Helicobacter phage Pt1293U

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; unclassified Schmidvirus

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1S5RGS6|A0A1S5RGS6_9CAUD Structural protein OS=Helicobacter phage Pt1293U OX=1852669 PE=4 SV=1
MM1 pKa = 7.66GIKK4 pKa = 9.82EE5 pKa = 4.23KK6 pKa = 10.55EE7 pKa = 3.99IEE9 pKa = 4.18LEE11 pKa = 3.99TLKK14 pKa = 11.01RR15 pKa = 11.84EE16 pKa = 3.99IAQAEE21 pKa = 4.06ASLEE25 pKa = 3.89QDD27 pKa = 4.59FIKK30 pKa = 10.97HH31 pKa = 5.29MVDD34 pKa = 2.81KK35 pKa = 10.6TNEE38 pKa = 3.95KK39 pKa = 10.6VEE41 pKa = 4.81DD42 pKa = 3.83LFFSNKK48 pKa = 8.85PEE50 pKa = 4.27FYY52 pKa = 10.1RR53 pKa = 11.84FVFTEE58 pKa = 3.6QNNYY62 pKa = 9.57LRR64 pKa = 11.84EE65 pKa = 4.0KK66 pKa = 10.13LTDD69 pKa = 3.41KK70 pKa = 10.83VGRR73 pKa = 11.84AMDD76 pKa = 4.45LSDD79 pKa = 4.84EE80 pKa = 4.16IQRR83 pKa = 11.84DD84 pKa = 3.19KK85 pKa = 11.29DD86 pKa = 3.41AEE88 pKa = 4.19EE89 pKa = 4.01IEE91 pKa = 4.13KK92 pKa = 11.03DD93 pKa = 3.3KK94 pKa = 11.44EE95 pKa = 4.14AFLKK99 pKa = 10.04KK100 pKa = 10.16HH101 pKa = 6.21PGIDD105 pKa = 3.74FNEE108 pKa = 3.89LLEE111 pKa = 4.62FYY113 pKa = 10.85NEE115 pKa = 3.93EE116 pKa = 3.79VPNRR120 pKa = 11.84FKK122 pKa = 11.17KK123 pKa = 10.62QIDD126 pKa = 3.63KK127 pKa = 11.4LEE129 pKa = 4.21GVAFFEE135 pKa = 5.75AILDD139 pKa = 3.82YY140 pKa = 11.38FEE142 pKa = 4.71VLNSKK147 pKa = 10.43PEE149 pKa = 3.98EE150 pKa = 4.2GQKK153 pKa = 10.54EE154 pKa = 4.18EE155 pKa = 4.64EE156 pKa = 4.49KK157 pKa = 11.24NNLPKK162 pKa = 10.47EE163 pKa = 4.11VLGNGVSGVGYY174 pKa = 10.77ANNEE178 pKa = 4.31NIMTRR183 pKa = 11.84YY184 pKa = 9.53

Molecular weight:
21.64 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1S5RGR5|A0A1S5RGR5_9CAUD Portal protein OS=Helicobacter phage Pt1293U OX=1852669 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.32NINLIKK8 pKa = 10.35HH9 pKa = 5.56FRR11 pKa = 11.84NVEE14 pKa = 4.15NIKK17 pKa = 10.26KK18 pKa = 9.84RR19 pKa = 11.84HH20 pKa = 5.11LAFKK24 pKa = 10.13KK25 pKa = 9.42SKK27 pKa = 10.39KK28 pKa = 9.7RR29 pKa = 11.84VLEE32 pKa = 3.84FLKK35 pKa = 10.89NRR37 pKa = 11.84GYY39 pKa = 11.0RR40 pKa = 11.84DD41 pKa = 4.54FIAKK45 pKa = 9.48VKK47 pKa = 10.65SKK49 pKa = 10.48NQSDD53 pKa = 4.27DD54 pKa = 4.31EE55 pKa = 4.1ILEE58 pKa = 4.09NLEE61 pKa = 4.16LSYY64 pKa = 11.47LKK66 pKa = 10.42II67 pKa = 4.09

Molecular weight:
8.13 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

9542

43

880

265.1

30.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.487 ± 0.318

0.796 ± 0.142

5.02 ± 0.429

8.457 ± 0.423

4.758 ± 0.245

4.024 ± 0.238

1.572 ± 0.178

6.676 ± 0.247

11.109 ± 0.422

10.836 ± 0.431

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.897 ± 0.179

8.195 ± 0.434

2.389 ± 0.17

4.601 ± 0.318

3.836 ± 0.19

6.445 ± 0.213

4.674 ± 0.285

4.244 ± 0.337

0.472 ± 0.083

3.511 ± 0.15

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski