Yersinia phage fPS-54-ocr

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Helsettvirus; Yersinia virus fPS54ocr

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H1UJF1|A0A2H1UJF1_9CAUD Phage endopeptidase RZ1 OS=Yersinia phage fPS-54-ocr OX=2052753 GN=g042 PE=4 SV=1
MM1 pKa = 7.47SNEE4 pKa = 4.05SNADD8 pKa = 3.08VYY10 pKa = 11.65AEE12 pKa = 4.07MGANPTAVTGSMSDD26 pKa = 3.25HH27 pKa = 6.52DD28 pKa = 4.13TSMLEE33 pKa = 3.92LDD35 pKa = 3.41VDD37 pKa = 3.97TRR39 pKa = 11.84DD40 pKa = 3.24GDD42 pKa = 4.18DD43 pKa = 5.06RR44 pKa = 11.84ITLVDD49 pKa = 4.48NDD51 pKa = 4.38DD52 pKa = 4.49PYY54 pKa = 11.39NTVDD58 pKa = 3.75PFADD62 pKa = 3.88PEE64 pKa = 5.51ADD66 pKa = 3.49DD67 pKa = 3.8GTRR70 pKa = 11.84NSLRR74 pKa = 11.84ISSEE78 pKa = 3.61GNEE81 pKa = 4.94DD82 pKa = 3.17IDD84 pKa = 4.47SGEE87 pKa = 4.24GEE89 pKa = 4.06PDD91 pKa = 3.05EE92 pKa = 4.65SQSEE96 pKa = 4.17EE97 pKa = 3.93ATDD100 pKa = 4.47FEE102 pKa = 6.3AIGDD106 pKa = 4.04APSEE110 pKa = 4.06LTEE113 pKa = 4.56ASAQLEE119 pKa = 4.08EE120 pKa = 4.9HH121 pKa = 6.18EE122 pKa = 5.51AGFQEE127 pKa = 4.63MVDD130 pKa = 3.49AAAEE134 pKa = 4.11RR135 pKa = 11.84GLSDD139 pKa = 4.66DD140 pKa = 4.94AITRR144 pKa = 11.84IQSEE148 pKa = 4.46YY149 pKa = 10.69QGDD152 pKa = 4.14GLTKK156 pKa = 10.32EE157 pKa = 4.52SYY159 pKa = 10.72EE160 pKa = 3.99EE161 pKa = 3.79LAAAGYY167 pKa = 9.99SKK169 pKa = 11.12SFVDD173 pKa = 3.98SYY175 pKa = 11.69IRR177 pKa = 11.84GQEE180 pKa = 3.98ALVEE184 pKa = 4.54SYY186 pKa = 10.87VKK188 pKa = 10.61SVFAYY193 pKa = 10.29AGGEE197 pKa = 3.99QQFNALYY204 pKa = 9.04THH206 pKa = 7.16LEE208 pKa = 3.96ATNAEE213 pKa = 4.25AAQSLVSALEE223 pKa = 3.9SRR225 pKa = 11.84DD226 pKa = 3.44LSTVKK231 pKa = 10.73AIVNLAGASRR241 pKa = 11.84AKK243 pKa = 10.23TFGKK247 pKa = 9.29PAARR251 pKa = 11.84SVTKK255 pKa = 9.17QARR258 pKa = 11.84QSAPVSNKK266 pKa = 8.71VVGYY270 pKa = 8.48TNSADD275 pKa = 3.39MMKK278 pKa = 10.98DD279 pKa = 3.14MNDD282 pKa = 3.06PRR284 pKa = 11.84YY285 pKa = 9.39STDD288 pKa = 2.59AKK290 pKa = 10.31FRR292 pKa = 11.84KK293 pKa = 9.26EE294 pKa = 4.06VEE296 pKa = 4.2LKK298 pKa = 10.05TMYY301 pKa = 9.18STYY304 pKa = 11.44

Molecular weight: 32.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H1UJF4|A0A2H1UJF4_9CAUD Uncharacterized protein OS=Yersinia phage fPS-54-ocr OX=2052753 GN=g045 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84KK3 pKa = 9.46SYY5 pKa = 10.37KK6 pKa = 9.87QFYY9 pKa = 8.47KK10 pKa = 10.46QPRR13 pKa = 11.84NHH15 pKa = 5.49IQVWVSANGPIPSGYY30 pKa = 10.51YY31 pKa = 9.08IDD33 pKa = 5.89HH34 pKa = 7.39IDD36 pKa = 4.66GNPLNDD42 pKa = 3.55NLDD45 pKa = 3.94NLRR48 pKa = 11.84LATPSEE54 pKa = 4.02NSRR57 pKa = 11.84NMKK60 pKa = 9.28TPSSNITGLKK70 pKa = 7.7WLSWCPLRR78 pKa = 11.84LTWRR82 pKa = 11.84GTLKK86 pKa = 10.8VNYY89 pKa = 8.48KK90 pKa = 7.42QHH92 pKa = 6.43SYY94 pKa = 10.55RR95 pKa = 11.84SKK97 pKa = 11.16DD98 pKa = 3.43LFDD101 pKa = 3.6VVSWLFRR108 pKa = 11.84TRR110 pKa = 11.84RR111 pKa = 11.84EE112 pKa = 3.5IHH114 pKa = 5.83GQFARR119 pKa = 11.84NRR121 pKa = 3.65

Molecular weight: 14.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11936

41

1317

248.7

27.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.638 ± 0.483

0.938 ± 0.143

6.334 ± 0.274

6.744 ± 0.282

3.452 ± 0.171

7.666 ± 0.252

1.785 ± 0.176

5.312 ± 0.225

6.82 ± 0.365

8.068 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.983 ± 0.176

4.708 ± 0.266

3.544 ± 0.225

3.795 ± 0.25

5.546 ± 0.198

6.409 ± 0.324

5.747 ± 0.196

6.635 ± 0.252

1.424 ± 0.165

3.452 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski