Streptomyces sp. NRRL B-3648

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7028 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M8WRK7|A0A0M8WRK7_9ACTN Transcriptional regulator OS=Streptomyces sp. NRRL B-3648 OX=1519493 GN=ADL04_34995 PE=4 SV=1
MM1 pKa = 7.41TVQQEE6 pKa = 4.49APGGEE11 pKa = 4.07ALEE14 pKa = 4.15VWIDD18 pKa = 3.41QDD20 pKa = 3.99LCTGDD25 pKa = 5.01GICAQYY31 pKa = 10.9APEE34 pKa = 4.23VFEE37 pKa = 5.9LDD39 pKa = 3.05IDD41 pKa = 3.65GLAYY45 pKa = 10.7VKK47 pKa = 10.83GADD50 pKa = 4.87DD51 pKa = 4.98EE52 pKa = 5.3LLQAPGATTPVPLPLLTDD70 pKa = 3.6VVDD73 pKa = 4.12SAKK76 pKa = 10.15EE77 pKa = 3.93CPGEE81 pKa = 4.38CIHH84 pKa = 6.24VRR86 pKa = 11.84RR87 pKa = 11.84VSDD90 pKa = 3.3RR91 pKa = 11.84VEE93 pKa = 4.16VYY95 pKa = 10.89GPDD98 pKa = 3.45AEE100 pKa = 4.31

Molecular weight: 10.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M8WVM9|A0A0M8WVM9_9ACTN Lipoprotein OS=Streptomyces sp. NRRL B-3648 OX=1519493 GN=ADL04_27145 PE=4 SV=1
MM1 pKa = 7.37RR2 pKa = 11.84PWWRR6 pKa = 11.84TAPPRR11 pKa = 11.84SRR13 pKa = 11.84PTANATGRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84GSGGRR31 pKa = 11.84RR32 pKa = 11.84WNGPCAGGWPASWRR46 pKa = 11.84CPHH49 pKa = 6.65WSRR52 pKa = 11.84RR53 pKa = 11.84CRR55 pKa = 11.84GSATPAAVPGSPISATGPRR74 pKa = 11.84TPWWWTAGWTSSPATGHH91 pKa = 5.9RR92 pKa = 11.84AGWRR96 pKa = 11.84RR97 pKa = 11.84CCGTSAGRR105 pKa = 11.84TSATGARR112 pKa = 11.84ARR114 pKa = 11.84TPASAAGRR122 pKa = 11.84GTPWSTSGTGAGGRR136 pKa = 11.84RR137 pKa = 11.84AAHH140 pKa = 6.19AGARR144 pKa = 11.84AGRR147 pKa = 11.84SVAHH151 pKa = 6.54RR152 pKa = 11.84AVGRR156 pKa = 11.84PVAPRR161 pKa = 11.84RR162 pKa = 11.84ADD164 pKa = 3.44PGAPGVPHH172 pKa = 6.1VRR174 pKa = 11.84VAGALARR181 pKa = 11.84PGPAVPAGARR191 pKa = 11.84PGHH194 pKa = 6.07RR195 pKa = 11.84RR196 pKa = 11.84QGAGPSRR203 pKa = 11.84HH204 pKa = 5.72RR205 pKa = 11.84LLRR208 pKa = 11.84HH209 pKa = 4.9VQDD212 pKa = 4.36PGVPDD217 pKa = 4.54ALPGGVAARR226 pKa = 11.84TVVSAAGRR234 pKa = 11.84AVRR237 pKa = 11.84PP238 pKa = 3.83

Molecular weight: 24.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7028

0

7028

2248530

21

6644

319.9

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.836 ± 0.048

0.813 ± 0.008

5.912 ± 0.022

5.557 ± 0.03

2.709 ± 0.018

9.659 ± 0.033

2.389 ± 0.014

2.912 ± 0.021

2.028 ± 0.025

10.46 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.641 ± 0.012

1.66 ± 0.017

6.211 ± 0.027

2.731 ± 0.019

8.318 ± 0.037

4.776 ± 0.025

6.152 ± 0.026

8.56 ± 0.026

1.537 ± 0.012

2.141 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski