Zygotorulaspora mrakii (Zygosaccharomyces mrakii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi;

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5020 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7H9AXF5|A0A7H9AXF5_ZYGMR BSD domain-containing protein OS=Zygotorulaspora mrakii OX=42260 GN=HG535_0B01380 PE=4 SV=1
MM1 pKa = 7.96PEE3 pKa = 3.97LSYY6 pKa = 11.61EE7 pKa = 4.13EE8 pKa = 5.03IIDD11 pKa = 4.7HH12 pKa = 6.24IVSDD16 pKa = 4.02KK17 pKa = 10.72PLPNVVAVPDD27 pKa = 3.69VTLCEE32 pKa = 4.22SLRR35 pKa = 11.84TVSEE39 pKa = 4.0IPSRR43 pKa = 11.84PKK45 pKa = 9.55PWEE48 pKa = 3.48IANQSTYY55 pKa = 11.58SLDD58 pKa = 3.5GDD60 pKa = 4.32GNDD63 pKa = 4.11LATSIAFSQEE73 pKa = 3.19SSIQVQEE80 pKa = 4.08EE81 pKa = 4.15YY82 pKa = 11.05DD83 pKa = 3.68RR84 pKa = 11.84LNTDD88 pKa = 2.61VDD90 pKa = 3.45SSYY93 pKa = 11.8YY94 pKa = 10.51LGEE97 pKa = 4.4NEE99 pKa = 5.85LDD101 pKa = 3.68AQQ103 pKa = 4.08

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7H9B8E5|A0A7H9B8E5_ZYGMR 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Zygotorulaspora mrakii OX=42260 GN=HG535_0H02820 PE=3 SV=1
MM1 pKa = 7.18MMSLLYY7 pKa = 10.62KK8 pKa = 10.28PFIHH12 pKa = 6.12QNSRR16 pKa = 11.84RR17 pKa = 11.84TFTSLSSFSPLRR29 pKa = 11.84TLLPQNHH36 pKa = 5.88AQRR39 pKa = 11.84LIGLPHH45 pKa = 6.37NQLQSGQSILHH56 pKa = 5.8QISFPFGLNIQRR68 pKa = 11.84RR69 pKa = 11.84WKK71 pKa = 10.62SRR73 pKa = 11.84GNTFQPSTLKK83 pKa = 10.07RR84 pKa = 11.84KK85 pKa = 9.56RR86 pKa = 11.84RR87 pKa = 11.84IGFLARR93 pKa = 11.84ARR95 pKa = 11.84SKK97 pKa = 10.66QGSKK101 pKa = 9.78ILQRR105 pKa = 11.84RR106 pKa = 11.84KK107 pKa = 10.51AKK109 pKa = 9.78GRR111 pKa = 11.84WYY113 pKa = 9.08LTHH116 pKa = 7.23

Molecular weight: 13.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5020

0

5020

2512296

14

4919

500.5

56.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.787 ± 0.029

1.328 ± 0.012

5.87 ± 0.027

6.713 ± 0.036

4.422 ± 0.024

5.309 ± 0.031

2.165 ± 0.015

6.324 ± 0.022

7.017 ± 0.033

9.603 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.01

5.585 ± 0.028

4.183 ± 0.022

4.054 ± 0.029

4.699 ± 0.022

9.089 ± 0.045

5.559 ± 0.036

5.819 ± 0.022

1.047 ± 0.011

3.314 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski