Planomonospora sphaerica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae;

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A171DKX2|A0A171DKX2_9ACTN Flagellar basal body rod protein FlgB OS=Planomonospora sphaerica OX=161355 GN=PS9374_05152 PE=3 SV=1
MM1 pKa = 7.7PAGSPLRR8 pKa = 11.84LPFLRR13 pKa = 11.84SGPARR18 pKa = 11.84RR19 pKa = 11.84AAAADD24 pKa = 3.66APAEE28 pKa = 4.16SLALATTTLAYY39 pKa = 9.99VVNGGDD45 pKa = 3.62GTVSVIDD52 pKa = 3.66TTTNTVTATITVGNDD67 pKa = 3.24PYY69 pKa = 10.75RR70 pKa = 11.84VAVTPDD76 pKa = 2.87GTTAYY81 pKa = 10.33VPNADD86 pKa = 3.76GGTVSVIDD94 pKa = 3.69TTTNTVTATITVGNSPYY111 pKa = 10.57GVAVTPDD118 pKa = 3.14GTTAYY123 pKa = 10.3VPNSDD128 pKa = 3.67GGTVSVIDD136 pKa = 3.69TTTNTVTATITVGNDD151 pKa = 3.38PLGVAVSPDD160 pKa = 3.05GATAYY165 pKa = 10.11VVNQSDD171 pKa = 3.91DD172 pKa = 3.77TVSVIDD178 pKa = 3.83TATGTVTATIDD189 pKa = 3.26VDD191 pKa = 3.67DD192 pKa = 5.47RR193 pKa = 11.84PISVAFGPDD202 pKa = 2.79GATAYY207 pKa = 9.88VANPGDD213 pKa = 3.85GTVSVIDD220 pKa = 3.81TATGTVTATITVGNSPYY237 pKa = 10.83AVAVTPDD244 pKa = 3.11GATAYY249 pKa = 10.73VPGNSGGTVSVIDD262 pKa = 3.81TATGTVVDD270 pKa = 5.4TITVSADD277 pKa = 3.25PYY279 pKa = 10.58DD280 pKa = 4.02AAVSPDD286 pKa = 3.1GATVYY291 pKa = 8.78VTHH294 pKa = 7.28EE295 pKa = 4.65DD296 pKa = 3.46GGTVSVIDD304 pKa = 3.81TATGTVVDD312 pKa = 5.47TITAGDD318 pKa = 4.04DD319 pKa = 3.44PRR321 pKa = 11.84GVAVAAVPVSSAVTLTASPDD341 pKa = 3.45PAQVHH346 pKa = 5.81RR347 pKa = 11.84PVTLTAQVTCTTGTPAGTVRR367 pKa = 11.84FYY369 pKa = 11.46DD370 pKa = 4.11GAALLGTRR378 pKa = 11.84PLNAAGTAVLTTSGLDD394 pKa = 3.42AGDD397 pKa = 3.98HH398 pKa = 6.17QITAVYY404 pKa = 9.63SGDD407 pKa = 4.24GICPPAVSPPLLLTVNKK424 pKa = 9.78GVSSVQVLAVPNPALVFLPVTLTARR449 pKa = 11.84VTCDD453 pKa = 2.69SDD455 pKa = 3.74IPTGTVRR462 pKa = 11.84FMDD465 pKa = 4.13GAVTLGTVPVLAGAAVLITAFRR487 pKa = 11.84SRR489 pKa = 11.84GPHH492 pKa = 6.06QITAIYY498 pKa = 10.1SGDD501 pKa = 3.74ADD503 pKa = 4.54CEE505 pKa = 4.26PSASPPRR512 pKa = 11.84TVTVIGLLL520 pKa = 3.64

Molecular weight: 51.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A161LL11|A0A161LL11_9ACTN ATPase OS=Planomonospora sphaerica OX=161355 GN=PS9374_05774 PE=4 SV=1
MM1 pKa = 6.82WRR3 pKa = 11.84ARR5 pKa = 11.84SSWPSSRR12 pKa = 11.84RR13 pKa = 11.84WASSFVRR20 pKa = 11.84RR21 pKa = 11.84PRR23 pKa = 11.84PSKK26 pKa = 10.48HH27 pKa = 5.31RR28 pKa = 11.84WSAGSRR34 pKa = 11.84KK35 pKa = 9.24LWACPRR41 pKa = 11.84RR42 pKa = 11.84VRR44 pKa = 11.84PTRR47 pKa = 11.84VPEE50 pKa = 4.02GRR52 pKa = 11.84PGRR55 pKa = 11.84LRR57 pKa = 11.84RR58 pKa = 11.84PPGRR62 pKa = 11.84PRR64 pKa = 11.84ARR66 pKa = 11.84PAALRR71 pKa = 11.84RR72 pKa = 11.84PPRR75 pKa = 11.84FPGRR79 pKa = 11.84DD80 pKa = 3.14RR81 pKa = 11.84LPSRR85 pKa = 11.84VRR87 pKa = 11.84GRR89 pKa = 11.84LRR91 pKa = 11.84VLRR94 pKa = 11.84PPRR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84PRR101 pKa = 11.84SPRR104 pKa = 11.84FSSSPRR110 pKa = 11.84SSSSRR115 pKa = 11.84RR116 pKa = 11.84CSSSRR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84SSRR126 pKa = 11.84RR127 pKa = 11.84VSRR130 pKa = 11.84RR131 pKa = 11.84LRR133 pKa = 11.84PRR135 pKa = 11.84GPRR138 pKa = 11.84APAVPVPSRR147 pKa = 11.84ARR149 pKa = 11.84ARR151 pKa = 11.84ARR153 pKa = 11.84ARR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84VPRR160 pKa = 11.84RR161 pKa = 11.84PARR164 pKa = 11.84RR165 pKa = 11.84PVPPAVPVPLRR176 pKa = 11.84ARR178 pKa = 11.84VPPPGLPVVPVPSRR192 pKa = 11.84VRR194 pKa = 11.84VRR196 pKa = 11.84ARR198 pKa = 11.84AARR201 pKa = 11.84DD202 pKa = 3.07RR203 pKa = 11.84ATTRR207 pKa = 11.84SPRR210 pKa = 11.84TPAAWARR217 pKa = 11.84PVRR220 pKa = 11.84RR221 pKa = 11.84VRR223 pKa = 11.84AAPAVPVTAVPVPAAVPVRR242 pKa = 11.84TAARR246 pKa = 11.84ATVRR250 pKa = 11.84LRR252 pKa = 11.84VPRR255 pKa = 11.84APVPARR261 pKa = 11.84PVRR264 pKa = 11.84PVPVRR269 pKa = 11.84GPVRR273 pKa = 11.84AVRR276 pKa = 11.84VRR278 pKa = 11.84AAVPARR284 pKa = 11.84PVRR287 pKa = 11.84APAAARR293 pKa = 11.84VPAVPVPEE301 pKa = 4.06VRR303 pKa = 11.84VPARR307 pKa = 3.43

Molecular weight: 34.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7159

0

7159

2355000

29

11486

329.0

35.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.06 ± 0.035

0.775 ± 0.008

5.666 ± 0.024

5.834 ± 0.028

2.716 ± 0.016

9.867 ± 0.034

2.162 ± 0.015

3.196 ± 0.019

1.748 ± 0.02

10.497 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.797 ± 0.013

1.551 ± 0.015

6.337 ± 0.031

2.405 ± 0.02

8.697 ± 0.033

4.875 ± 0.019

5.655 ± 0.024

8.708 ± 0.031

1.502 ± 0.012

1.95 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski