Burkholderia phage KS9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4FAS6|Q4FAS6_9CAUD Minor tail protein gp12 OS=Burkholderia phage KS9 OX=335797 PE=4 SV=2
MM1 pKa = 7.14SALVVRR7 pKa = 11.84NAIGSVGAAKK17 pKa = 10.43GYY19 pKa = 10.84AGAAATGAKK28 pKa = 9.5SPYY31 pKa = 8.29YY32 pKa = 9.78VVSRR36 pKa = 11.84VSGARR41 pKa = 11.84DD42 pKa = 3.34MAIGGATGGKK52 pKa = 9.51SGVYY56 pKa = 10.14QIDD59 pKa = 4.33AYY61 pKa = 10.98ASTYY65 pKa = 10.27TDD67 pKa = 3.46ADD69 pKa = 3.54ALADD73 pKa = 3.93RR74 pKa = 11.84VIEE77 pKa = 3.9RR78 pKa = 11.84AYY80 pKa = 10.34AAEE83 pKa = 4.02QFSVGGVNDD92 pKa = 4.3LPDD95 pKa = 5.32DD96 pKa = 4.2YY97 pKa = 11.71SSDD100 pKa = 3.42SGDD103 pKa = 3.18FRR105 pKa = 11.84VSLEE109 pKa = 3.71ISVEE113 pKa = 4.12FF114 pKa = 4.48

Molecular weight: 11.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7BGG6|C7BGG6_9CAUD Uncharacterized protein OS=Burkholderia phage KS9 OX=335797 PE=4 SV=1
MM1 pKa = 7.4PRR3 pKa = 11.84KK4 pKa = 10.01APTQCRR10 pKa = 11.84HH11 pKa = 5.49YY12 pKa = 10.82GCGRR16 pKa = 11.84LVATPGYY23 pKa = 8.76CAEE26 pKa = 4.47HH27 pKa = 7.01ASEE30 pKa = 4.14AVGWQSDD37 pKa = 3.6RR38 pKa = 11.84QRR40 pKa = 11.84GSRR43 pKa = 11.84HH44 pKa = 4.27ARR46 pKa = 11.84GYY48 pKa = 10.56GSDD51 pKa = 2.95WTKK54 pKa = 10.87LRR56 pKa = 11.84RR57 pKa = 11.84EE58 pKa = 3.84ALARR62 pKa = 11.84DD63 pKa = 3.45NGLCVPCRR71 pKa = 11.84KK72 pKa = 9.63KK73 pKa = 11.15GRR75 pKa = 11.84IARR78 pKa = 11.84AIAVDD83 pKa = 4.32HH84 pKa = 6.47IVSKK88 pKa = 11.56AEE90 pKa = 4.0GGTDD94 pKa = 3.26EE95 pKa = 4.58LTNLQSICKK104 pKa = 8.39PCHH107 pKa = 5.75DD108 pKa = 4.8AKK110 pKa = 10.44TSAEE114 pKa = 4.01AARR117 pKa = 11.84GRR119 pKa = 11.84SRR121 pKa = 11.84RR122 pKa = 3.66

Molecular weight: 13.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12580

21

1366

251.6

27.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.591 ± 0.861

0.922 ± 0.181

6.28 ± 0.234

5.795 ± 0.322

3.005 ± 0.207

8.196 ± 0.365

1.797 ± 0.215

4.944 ± 0.241

3.863 ± 0.242

7.886 ± 0.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.353 ± 0.177

3.18 ± 0.207

4.595 ± 0.403

3.609 ± 0.283

7.599 ± 0.385

6.518 ± 0.314

5.159 ± 0.21

7.385 ± 0.323

1.677 ± 0.173

2.647 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski