Homoserinibacter sp. GY 40078

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Homoserinibacter; unclassified Homoserinibacter

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2789 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C8IE42|A0A5C8IE42_9MICO SAM-dependent methyltransferase OS=Homoserinibacter sp. GY 40078 OX=2603275 GN=FVQ89_12680 PE=4 SV=1
MM1 pKa = 7.35ARR3 pKa = 11.84VPEE6 pKa = 4.28PEE8 pKa = 4.09MTPEE12 pKa = 4.31GPAYY16 pKa = 9.92EE17 pKa = 4.59GRR19 pKa = 11.84LLDD22 pKa = 3.95RR23 pKa = 11.84ADD25 pKa = 4.13EE26 pKa = 4.22EE27 pKa = 5.16VVDD30 pKa = 4.38QGASFDD36 pKa = 3.46IRR38 pKa = 11.84TLLTRR43 pKa = 11.84RR44 pKa = 11.84NVLGLGILGVGAATLVACAPTSDD67 pKa = 4.05SSTSSSSSGSTSSGSTGTTDD87 pKa = 4.36ASGLPSGEE95 pKa = 4.46IPDD98 pKa = 4.11EE99 pKa = 4.17TAGPYY104 pKa = 10.2PGDD107 pKa = 3.77GSNGPDD113 pKa = 3.17VLEE116 pKa = 3.67QSGIVRR122 pKa = 11.84SDD124 pKa = 2.66IRR126 pKa = 11.84SSIDD130 pKa = 2.91GGATAEE136 pKa = 4.13GVPLEE141 pKa = 4.23FSLTITDD148 pKa = 4.05MANDD152 pKa = 4.02DD153 pKa = 4.06APFEE157 pKa = 4.35GVAVYY162 pKa = 9.79AWHH165 pKa = 7.55CDD167 pKa = 3.19AQGRR171 pKa = 11.84YY172 pKa = 8.61SMYY175 pKa = 10.6SEE177 pKa = 4.12GVEE180 pKa = 4.0DD181 pKa = 3.48EE182 pKa = 4.49TYY184 pKa = 11.07LRR186 pKa = 11.84GVQVADD192 pKa = 3.66EE193 pKa = 4.49NGVVTFTSIFPACYY207 pKa = 8.34TGRR210 pKa = 11.84WPHH213 pKa = 5.89IHH215 pKa = 6.43FEE217 pKa = 4.33VYY219 pKa = 10.15PDD221 pKa = 4.25LDD223 pKa = 4.48SISDD227 pKa = 3.73STNAIATSQIAMPEE241 pKa = 4.2DD242 pKa = 3.7VCNTVYY248 pKa = 11.07ALSAYY253 pKa = 10.14DD254 pKa = 3.76GSSSNLSQVSLDD266 pKa = 3.33TDD268 pKa = 3.58NVFGEE273 pKa = 4.85DD274 pKa = 3.69GGALQLATVSGDD286 pKa = 2.98VDD288 pKa = 3.53SGYY291 pKa = 8.65TASLVARR298 pKa = 11.84VDD300 pKa = 3.55TTTTPSSGDD309 pKa = 3.41APSGGGQGGGQGDD322 pKa = 4.65TPPGDD327 pKa = 3.56APSRR331 pKa = 3.78

Molecular weight: 33.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C8IEX6|A0A5C8IEX6_9MICO NAD(P)/FAD-dependent oxidoreductase OS=Homoserinibacter sp. GY 40078 OX=2603275 GN=FVQ89_01910 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2789

0

2789

918376

25

2785

329.3

35.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.429 ± 0.068

0.454 ± 0.009

6.318 ± 0.041

5.879 ± 0.044

3.099 ± 0.027

9.029 ± 0.041

1.896 ± 0.019

4.588 ± 0.031

1.693 ± 0.034

10.247 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.676 ± 0.018

1.81 ± 0.024

5.47 ± 0.032

2.561 ± 0.024

7.558 ± 0.058

5.717 ± 0.043

5.885 ± 0.049

9.14 ± 0.046

1.554 ± 0.019

1.997 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski