Lactococcus phage BM13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vedamuthuvirus; Lactococcus virus BM13

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9QMA2|R9QMA2_9CAUD Uncharacterized protein OS=Lactococcus phage BM13 OX=1229751 GN=BM13_33 PE=4 SV=1
MM1 pKa = 7.35TDD3 pKa = 3.24KK4 pKa = 11.17LISLVNDD11 pKa = 3.12WRR13 pKa = 11.84GGFYY17 pKa = 10.77LMPEE21 pKa = 4.1DD22 pKa = 4.04TPEE25 pKa = 3.89EE26 pKa = 3.99EE27 pKa = 4.03LRR29 pKa = 11.84EE30 pKa = 4.04VEE32 pKa = 5.18VYY34 pKa = 10.96CDD36 pKa = 3.23TCGDD40 pKa = 3.54NDD42 pKa = 4.7SIIGQFSNWNQLKK55 pKa = 10.45KK56 pKa = 11.08EE57 pKa = 4.06MTDD60 pKa = 3.5DD61 pKa = 4.56EE62 pKa = 5.2GWCPYY67 pKa = 10.28SDD69 pKa = 5.14EE70 pKa = 4.2YY71 pKa = 10.46LQSVFEE77 pKa = 4.85EE78 pKa = 4.86DD79 pKa = 3.67DD80 pKa = 3.23QQ81 pKa = 6.2

Molecular weight: 9.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9QMT7|R9QMT7_9CAUD Putative anti-repressor OS=Lactococcus phage BM13 OX=1229751 GN=BM13_02 PE=4 SV=1
MM1 pKa = 7.72LFQIQIQITKK11 pKa = 10.36CFGRR15 pKa = 11.84DD16 pKa = 2.96KK17 pKa = 11.44APFSKK22 pKa = 10.51ARR24 pKa = 11.84ALFLCYY30 pKa = 10.22NIVGMFVRR38 pKa = 11.84FHH40 pKa = 7.12PIPRR44 pKa = 11.84VKK46 pKa = 10.41PFFGVAFFVCCKK58 pKa = 8.66FTTILSEE65 pKa = 4.18TT66 pKa = 3.81

Molecular weight: 7.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

9662

44

733

175.7

19.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.531 ± 0.649

0.486 ± 0.093

6.2 ± 0.411

7.235 ± 0.625

4.782 ± 0.279

6.645 ± 0.58

1.283 ± 0.166

7.162 ± 0.346

9.025 ± 0.533

7.617 ± 0.38

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.339 ± 0.155

6.52 ± 0.37

2.66 ± 0.179

4.73 ± 0.384

3.767 ± 0.293

5.961 ± 0.275

6.034 ± 0.393

5.951 ± 0.274

1.19 ± 0.123

3.881 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski