Hydrocarboniphaga effusa AP103

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Nevskiales; Sinobacteraceae; Hydrocarboniphaga; Hydrocarboniphaga effusa

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4474 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I8T5L1|I8T5L1_9GAMM LPS-assembly protein LptD OS=Hydrocarboniphaga effusa AP103 OX=1172194 GN=lptD PE=3 SV=1
MM1 pKa = 6.74RR2 pKa = 11.84TEE4 pKa = 4.5RR5 pKa = 11.84FCALSLVGALLCGCEE20 pKa = 4.13ASTSIDD26 pKa = 3.54FASAGYY32 pKa = 9.62AQADD36 pKa = 3.81QIMLAVEE43 pKa = 4.72GVDD46 pKa = 4.43LLDD49 pKa = 5.15DD50 pKa = 4.52GGSEE54 pKa = 4.94HH55 pKa = 6.97EE56 pKa = 4.99LDD58 pKa = 3.96SDD60 pKa = 4.06ADD62 pKa = 3.85SSFDD66 pKa = 3.82ALDD69 pKa = 3.54YY70 pKa = 11.6SNTDD74 pKa = 2.92TLQLVDD80 pKa = 4.21GTDD83 pKa = 3.52VAQGRR88 pKa = 11.84YY89 pKa = 7.26TGLRR93 pKa = 11.84LRR95 pKa = 11.84FSNEE99 pKa = 2.61GSALYY104 pKa = 10.06TSDD107 pKa = 2.97GAAYY111 pKa = 9.12PIDD114 pKa = 3.72IGSNLTFADD123 pKa = 3.25MDD125 pKa = 3.69MDD127 pKa = 4.43LDD129 pKa = 4.04EE130 pKa = 5.64GEE132 pKa = 4.25SGSRR136 pKa = 11.84LVIFEE141 pKa = 4.7PRR143 pKa = 11.84FSLQASTTTDD153 pKa = 2.36GHH155 pKa = 5.53YY156 pKa = 9.91TLTPVLRR163 pKa = 11.84VVHH166 pKa = 6.89PDD168 pKa = 2.91RR169 pKa = 11.84AASITGSVDD178 pKa = 3.35DD179 pKa = 4.17EE180 pKa = 4.49LVRR183 pKa = 11.84SSACRR188 pKa = 11.84QSRR191 pKa = 11.84TLGSGVAVYY200 pKa = 10.73AFVGANVTPQDD211 pKa = 3.82YY212 pKa = 10.21QRR214 pKa = 11.84SSGASPVAAADD225 pKa = 3.65VKK227 pKa = 10.46TGDD230 pKa = 4.5DD231 pKa = 3.77GASFYY236 pKa = 11.49YY237 pKa = 10.41DD238 pKa = 3.91FPYY241 pKa = 9.62LTAGTYY247 pKa = 8.8TLAVTCNADD256 pKa = 3.35SEE258 pKa = 4.78NPGRR262 pKa = 11.84NDD264 pKa = 3.1GLGFLAQSSVTVEE277 pKa = 4.06EE278 pKa = 4.8NEE280 pKa = 4.28SQTVNLAEE288 pKa = 4.03

Molecular weight: 30.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I8TD70|I8TD70_9GAMM Uncharacterized protein OS=Hydrocarboniphaga effusa AP103 OX=1172194 GN=WQQ_20670 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.38RR3 pKa = 11.84TYY5 pKa = 9.98QPHH8 pKa = 5.24SLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 7.45RR14 pKa = 11.84THH16 pKa = 6.03GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.32GRR39 pKa = 11.84ARR41 pKa = 11.84LTPVV45 pKa = 2.71

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4474

0

4474

1483553

37

3837

331.6

36.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.144 ± 0.043

0.882 ± 0.012

5.608 ± 0.024

5.631 ± 0.035

3.505 ± 0.02

8.316 ± 0.041

2.063 ± 0.018

4.636 ± 0.025

3.219 ± 0.033

10.809 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.102 ± 0.019

2.685 ± 0.024

5.145 ± 0.023

3.821 ± 0.024

7.431 ± 0.043

6.024 ± 0.033

4.953 ± 0.028

7.138 ± 0.031

1.405 ± 0.015

2.482 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski