Amino acid dipepetide frequency for Nicotiana sylvestris (Wood tobacco) (South American tobacco)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.918AlaAla: 5.918 ± 0.029	1.184AlaCys: 1.184 ± 0.009	3.107AlaAsp: 3.107 ± 0.015	4.267AlaGlu: 4.267 ± 0.019	2.674AlaPhe: 2.674 ± 0.014	3.806AlaGly: 3.806 ± 0.018	1.286AlaHis: 1.286 ± 0.009	3.783AlaIle: 3.783 ± 0.016	4.115AlaLys: 4.115 ± 0.019	6.285AlaLeu: 6.285 ± 0.023	1.669AlaMet: 1.669 ± 0.011	2.692AlaAsn: 2.692 ± 0.014	2.724AlaPro: 2.724 ± 0.018	2.146AlaGln: 2.146 ± 0.012	3.296AlaArg: 3.296 ± 0.015	5.748AlaSer: 5.748 ± 0.023	3.577AlaThr: 3.577 ± 0.018	4.524AlaVal: 4.524 ± 0.018	0.745AlaTrp: 0.745 ± 0.007	1.837AlaTyr: 1.837 ± 0.011	0.002AlaXaa: 0.002 ± 0.0
Cys	0.968CysAla: 0.968 ± 0.007	0.545CysCys: 0.545 ± 0.007	0.924CysAsp: 0.924 ± 0.007	0.915CysGlu: 0.915 ± 0.007	0.883CysPhe: 0.883 ± 0.008	1.358CysGly: 1.358 ± 0.011	0.479CysHis: 0.479 ± 0.005	1.073CysIle: 1.073 ± 0.009	1.182CysLys: 1.182 ± 0.009	1.85CysLeu: 1.85 ± 0.011	0.453CysMet: 0.453 ± 0.005	0.871CysAsn: 0.871 ± 0.007	0.947CysPro: 0.947 ± 0.009	0.648CysGln: 0.648 ± 0.007	1.053CysArg: 1.053 ± 0.008	1.845CysSer: 1.845 ± 0.011	0.905CysThr: 0.905 ± 0.007	1.021CysVal: 1.021 ± 0.008	0.248CysTrp: 0.248 ± 0.004	0.584CysTyr: 0.584 ± 0.006	0.001CysXaa: 0.001 ± 0.0
Asp	3.376AspAla: 3.376 ± 0.015	0.974AspCys: 0.974 ± 0.008	3.566AspAsp: 3.566 ± 0.02	4.132AspGlu: 4.132 ± 0.019	2.424AspPhe: 2.424 ± 0.013	3.472AspGly: 3.472 ± 0.019	1.234AspHis: 1.234 ± 0.009	3.247AspIle: 3.247 ± 0.015	2.908AspLys: 2.908 ± 0.018	5.017AspLeu: 5.017 ± 0.02	1.379AspMet: 1.379 ± 0.009	2.217AspAsn: 2.217 ± 0.014	2.475AspPro: 2.475 ± 0.014	1.803AspGln: 1.803 ± 0.011	2.338AspArg: 2.338 ± 0.016	4.22AspSer: 4.22 ± 0.02	2.249AspThr: 2.249 ± 0.012	3.633AspVal: 3.633 ± 0.018	0.68AspTrp: 0.68 ± 0.006	1.584AspTyr: 1.584 ± 0.01	0.001AspXaa: 0.001 ± 0.0
Glu	4.669GluAla: 4.669 ± 0.021	0.952GluCys: 0.952 ± 0.008	4.005GluAsp: 4.005 ± 0.02	6.392GluGlu: 6.392 ± 0.039	2.425GluPhe: 2.425 ± 0.013	3.589GluGly: 3.589 ± 0.016	1.323GluHis: 1.323 ± 0.01	3.98GluIle: 3.98 ± 0.016	5.125GluLys: 5.125 ± 0.027	6.161GluLeu: 6.161 ± 0.025	1.841GluMet: 1.841 ± 0.011	3.348GluAsn: 3.348 ± 0.017	2.084GluPro: 2.084 ± 0.015	2.31GluGln: 2.31 ± 0.013	3.391GluArg: 3.391 ± 0.019	4.71GluSer: 4.71 ± 0.018	3.08GluThr: 3.08 ± 0.015	4.404GluVal: 4.404 ± 0.018	0.729GluTrp: 0.729 ± 0.007	1.784GluTyr: 1.784 ± 0.011	0.002GluXaa: 0.002 ± 0.0
Phe	2.439PheAla: 2.439 ± 0.013	0.905PheCys: 0.905 ± 0.007	2.379PheAsp: 2.379 ± 0.014	2.369PheGlu: 2.369 ± 0.013	1.897PhePhe: 1.897 ± 0.013	2.945PheGly: 2.945 ± 0.016	1.114PheHis: 1.114 ± 0.009	2.154PheIle: 2.154 ± 0.013	2.218PheLys: 2.218 ± 0.011	4.322PheLeu: 4.322 ± 0.019	0.962PheMet: 0.962 ± 0.008	1.845PheAsn: 1.845 ± 0.011	2.137PhePro: 2.137 ± 0.012	1.656PheGln: 1.656 ± 0.012	2.001PheArg: 2.001 ± 0.011	4.056PheSer: 4.056 ± 0.017	2.013PheThr: 2.013 ± 0.012	2.694PheVal: 2.694 ± 0.013	0.554PheTrp: 0.554 ± 0.007	1.263PheTyr: 1.263 ± 0.009	0.002PheXaa: 0.002 ± 0.0
Gly	3.627GlyAla: 3.627 ± 0.018	1.199GlyCys: 1.199 ± 0.009	3.228GlyAsp: 3.228 ± 0.015	3.607GlyGlu: 3.607 ± 0.015	2.897GlyPhe: 2.897 ± 0.012	4.9GlyGly: 4.9 ± 0.038	1.532GlyHis: 1.532 ± 0.011	3.513GlyIle: 3.513 ± 0.016	4.117GlyLys: 4.117 ± 0.015	5.472GlyLeu: 5.472 ± 0.02	1.445GlyMet: 1.445 ± 0.01	3.109GlyAsn: 3.109 ± 0.016	2.309GlyPro: 2.309 ± 0.013	2.12GlyGln: 2.12 ± 0.013	3.478GlyArg: 3.478 ± 0.014	5.674GlySer: 5.674 ± 0.023	3.139GlyThr: 3.139 ± 0.015	3.929GlyVal: 3.929 ± 0.018	0.831GlyTrp: 0.831 ± 0.007	2.039GlyTyr: 2.039 ± 0.013	0.002GlyXaa: 0.002 ± 0.0
His	1.378HisAla: 1.378 ± 0.01	0.524HisCys: 0.524 ± 0.006	1.14HisAsp: 1.14 ± 0.009	1.317HisGlu: 1.317 ± 0.011	1.126HisPhe: 1.126 ± 0.008	1.57HisGly: 1.57 ± 0.011	0.843HisHis: 0.843 ± 0.009	1.327HisIle: 1.327 ± 0.009	1.254HisLys: 1.254 ± 0.009	2.486HisLeu: 2.486 ± 0.012	0.58HisMet: 0.58 ± 0.006	1.027HisAsn: 1.027 ± 0.008	1.308HisPro: 1.308 ± 0.009	1.037HisGln: 1.037 ± 0.008	1.274HisArg: 1.274 ± 0.011	1.956HisSer: 1.956 ± 0.012	0.991HisThr: 0.991 ± 0.008	1.532HisVal: 1.532 ± 0.01	0.296HisTrp: 0.296 ± 0.004	0.718HisTyr: 0.718 ± 0.007	0.001HisXaa: 0.001 ± 0.0
Ile	3.547IleAla: 3.547 ± 0.017	1.177IleCys: 1.177 ± 0.009	2.987IleAsp: 2.987 ± 0.013	3.388IleGlu: 3.388 ± 0.015	2.423IlePhe: 2.423 ± 0.014	3.437IleGly: 3.437 ± 0.017	1.335IleHis: 1.335 ± 0.009	3.018IleIle: 3.018 ± 0.015	3.181IleLys: 3.181 ± 0.014	5.463IleLeu: 5.463 ± 0.02	1.213IleMet: 1.213 ± 0.008	2.339IleAsn: 2.339 ± 0.012	3.059IlePro: 3.059 ± 0.019	2.135IleGln: 2.135 ± 0.011	2.697IleArg: 2.697 ± 0.012	5.105IleSer: 5.105 ± 0.019	2.672IleThr: 2.672 ± 0.013	3.523IleVal: 3.523 ± 0.015	0.773IleTrp: 0.773 ± 0.007	1.571IleTyr: 1.571 ± 0.01	0.002IleXaa: 0.002 ± 0.0
Lys	4.145LysAla: 4.145 ± 0.02	1.08LysCys: 1.08 ± 0.009	3.459LysAsp: 3.459 ± 0.016	5.071LysGlu: 5.071 ± 0.028	2.444LysPhe: 2.444 ± 0.01	3.669LysGly: 3.669 ± 0.016	1.371LysHis: 1.371 ± 0.009	3.496LysIle: 3.496 ± 0.014	5.218LysLys: 5.218 ± 0.026	6.387LysLeu: 6.387 ± 0.025	1.63LysMet: 1.63 ± 0.011	2.911LysAsn: 2.911 ± 0.015	2.664LysPro: 2.664 ± 0.016	2.463LysGln: 2.463 ± 0.015	3.751LysArg: 3.751 ± 0.019	4.904LysSer: 4.904 ± 0.022	2.966LysThr: 2.966 ± 0.014	4.045LysVal: 4.045 ± 0.016	0.848LysTrp: 0.848 ± 0.008	1.837LysTyr: 1.837 ± 0.012	0.002LysXaa: 0.002 ± 0.0
Leu	6.327LeuAla: 6.327 ± 0.021	1.835LeuCys: 1.835 ± 0.013	5.039LeuAsp: 5.039 ± 0.018	6.458LeuGlu: 6.458 ± 0.028	3.756LeuPhe: 3.756 ± 0.018	5.523LeuGly: 5.523 ± 0.021	2.543LeuHis: 2.543 ± 0.013	4.725LeuIle: 4.725 ± 0.019	6.495LeuLys: 6.495 ± 0.024	9.753LeuLeu: 9.753 ± 0.034	2.203LeuMet: 2.203 ± 0.012	4.082LeuAsn: 4.082 ± 0.016	5.091LeuPro: 5.091 ± 0.024	4.412LeuGln: 4.412 ± 0.019	5.291LeuArg: 5.291 ± 0.018	8.355LeuSer: 8.355 ± 0.031	4.35LeuThr: 4.35 ± 0.018	6.233LeuVal: 6.233 ± 0.024	1.189LeuTrp: 1.189 ± 0.009	2.581LeuTyr: 2.581 ± 0.017	0.004LeuXaa: 0.004 ± 0.001
Met	2.041MetAla: 2.041 ± 0.012	0.351MetCys: 0.351 ± 0.005	1.471MetAsp: 1.471 ± 0.01	1.973MetGlu: 1.973 ± 0.01	0.807MetPhe: 0.807 ± 0.007	1.504MetGly: 1.504 ± 0.011	0.556MetHis: 0.556 ± 0.006	1.215MetIle: 1.215 ± 0.009	1.712MetLys: 1.712 ± 0.009	2.221MetLeu: 2.221 ± 0.013	0.665MetMet: 0.665 ± 0.007	1.096MetAsn: 1.096 ± 0.01	1.096MetPro: 1.096 ± 0.009	1.019MetGln: 1.019 ± 0.01	1.232MetArg: 1.232 ± 0.008	1.801MetSer: 1.801 ± 0.01	1.113MetThr: 1.113 ± 0.009	1.615MetVal: 1.615 ± 0.011	0.279MetTrp: 0.279 ± 0.004	0.593MetTyr: 0.593 ± 0.006	0.001MetXaa: 0.001 ± 0.0
Asn	2.708AsnAla: 2.708 ± 0.013	0.911AsnCys: 0.911 ± 0.008	2.263AsnAsp: 2.263 ± 0.013	2.807AsnGlu: 2.807 ± 0.015	2.095AsnPhe: 2.095 ± 0.012	3.273AsnGly: 3.273 ± 0.015	1.108AsnHis: 1.108 ± 0.009	2.745AsnIle: 2.745 ± 0.013	2.678AsnLys: 2.678 ± 0.015	4.666AsnLeu: 4.666 ± 0.022	1.196AsnMet: 1.196 ± 0.008	2.585AsnAsn: 2.585 ± 0.023	2.275AsnPro: 2.275 ± 0.012	1.837AsnGln: 1.837 ± 0.012	2.069AsnArg: 2.069 ± 0.012	4.145AsnSer: 4.145 ± 0.022	2.041AsnThr: 2.041 ± 0.011	2.892AsnVal: 2.892 ± 0.014	0.59AsnTrp: 0.59 ± 0.005	1.434AsnTyr: 1.434 ± 0.011	0.002AsnXaa: 0.002 ± 0.0
Pro	2.9ProAla: 2.9 ± 0.016	0.781ProCys: 0.781 ± 0.006	2.331ProAsp: 2.331 ± 0.014	3.035ProGlu: 3.035 ± 0.016	2.001ProPhe: 2.001 ± 0.012	2.552ProGly: 2.552 ± 0.014	1.083ProHis: 1.083 ± 0.008	2.436ProIle: 2.436 ± 0.011	2.852ProLys: 2.852 ± 0.014	4.223ProLeu: 4.223 ± 0.018	0.946ProMet: 0.946 ± 0.008	2.32ProAsn: 2.32 ± 0.014	3.682ProPro: 3.682 ± 0.046	1.859ProGln: 1.859 ± 0.012	2.291ProArg: 2.291 ± 0.015	4.955ProSer: 4.955 ± 0.023	2.654ProThr: 2.654 ± 0.013	2.981ProVal: 2.981 ± 0.016	0.578ProTrp: 0.578 ± 0.006	1.369ProTyr: 1.369 ± 0.011	0.002ProXaa: 0.002 ± 0.0
Gln	2.415GlnAla: 2.415 ± 0.014	0.614GlnCys: 0.614 ± 0.006	1.722GlnAsp: 1.722 ± 0.01	2.563GlnGlu: 2.563 ± 0.014	1.463GlnPhe: 1.463 ± 0.009	2.139GlnGly: 2.139 ± 0.012	0.99GlnHis: 0.99 ± 0.009	2.086GlnIle: 2.086 ± 0.011	2.577GlnLys: 2.577 ± 0.015	3.888GlnLeu: 3.888 ± 0.017	1.055GlnMet: 1.055 ± 0.009	1.957GlnAsn: 1.957 ± 0.012	1.744GlnPro: 1.744 ± 0.011	2.28GlnGln: 2.28 ± 0.027	2.181GlnArg: 2.181 ± 0.012	2.987GlnSer: 2.987 ± 0.016	1.746GlnThr: 1.746 ± 0.011	2.439GlnVal: 2.439 ± 0.013	0.475GlnTrp: 0.475 ± 0.005	1.002GlnTyr: 1.002 ± 0.009	0.002GlnXaa: 0.002 ± 0.0
Arg	3.163ArgAla: 3.163 ± 0.015	0.968ArgCys: 0.968 ± 0.008	2.619ArgAsp: 2.619 ± 0.015	3.344ArgGlu: 3.344 ± 0.017	2.151ArgPhe: 2.151 ± 0.012	3.173ArgGly: 3.173 ± 0.015	1.279ArgHis: 1.279 ± 0.009	2.88ArgIle: 2.88 ± 0.014	3.916ArgLys: 3.916 ± 0.019	4.892ArgLeu: 4.892 ± 0.021	1.299ArgMet: 1.299 ± 0.009	2.527ArgAsn: 2.527 ± 0.013	2.308ArgPro: 2.308 ± 0.014	1.889ArgGln: 1.889 ± 0.012	3.836ArgArg: 3.836 ± 0.022	4.299ArgSer: 4.299 ± 0.021	2.447ArgThr: 2.447 ± 0.012	3.213ArgVal: 3.213 ± 0.016	0.741ArgTrp: 0.741 ± 0.007	1.509ArgTyr: 1.509 ± 0.01	0.002ArgXaa: 0.002 ± 0.0
Ser	5.323SerAla: 5.323 ± 0.02	1.776SerCys: 1.776 ± 0.011	4.391SerAsp: 4.391 ± 0.018	4.84SerGlu: 4.84 ± 0.018	3.916SerPhe: 3.916 ± 0.02	5.605SerGly: 5.605 ± 0.026	1.985SerHis: 1.985 ± 0.012	4.72SerIle: 4.72 ± 0.02	5.142SerLys: 5.142 ± 0.018	8.362SerLeu: 8.362 ± 0.028	2.068SerMet: 2.068 ± 0.013	4.19SerAsn: 4.19 ± 0.018	4.446SerPro: 4.446 ± 0.028	3.165SerGln: 3.165 ± 0.016	4.523SerArg: 4.523 ± 0.023	10.876SerSer: 10.876 ± 0.042	4.845SerThr: 4.845 ± 0.017	5.143SerVal: 5.143 ± 0.016	1.154SerTrp: 1.154 ± 0.009	2.427SerTyr: 2.427 ± 0.012	0.003SerXaa: 0.003 ± 0.0
Thr	3.267ThrAla: 3.267 ± 0.015	0.965ThrCys: 0.965 ± 0.007	2.299ThrAsp: 2.299 ± 0.012	2.85ThrGlu: 2.85 ± 0.014	2.076ThrPhe: 2.076 ± 0.012	3.075ThrGly: 3.075 ± 0.015	1.039ThrHis: 1.039 ± 0.008	2.868ThrIle: 2.868 ± 0.016	2.892ThrLys: 2.892 ± 0.016	4.617ThrLeu: 4.617 ± 0.018	1.16ThrMet: 1.16 ± 0.008	2.239ThrAsn: 2.239 ± 0.011	2.584ThrPro: 2.584 ± 0.014	1.649ThrGln: 1.649 ± 0.01	2.413ThrArg: 2.413 ± 0.012	4.728ThrSer: 4.728 ± 0.018	3.062ThrThr: 3.062 ± 0.017	3.188ThrVal: 3.188 ± 0.015	0.649ThrTrp: 0.649 ± 0.006	1.433ThrTyr: 1.433 ± 0.01	0.002ThrXaa: 0.002 ± 0.0
Val	4.603ValAla: 4.603 ± 0.02	1.108ValCys: 1.108 ± 0.008	3.719ValAsp: 3.719 ± 0.015	4.531ValGlu: 4.531 ± 0.021	2.579ValPhe: 2.579 ± 0.014	3.828ValGly: 3.828 ± 0.018	1.521ValHis: 1.521 ± 0.009	3.516ValIle: 3.516 ± 0.014	4.085ValLys: 4.085 ± 0.017	6.11ValLeu: 6.11 ± 0.022	1.498ValMet: 1.498 ± 0.009	2.795ValAsn: 2.795 ± 0.012	3.147ValPro: 3.147 ± 0.017	2.419ValGln: 2.419 ± 0.012	3.006ValArg: 3.006 ± 0.015	5.208ValSer: 5.208 ± 0.019	3.217ValThr: 3.217 ± 0.015	4.756ValVal: 4.756 ± 0.019	0.728ValTrp: 0.728 ± 0.006	1.901ValTyr: 1.901 ± 0.012	0.002ValXaa: 0.002 ± 0.0
Trp	0.745TrpAla: 0.745 ± 0.007	0.24TrpCys: 0.24 ± 0.004	0.712TrpAsp: 0.712 ± 0.007	0.798TrpGlu: 0.798 ± 0.007	0.545TrpPhe: 0.545 ± 0.006	0.689TrpGly: 0.689 ± 0.007	0.299TrpHis: 0.299 ± 0.004	0.724TrpIle: 0.724 ± 0.007	0.995TrpLys: 0.995 ± 0.008	1.181TrpLeu: 1.181 ± 0.009	0.344TrpMet: 0.344 ± 0.005	0.751TrpAsn: 0.751 ± 0.008	0.481TrpPro: 0.481 ± 0.006	0.45TrpGln: 0.45 ± 0.005	0.818TrpArg: 0.818 ± 0.006	0.966TrpSer: 0.966 ± 0.008	0.654TrpThr: 0.654 ± 0.006	0.749TrpVal: 0.749 ± 0.006	0.249TrpTrp: 0.249 ± 0.004	0.361TrpTyr: 0.361 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.823TyrAla: 1.823 ± 0.011	0.664TyrCys: 0.664 ± 0.007	1.565TyrAsp: 1.565 ± 0.009	1.645TyrGlu: 1.645 ± 0.01	1.342TyrPhe: 1.342 ± 0.008	2.057TyrGly: 2.057 ± 0.014	0.764TyrHis: 0.764 ± 0.006	1.54TyrIle: 1.54 ± 0.01	1.696TyrLys: 1.696 ± 0.013	2.883TyrLeu: 2.883 ± 0.016	0.755TyrMet: 0.755 ± 0.006	1.417TyrAsn: 1.417 ± 0.011	1.267TyrPro: 1.267 ± 0.01	1.027TyrGln: 1.027 ± 0.008	1.513TyrArg: 1.513 ± 0.01	2.362TyrSer: 2.362 ± 0.013	1.329TyrThr: 1.329 ± 0.011	1.762TyrVal: 1.762 ± 0.01	0.417TyrTrp: 0.417 ± 0.005	1.077TyrTyr: 1.077 ± 0.012	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.002XaaAsp: 0.002 ± 0.0	0.002XaaGlu: 0.002 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.003XaaGly: 0.003 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.002XaaIle: 0.002 ± 0.0	0.002XaaLys: 0.002 ± 0.0	0.003XaaLeu: 0.003 ± 0.001	0.002XaaMet: 0.002 ± 0.0	0.002XaaAsn: 0.002 ± 0.0	0.002XaaPro: 0.002 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.002XaaArg: 0.002 ± 0.0	0.002XaaSer: 0.002 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.002XaaVal: 0.002 ± 0.0	0.001XaaTrp: 0.001 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 41327 proteins (17030923 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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