Amino acid dipepetide frequency for Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.0AlaAla: 6.0 ± 0.021	1.248AlaCys: 1.248 ± 0.007	3.076AlaAsp: 3.076 ± 0.011	4.169AlaGlu: 4.169 ± 0.014	2.726AlaPhe: 2.726 ± 0.01	3.851AlaGly: 3.851 ± 0.013	1.252AlaHis: 1.252 ± 0.008	3.749AlaIle: 3.749 ± 0.013	3.922AlaLys: 3.922 ± 0.012	6.502AlaLeu: 6.502 ± 0.018	1.767AlaMet: 1.767 ± 0.008	2.639AlaAsn: 2.639 ± 0.01	2.76AlaPro: 2.76 ± 0.012	2.078AlaGln: 2.078 ± 0.009	3.23AlaArg: 3.23 ± 0.012	5.961AlaSer: 5.961 ± 0.02	3.571AlaThr: 3.571 ± 0.012	4.658AlaVal: 4.658 ± 0.013	0.746AlaTrp: 0.746 ± 0.005	1.78AlaTyr: 1.78 ± 0.008	0.002AlaXaa: 0.002 ± 0.0
Cys	0.959CysAla: 0.959 ± 0.005	0.553CysCys: 0.553 ± 0.005	0.891CysAsp: 0.891 ± 0.007	0.943CysGlu: 0.943 ± 0.005	0.945CysPhe: 0.945 ± 0.006	1.366CysGly: 1.366 ± 0.007	0.467CysHis: 0.467 ± 0.004	1.02CysIle: 1.02 ± 0.005	1.215CysLys: 1.215 ± 0.007	1.963CysLeu: 1.963 ± 0.008	0.46CysMet: 0.46 ± 0.004	0.937CysAsn: 0.937 ± 0.006	0.943CysPro: 0.943 ± 0.006	0.647CysGln: 0.647 ± 0.005	1.059CysArg: 1.059 ± 0.006	1.89CysSer: 1.89 ± 0.009	0.839CysThr: 0.839 ± 0.005	1.019CysVal: 1.019 ± 0.006	0.265CysTrp: 0.265 ± 0.003	0.567CysTyr: 0.567 ± 0.004	0.0CysXaa: 0.0 ± 0.0
Asp	3.417AspAla: 3.417 ± 0.012	1.02AspCys: 1.02 ± 0.006	3.697AspAsp: 3.697 ± 0.016	4.015AspGlu: 4.015 ± 0.013	2.334AspPhe: 2.334 ± 0.008	3.738AspGly: 3.738 ± 0.011	1.225AspHis: 1.225 ± 0.006	3.127AspIle: 3.127 ± 0.011	2.802AspLys: 2.802 ± 0.01	5.129AspLeu: 5.129 ± 0.015	1.318AspMet: 1.318 ± 0.007	2.208AspAsn: 2.208 ± 0.009	2.547AspPro: 2.547 ± 0.01	1.753AspGln: 1.753 ± 0.008	2.373AspArg: 2.373 ± 0.01	4.252AspSer: 4.252 ± 0.015	2.154AspThr: 2.154 ± 0.008	3.604AspVal: 3.604 ± 0.01	0.713AspTrp: 0.713 ± 0.005	1.532AspTyr: 1.532 ± 0.006	0.001AspXaa: 0.001 ± 0.0
Glu	4.752GluAla: 4.752 ± 0.015	0.956GluCys: 0.956 ± 0.006	3.973GluAsp: 3.973 ± 0.014	6.16GluGlu: 6.16 ± 0.029	2.457GluPhe: 2.457 ± 0.009	3.661GluGly: 3.661 ± 0.012	1.258GluHis: 1.258 ± 0.007	3.81GluIle: 3.81 ± 0.012	4.901GluLys: 4.901 ± 0.02	6.085GluLeu: 6.085 ± 0.016	1.803GluMet: 1.803 ± 0.008	3.294GluAsn: 3.294 ± 0.013	2.188GluPro: 2.188 ± 0.01	2.266GluGln: 2.266 ± 0.01	3.371GluArg: 3.371 ± 0.013	4.632GluSer: 4.632 ± 0.014	3.229GluThr: 3.229 ± 0.013	4.187GluVal: 4.187 ± 0.012	0.74GluTrp: 0.74 ± 0.005	1.652GluTyr: 1.652 ± 0.007	0.002GluXaa: 0.002 ± 0.0
Phe	2.424PheAla: 2.424 ± 0.01	0.93PheCys: 0.93 ± 0.006	2.385PheAsp: 2.385 ± 0.008	2.321PheGlu: 2.321 ± 0.009	1.96PhePhe: 1.96 ± 0.009	3.026PheGly: 3.026 ± 0.014	1.146PheHis: 1.146 ± 0.007	2.152PheIle: 2.152 ± 0.009	2.221PheLys: 2.221 ± 0.009	4.311PheLeu: 4.311 ± 0.015	0.991PheMet: 0.991 ± 0.006	1.877PheAsn: 1.877 ± 0.007	2.161PhePro: 2.161 ± 0.009	1.631PheGln: 1.631 ± 0.006	2.034PheArg: 2.034 ± 0.008	4.147PheSer: 4.147 ± 0.012	2.002PheThr: 2.002 ± 0.009	2.653PheVal: 2.653 ± 0.009	0.573PheTrp: 0.573 ± 0.005	1.281PheTyr: 1.281 ± 0.008	0.001PheXaa: 0.001 ± 0.0
Gly	3.709GlyAla: 3.709 ± 0.014	1.323GlyCys: 1.323 ± 0.008	3.32GlyAsp: 3.32 ± 0.01	3.625GlyGlu: 3.625 ± 0.011	3.141GlyPhe: 3.141 ± 0.011	5.008GlyGly: 5.008 ± 0.026	1.535GlyHis: 1.535 ± 0.008	3.634GlyIle: 3.634 ± 0.01	4.17GlyLys: 4.17 ± 0.014	5.813GlyLeu: 5.813 ± 0.014	1.491GlyMet: 1.491 ± 0.009	3.328GlyAsn: 3.328 ± 0.012	2.375GlyPro: 2.375 ± 0.01	2.088GlyGln: 2.088 ± 0.01	3.466GlyArg: 3.466 ± 0.014	6.166GlySer: 6.166 ± 0.022	3.254GlyThr: 3.254 ± 0.01	3.968GlyVal: 3.968 ± 0.012	0.871GlyTrp: 0.871 ± 0.006	2.042GlyTyr: 2.042 ± 0.009	0.002GlyXaa: 0.002 ± 0.0
His	1.343HisAla: 1.343 ± 0.007	0.557HisCys: 0.557 ± 0.005	1.143HisAsp: 1.143 ± 0.006	1.287HisGlu: 1.287 ± 0.007	1.101HisPhe: 1.101 ± 0.005	1.699HisGly: 1.699 ± 0.008	0.954HisHis: 0.954 ± 0.008	1.212HisIle: 1.212 ± 0.006	1.198HisLys: 1.198 ± 0.007	2.465HisLeu: 2.465 ± 0.01	0.54HisMet: 0.54 ± 0.004	0.966HisAsn: 0.966 ± 0.005	1.404HisPro: 1.404 ± 0.008	1.06HisGln: 1.06 ± 0.006	1.339HisArg: 1.339 ± 0.006	1.967HisSer: 1.967 ± 0.009	0.909HisThr: 0.909 ± 0.006	1.528HisVal: 1.528 ± 0.007	0.306HisTrp: 0.306 ± 0.003	0.701HisTyr: 0.701 ± 0.004	0.001HisXaa: 0.001 ± 0.0
Ile	3.554IleAla: 3.554 ± 0.011	1.15IleCys: 1.15 ± 0.007	2.971IleAsp: 2.971 ± 0.008	3.38IleGlu: 3.38 ± 0.012	2.277IlePhe: 2.277 ± 0.009	3.402IleGly: 3.402 ± 0.012	1.284IleHis: 1.284 ± 0.006	2.81IleIle: 2.81 ± 0.01	3.069IleLys: 3.069 ± 0.01	5.167IleLeu: 5.167 ± 0.015	1.143IleMet: 1.143 ± 0.006	2.307IleAsn: 2.307 ± 0.008	2.996IlePro: 2.996 ± 0.013	2.027IleGln: 2.027 ± 0.008	2.639IleArg: 2.639 ± 0.011	4.945IleSer: 4.945 ± 0.013	2.553IleThr: 2.553 ± 0.009	3.415IleVal: 3.415 ± 0.01	0.718IleTrp: 0.718 ± 0.004	1.461IleTyr: 1.461 ± 0.007	0.001IleXaa: 0.001 ± 0.0
Lys	4.224LysAla: 4.224 ± 0.013	1.033LysCys: 1.033 ± 0.007	3.343LysAsp: 3.343 ± 0.011	4.849LysGlu: 4.849 ± 0.019	2.234LysPhe: 2.234 ± 0.009	3.781LysGly: 3.781 ± 0.011	1.438LysHis: 1.438 ± 0.008	3.295LysIle: 3.295 ± 0.011	4.868LysLys: 4.868 ± 0.022	6.268LysLeu: 6.268 ± 0.018	1.543LysMet: 1.543 ± 0.007	2.746LysAsn: 2.746 ± 0.01	2.864LysPro: 2.864 ± 0.011	2.504LysGln: 2.504 ± 0.01	3.654LysArg: 3.654 ± 0.012	4.731LysSer: 4.731 ± 0.014	2.958LysThr: 2.958 ± 0.011	3.998LysVal: 3.998 ± 0.011	0.804LysTrp: 0.804 ± 0.005	1.643LysTyr: 1.643 ± 0.008	0.001LysXaa: 0.001 ± 0.0
Leu	6.316LeuAla: 6.316 ± 0.017	1.838LeuCys: 1.838 ± 0.008	5.151LeuAsp: 5.151 ± 0.016	6.546LeuGlu: 6.546 ± 0.02	3.826LeuPhe: 3.826 ± 0.012	5.687LeuGly: 5.687 ± 0.014	2.571LeuHis: 2.571 ± 0.009	4.603LeuIle: 4.603 ± 0.015	6.562LeuLys: 6.562 ± 0.019	9.798LeuLeu: 9.798 ± 0.027	2.179LeuMet: 2.179 ± 0.007	4.213LeuAsn: 4.213 ± 0.013	5.198LeuPro: 5.198 ± 0.014	4.506LeuGln: 4.506 ± 0.016	5.422LeuArg: 5.422 ± 0.017	8.824LeuSer: 8.824 ± 0.027	4.476LeuThr: 4.476 ± 0.014	6.249LeuVal: 6.249 ± 0.015	1.141LeuTrp: 1.141 ± 0.006	2.504LeuTyr: 2.504 ± 0.009	0.003LeuXaa: 0.003 ± 0.0
Met	2.1MetAla: 2.1 ± 0.009	0.324MetCys: 0.324 ± 0.003	1.444MetAsp: 1.444 ± 0.006	2.071MetGlu: 2.071 ± 0.008	0.838MetPhe: 0.838 ± 0.005	1.646MetGly: 1.646 ± 0.009	0.554MetHis: 0.554 ± 0.004	1.187MetIle: 1.187 ± 0.007	1.685MetLys: 1.685 ± 0.007	2.225MetLeu: 2.225 ± 0.009	0.678MetMet: 0.678 ± 0.005	1.066MetAsn: 1.066 ± 0.007	1.076MetPro: 1.076 ± 0.006	0.98MetGln: 0.98 ± 0.007	1.156MetArg: 1.156 ± 0.006	1.763MetSer: 1.763 ± 0.008	1.009MetThr: 1.009 ± 0.006	1.701MetVal: 1.701 ± 0.007	0.245MetTrp: 0.245 ± 0.003	0.558MetTyr: 0.558 ± 0.004	0.0MetXaa: 0.0 ± 0.0
Asn	2.753AsnAla: 2.753 ± 0.009	0.92AsnCys: 0.92 ± 0.006	2.204AsnAsp: 2.204 ± 0.008	2.761AsnGlu: 2.761 ± 0.011	1.98AsnPhe: 1.98 ± 0.009	3.503AsnGly: 3.503 ± 0.013	1.162AsnHis: 1.162 ± 0.007	2.533AsnIle: 2.533 ± 0.008	2.581AsnLys: 2.581 ± 0.01	4.879AsnLeu: 4.879 ± 0.019	1.164AsnMet: 1.164 ± 0.006	2.472AsnAsn: 2.472 ± 0.012	2.411AsnPro: 2.411 ± 0.011	1.887AsnGln: 1.887 ± 0.008	2.088AsnArg: 2.088 ± 0.008	4.025AsnSer: 4.025 ± 0.013	1.978AsnThr: 1.978 ± 0.009	2.851AsnVal: 2.851 ± 0.01	0.563AsnTrp: 0.563 ± 0.004	1.305AsnTyr: 1.305 ± 0.007	0.001AsnXaa: 0.001 ± 0.0
Pro	2.904ProAla: 2.904 ± 0.012	0.802ProCys: 0.802 ± 0.005	2.424ProAsp: 2.424 ± 0.009	3.089ProGlu: 3.089 ± 0.012	2.067ProPhe: 2.067 ± 0.008	2.645ProGly: 2.645 ± 0.013	1.068ProHis: 1.068 ± 0.006	2.356ProIle: 2.356 ± 0.01	2.871ProLys: 2.871 ± 0.013	4.394ProLeu: 4.394 ± 0.014	1.02ProMet: 1.02 ± 0.006	2.352ProAsn: 2.352 ± 0.01	3.799ProPro: 3.799 ± 0.034	1.859ProGln: 1.859 ± 0.009	2.368ProArg: 2.368 ± 0.01	5.213ProSer: 5.213 ± 0.016	2.665ProThr: 2.665 ± 0.011	3.094ProVal: 3.094 ± 0.012	0.61ProTrp: 0.61 ± 0.004	1.347ProTyr: 1.347 ± 0.008	0.002ProXaa: 0.002 ± 0.0
Gln	2.454GlnAla: 2.454 ± 0.009	0.614GlnCys: 0.614 ± 0.004	1.669GlnAsp: 1.669 ± 0.006	2.418GlnGlu: 2.418 ± 0.01	1.433GlnPhe: 1.433 ± 0.007	2.206GlnGly: 2.206 ± 0.009	0.992GlnHis: 0.992 ± 0.006	2.079GlnIle: 2.079 ± 0.008	2.381GlnLys: 2.381 ± 0.009	3.851GlnLeu: 3.851 ± 0.014	1.018GlnMet: 1.018 ± 0.006	1.88GlnAsn: 1.88 ± 0.009	1.837GlnPro: 1.837 ± 0.009	2.323GlnGln: 2.323 ± 0.024	2.148GlnArg: 2.148 ± 0.009	3.004GlnSer: 3.004 ± 0.013	1.757GlnThr: 1.757 ± 0.009	2.41GlnVal: 2.41 ± 0.008	0.484GlnTrp: 0.484 ± 0.004	0.986GlnTyr: 0.986 ± 0.006	0.001GlnXaa: 0.001 ± 0.0
Arg	3.109ArgAla: 3.109 ± 0.012	1.008ArgCys: 1.008 ± 0.006	2.643ArgAsp: 2.643 ± 0.011	3.28ArgGlu: 3.28 ± 0.011	2.222ArgPhe: 2.222 ± 0.009	3.09ArgGly: 3.09 ± 0.013	1.249ArgHis: 1.249 ± 0.007	2.846ArgIle: 2.846 ± 0.01	3.84ArgLys: 3.84 ± 0.013	5.007ArgLeu: 5.007 ± 0.014	1.286ArgMet: 1.286 ± 0.006	2.496ArgAsn: 2.496 ± 0.008	2.287ArgPro: 2.287 ± 0.01	1.917ArgGln: 1.917 ± 0.008	3.749ArgArg: 3.749 ± 0.013	4.332ArgSer: 4.332 ± 0.015	2.439ArgThr: 2.439 ± 0.009	3.173ArgVal: 3.173 ± 0.01	0.712ArgTrp: 0.712 ± 0.005	1.475ArgTyr: 1.475 ± 0.007	0.002ArgXaa: 0.002 ± 0.0
Ser	5.27SerAla: 5.27 ± 0.015	1.742SerCys: 1.742 ± 0.008	4.439SerAsp: 4.439 ± 0.014	4.876SerGlu: 4.876 ± 0.015	4.131SerPhe: 4.131 ± 0.013	5.964SerGly: 5.964 ± 0.02	2.012SerHis: 2.012 ± 0.01	4.654SerIle: 4.654 ± 0.013	5.252SerLys: 5.252 ± 0.016	8.886SerLeu: 8.886 ± 0.024	2.171SerMet: 2.171 ± 0.008	4.357SerAsn: 4.357 ± 0.014	4.569SerPro: 4.569 ± 0.018	3.124SerGln: 3.124 ± 0.011	4.423SerArg: 4.423 ± 0.015	11.377SerSer: 11.377 ± 0.043	4.789SerThr: 4.789 ± 0.015	5.162SerVal: 5.162 ± 0.014	1.135SerTrp: 1.135 ± 0.007	2.343SerTyr: 2.343 ± 0.008	0.002SerXaa: 0.002 ± 0.0
Thr	3.37ThrAla: 3.37 ± 0.012	0.931ThrCys: 0.931 ± 0.006	2.303ThrAsp: 2.303 ± 0.008	2.797ThrGlu: 2.797 ± 0.011	2.055ThrPhe: 2.055 ± 0.009	3.242ThrGly: 3.242 ± 0.01	1.007ThrHis: 1.007 ± 0.006	2.714ThrIle: 2.714 ± 0.01	2.772ThrLys: 2.772 ± 0.008	4.592ThrLeu: 4.592 ± 0.013	1.179ThrMet: 1.179 ± 0.006	2.148ThrAsn: 2.148 ± 0.01	2.573ThrPro: 2.573 ± 0.012	1.566ThrGln: 1.566 ± 0.008	2.338ThrArg: 2.338 ± 0.009	4.701ThrSer: 4.701 ± 0.013	2.936ThrThr: 2.936 ± 0.013	3.323ThrVal: 3.323 ± 0.01	0.619ThrTrp: 0.619 ± 0.004	1.336ThrTyr: 1.336 ± 0.007	0.001ThrXaa: 0.001 ± 0.0
Val	4.628ValAla: 4.628 ± 0.013	1.117ValCys: 1.117 ± 0.006	3.683ValAsp: 3.683 ± 0.011	4.403ValGlu: 4.403 ± 0.014	2.676ValPhe: 2.676 ± 0.01	4.004ValGly: 4.004 ± 0.012	1.469ValHis: 1.469 ± 0.007	3.357ValIle: 3.357 ± 0.01	3.956ValLys: 3.956 ± 0.009	6.233ValLeu: 6.233 ± 0.015	1.486ValMet: 1.486 ± 0.006	2.722ValAsn: 2.722 ± 0.01	3.21ValPro: 3.21 ± 0.011	2.298ValGln: 2.298 ± 0.008	2.976ValArg: 2.976 ± 0.01	5.517ValSer: 5.517 ± 0.014	3.155ValThr: 3.155 ± 0.01	4.662ValVal: 4.662 ± 0.013	0.74ValTrp: 0.74 ± 0.005	1.88ValTyr: 1.88 ± 0.008	0.001ValXaa: 0.001 ± 0.0
Trp	0.721TrpAla: 0.721 ± 0.005	0.24TrpCys: 0.24 ± 0.002	0.702TrpAsp: 0.702 ± 0.005	0.786TrpGlu: 0.786 ± 0.005	0.536TrpPhe: 0.536 ± 0.004	0.725TrpGly: 0.725 ± 0.006	0.299TrpHis: 0.299 ± 0.003	0.691TrpIle: 0.691 ± 0.004	0.971TrpLys: 0.971 ± 0.005	1.236TrpLeu: 1.236 ± 0.007	0.337TrpMet: 0.337 ± 0.003	0.691TrpAsn: 0.691 ± 0.005	0.486TrpPro: 0.486 ± 0.004	0.453TrpGln: 0.453 ± 0.004	0.825TrpArg: 0.825 ± 0.006	0.957TrpSer: 0.957 ± 0.006	0.605TrpThr: 0.605 ± 0.005	0.79TrpVal: 0.79 ± 0.006	0.251TrpTrp: 0.251 ± 0.003	0.345TrpTyr: 0.345 ± 0.003	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.678TyrAla: 1.678 ± 0.007	0.641TyrCys: 0.641 ± 0.005	1.495TyrAsp: 1.495 ± 0.007	1.601TyrGlu: 1.601 ± 0.007	1.283TyrPhe: 1.283 ± 0.007	2.082TyrGly: 2.082 ± 0.009	0.735TyrHis: 0.735 ± 0.005	1.441TyrIle: 1.441 ± 0.006	1.544TyrLys: 1.544 ± 0.009	2.808TyrLeu: 2.808 ± 0.009	0.734TyrMet: 0.734 ± 0.005	1.321TyrAsn: 1.321 ± 0.007	1.265TyrPro: 1.265 ± 0.007	0.976TyrGln: 0.976 ± 0.006	1.501TyrArg: 1.501 ± 0.007	2.273TyrSer: 2.273 ± 0.009	1.224TyrThr: 1.224 ± 0.007	1.727TyrVal: 1.727 ± 0.008	0.409TyrTrp: 0.409 ± 0.004	0.951TyrTyr: 0.951 ± 0.006	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.002XaaGlu: 0.002 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.002XaaGly: 0.002 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.002XaaLys: 0.002 ± 0.0	0.002XaaLeu: 0.002 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.002XaaArg: 0.002 ± 0.0	0.003XaaSer: 0.003 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.002XaaVal: 0.002 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.042XaaXaa: 0.042 ± 0.003

Statistics based on 76176 proteins (33397352 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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