Steccherinum ochraceum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Steccherinaceae; Steccherinum

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12246 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R0RHA8|A0A4R0RHA8_9APHY Uncharacterized protein OS=Steccherinum ochraceum OX=92696 GN=EIP91_004451 PE=3 SV=1
MM1 pKa = 7.44SLLSYY6 pKa = 10.11TLLALSLAGARR17 pKa = 11.84AATVSGSADD26 pKa = 3.28VNATAGASGSSVDD39 pKa = 3.7MVSAAWYY46 pKa = 7.66TGWHH50 pKa = 6.38SDD52 pKa = 2.97NFTLANVSWDD62 pKa = 3.42KK63 pKa = 9.27YY64 pKa = 7.17THH66 pKa = 5.01MTYY69 pKa = 10.72SFAITTPDD77 pKa = 3.24VKK79 pKa = 10.61QVSLDD84 pKa = 3.42GSDD87 pKa = 4.5PDD89 pKa = 4.56LLPQFVEE96 pKa = 4.27QAQKK100 pKa = 11.14NGVKK104 pKa = 10.47ALVSVGGWTGSLYY117 pKa = 10.67YY118 pKa = 10.42SSNVGSADD126 pKa = 3.03NRR128 pKa = 11.84TAFVKK133 pKa = 10.16TITDD137 pKa = 4.23FATQYY142 pKa = 11.05KK143 pKa = 10.42LDD145 pKa = 5.11GIDD148 pKa = 5.23FDD150 pKa = 4.0WEE152 pKa = 4.41YY153 pKa = 10.69PGTQGIGCNAISPDD167 pKa = 3.49DD168 pKa = 3.7TTNFLSFLQEE178 pKa = 3.85LRR180 pKa = 11.84QDD182 pKa = 3.69PVGKK186 pKa = 10.06NLFLTAATSITPWKK200 pKa = 10.23GPDD203 pKa = 3.35GTSLSDD209 pKa = 3.1VSGFAKK215 pKa = 10.49VLDD218 pKa = 4.58YY219 pKa = 10.84IAIMNYY225 pKa = 9.93DD226 pKa = 3.38INGAWSTAVGANAPLNDD243 pKa = 3.53TCAPAANQAGSAVSAVAAWTVAGMPSHH270 pKa = 6.36QIVLGVPSYY279 pKa = 10.98GHH281 pKa = 6.08SFHH284 pKa = 8.08VDD286 pKa = 2.83VEE288 pKa = 4.62DD289 pKa = 5.94AFHH292 pKa = 7.27CDD294 pKa = 5.4DD295 pKa = 4.72NGDD298 pKa = 4.05DD299 pKa = 3.99NGVLASFPPFDD310 pKa = 4.88KK311 pKa = 10.93AQQPSGDD318 pKa = 3.52AWDD321 pKa = 5.32DD322 pKa = 3.69GAGTDD327 pKa = 3.07ICGNQVAAGGNFDD340 pKa = 3.47FWGLIDD346 pKa = 5.41GGFLNANGTVADD358 pKa = 4.36GVFYY362 pKa = 10.53RR363 pKa = 11.84YY364 pKa = 9.79DD365 pKa = 3.44EE366 pKa = 4.8CSQTPYY372 pKa = 10.85VYY374 pKa = 10.91NEE376 pKa = 4.23DD377 pKa = 3.84SQVMVAFDD385 pKa = 4.29DD386 pKa = 4.07ATSFAAKK393 pKa = 10.22GDD395 pKa = 3.84FVKK398 pKa = 10.29TSNLRR403 pKa = 11.84GFAMWEE409 pKa = 3.82AGGDD413 pKa = 3.91SNDD416 pKa = 3.57ILLDD420 pKa = 3.9SIRR423 pKa = 11.84QAAGFEE429 pKa = 4.31TEE431 pKa = 6.04DD432 pKa = 5.14DD433 pKa = 4.84GEE435 pKa = 4.38DD436 pKa = 3.56CC437 pKa = 5.9

Molecular weight: 46.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R0RFQ8|A0A4R0RFQ8_9APHY F-box domain-containing protein OS=Steccherinum ochraceum OX=92696 GN=EIP91_006794 PE=4 SV=1
SS1 pKa = 7.04PLPHH5 pKa = 6.93RR6 pKa = 11.84RR7 pKa = 11.84LLLDD11 pKa = 3.71LRR13 pKa = 11.84CGSGRR18 pKa = 11.84AMILRR23 pKa = 11.84TMTGKK28 pKa = 9.82VLKK31 pKa = 8.04TTTTMLPPICSNARR45 pKa = 11.84TPHH48 pKa = 6.67PPPPPPHH55 pKa = 6.93RR56 pKa = 11.84PLPPQMRR63 pKa = 11.84PHH65 pKa = 6.59SRR67 pKa = 11.84QLPLNLGGTSGRR79 pKa = 11.84ATTRR83 pKa = 11.84ARR85 pKa = 11.84TQMSKK90 pKa = 10.3VVMIAKK96 pKa = 6.51TTLRR100 pKa = 11.84TSPTTLPNARR110 pKa = 11.84TPHH113 pKa = 6.72PPPPPPHH120 pKa = 7.0RR121 pKa = 11.84LLPPQMRR128 pKa = 11.84PHH130 pKa = 6.5SRR132 pKa = 11.84QLPINLGGISGRR144 pKa = 11.84AMTRR148 pKa = 11.84SRR150 pKa = 11.84AMAKK154 pKa = 8.72TVVMMLTVVRR164 pKa = 11.84TTAWSDD170 pKa = 3.34ARR172 pKa = 11.84PQSLSQQLPRR182 pKa = 11.84RR183 pKa = 11.84ALGGRR188 pKa = 11.84FRR190 pKa = 11.84GAMRR194 pKa = 11.84LMTRR198 pKa = 11.84ARR200 pKa = 11.84MVMMARR206 pKa = 11.84TFHH209 pKa = 6.41

Molecular weight: 23.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12246

0

12246

5899370

50

5007

481.7

53.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.861 ± 0.018

1.131 ± 0.007

5.777 ± 0.015

5.858 ± 0.019

3.721 ± 0.014

6.346 ± 0.022

2.59 ± 0.01

4.602 ± 0.016

4.396 ± 0.018

9.268 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.095 ± 0.008

3.227 ± 0.012

6.75 ± 0.028

3.743 ± 0.012

6.149 ± 0.016

8.782 ± 0.027

6.093 ± 0.013

6.577 ± 0.015

1.416 ± 0.007

2.616 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski