Peptoniphilus harei ACS-146-V-Sch2b

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Peptoniphilus; Peptoniphilus harei

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1724 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4L0D6|E4L0D6_9FIRM Uncharacterized protein OS=Peptoniphilus harei ACS-146-V-Sch2b OX=908338 GN=HMPREF9286_0665 PE=4 SV=1
MM1 pKa = 7.4NNEE4 pKa = 4.18EE5 pKa = 4.56KK6 pKa = 10.58NLHH9 pKa = 5.46QHH11 pKa = 7.34DD12 pKa = 4.53EE13 pKa = 4.42DD14 pKa = 4.58CGCDD18 pKa = 3.41CGCGHH23 pKa = 6.67DD24 pKa = 4.75HH25 pKa = 5.81EE26 pKa = 7.11HH27 pKa = 6.76EE28 pKa = 5.35DD29 pKa = 4.16GYY31 pKa = 9.22QTITLTLEE39 pKa = 4.57DD40 pKa = 4.77DD41 pKa = 4.03SEE43 pKa = 4.64MVCIVLGIFEE53 pKa = 5.63CEE55 pKa = 3.89DD56 pKa = 3.18QEE58 pKa = 4.9YY59 pKa = 10.46IALLPEE65 pKa = 4.44DD66 pKa = 4.67EE67 pKa = 4.95EE68 pKa = 5.7DD69 pKa = 4.29GEE71 pKa = 5.04DD72 pKa = 3.67VFIYY76 pKa = 9.9KK77 pKa = 10.35YY78 pKa = 10.87KK79 pKa = 10.35EE80 pKa = 3.7INEE83 pKa = 4.33EE84 pKa = 4.08EE85 pKa = 4.44VEE87 pKa = 4.05LDD89 pKa = 3.61TIEE92 pKa = 4.8SEE94 pKa = 4.46EE95 pKa = 4.07EE96 pKa = 3.75FEE98 pKa = 4.55KK99 pKa = 10.93VSKK102 pKa = 9.74TFEE105 pKa = 4.34EE106 pKa = 4.33IFDD109 pKa = 4.94DD110 pKa = 4.25EE111 pKa = 5.16DD112 pKa = 3.75EE113 pKa = 4.33EE114 pKa = 4.58

Molecular weight: 13.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4KWZ8|E4KWZ8_9FIRM Putative membrane protein OS=Peptoniphilus harei ACS-146-V-Sch2b OX=908338 GN=HMPREF9286_1680 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.31NKK10 pKa = 8.92QRR12 pKa = 11.84KK13 pKa = 7.85RR14 pKa = 11.84EE15 pKa = 3.9HH16 pKa = 6.14GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84AVIKK32 pKa = 10.32ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.35GRR39 pKa = 11.84KK40 pKa = 8.86KK41 pKa = 10.58LSAA44 pKa = 3.95

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1724

0

1724

544872

30

4666

316.1

35.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.865 ± 0.068

0.777 ± 0.022

6.527 ± 0.056

8.063 ± 0.07

4.692 ± 0.05

6.49 ± 0.055

1.276 ± 0.022

9.055 ± 0.066

9.368 ± 0.073

9.426 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.513 ± 0.032

5.501 ± 0.056

2.786 ± 0.034

2.078 ± 0.027

3.898 ± 0.041

6.219 ± 0.044

4.587 ± 0.049

6.337 ± 0.053

0.532 ± 0.016

4.009 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski