQYY1122 pKa = 10.88QEE1124 pKa = 4.36ALRR1127 pKa = 11.84SITFSTTAGALVGARR1142 pKa = 11.84TVSFVVTDD1150 pKa = 3.4VEE1152 pKa = 4.79GASSISVPLAVTVLALNLPPVVTTSVVGPLVTAGNPPVTLDD1193 pKa = 3.53ANVTIVDD1200 pKa = 4.42GSASLTGGTVSLGLGFSAGDD1220 pKa = 3.55TLGYY1224 pKa = 7.61TAPQDD1229 pKa = 3.69NPITGSYY1236 pKa = 10.4DD1237 pKa = 2.99AATGVLTLSGTGTVAQYY1254 pKa = 10.88QEE1256 pKa = 4.36ALRR1259 pKa = 11.84SITFSTTAGALVGARR1274 pKa = 11.84TVSFVVTDD1282 pKa = 3.4VEE1284 pKa = 4.79GASSISVPLAVTVLALNLPPVVTTSVIGPIVTAGSPPVTLDD1325 pKa = 3.33ANVTIVDD1332 pKa = 4.42GSASLTGGTVSLGLGFSAGDD1352 pKa = 3.55TLGYY1356 pKa = 7.6TAPQNNPITGSYY1368 pKa = 10.71NSATGVLTLSGTGTVAQYY1386 pKa = 10.88QEE1388 pKa = 4.36ALRR1391 pKa = 11.84SITFSTTAGALVGARR1406 pKa = 11.84TVSFVVTDD1414 pKa = 3.4VEE1416 pKa = 4.79GASSISVPLAVTVLALNLPPVVTTSVIGPIVTAGNSPVTLDD1457 pKa = 3.57ANVTIVDD1464 pKa = 4.42GSASLTGGTVSVGLGFSAGDD1484 pKa = 3.55TLGYY1488 pKa = 7.61TAPQDD1493 pKa = 3.69NPITGSYY1500 pKa = 10.4DD1501 pKa = 2.99AATGVLTLSGTGTVAQYY1518 pKa = 10.88QEE1520 pKa = 4.36ALRR1523 pKa = 11.84SITFSTTAGALVGARR1538 pKa = 11.84TVSFVVTDD1546 pKa = 3.4VEE1548 pKa = 4.79GASSISVPLAVTVLALNVPPVVVTSILNLIPFTAGNAPATLDD1590 pKa = 3.69PNLTVIDD1597 pKa = 4.86GSASLSGATVAITVGLTAGDD1617 pKa = 3.93SLAFTQPPGSSITGSYY1633 pKa = 10.85NSATGVLTLAGTGTVAQYY1651 pKa = 10.85QEE1653 pKa = 4.29ALRR1656 pKa = 11.84SVTFATTTATLVGVRR1671 pKa = 11.84TVSFVVTDD1679 pKa = 3.58VEE1681 pKa = 4.5GLPSVSVPLAVTVALNLPPVVTSSVVGLSLNLLNDD1716 pKa = 4.63APPKK1720 pKa = 10.43ILDD1723 pKa = 3.96PGVLIVDD1730 pKa = 4.61DD1731 pKa = 4.91SSTLTGATVTVGGLVIGGNDD1751 pKa = 3.18TLAFTSPPGSGITGVWNAGTKK1772 pKa = 9.2TLTLSGAATVADD1784 pKa = 4.14YY1785 pKa = 8.47QTALRR1790 pKa = 11.84SVTFQTSGFLNLGARR1805 pKa = 11.84VVSFVVKK1812 pKa = 10.19DD1813 pKa = 3.54QQGQSSISVPLTVVVVGILL1832 pKa = 3.21

Molecular weight: 180.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5A7WKF1|A0A5A7WKF1_9MYCO TetR/AcrR family transcriptional regulator OS=Mycolicibacterium sp. P9-64 OX=2024612 GN=CIW52_20695 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSGRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.81GRR42 pKa = 11.84RR43 pKa = 11.84SLTAA47 pKa = 3.9

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6799

0

6799

2191175

27

7611

322.3

34.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.891 ± 0.035

0.792 ± 0.008

6.388 ± 0.024

5.123 ± 0.026

3.1 ± 0.017

8.943 ± 0.033

2.206 ± 0.014

4.382 ± 0.019

2.255 ± 0.018

9.777 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.012

2.295 ± 0.015

5.688 ± 0.026

2.913 ± 0.016

6.883 ± 0.028

5.596 ± 0.019

6.216 ± 0.029

8.876 ± 0.028

1.477 ± 0.013

2.141 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski