Alligator sinensis (Chinese alligator)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Crocodylia;

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34942 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q0FWK3|A0A3Q0FWK3_ALLSI voltage-gated potassium channel subunit beta-3 OS=Alligator sinensis OX=38654 GN=KCNAB3 PE=3 SV=1
VV1 pKa = 6.39QEE3 pKa = 3.97VFEE6 pKa = 4.21YY7 pKa = 10.05VAVYY11 pKa = 6.69FTRR14 pKa = 11.84RR15 pKa = 11.84EE16 pKa = 3.97WEE18 pKa = 4.24LLDD21 pKa = 5.99DD22 pKa = 4.89DD23 pKa = 6.23DD24 pKa = 5.29KK25 pKa = 12.03VLYY28 pKa = 9.37QDD30 pKa = 4.76QMLKK34 pKa = 10.61NFNTLVSLGYY44 pKa = 10.07RR45 pKa = 11.84GPAPDD50 pKa = 5.75LICSIQQGQVEE61 pKa = 4.75LWVCDD66 pKa = 4.69DD67 pKa = 4.31EE68 pKa = 6.25DD69 pKa = 4.95CGEE72 pKa = 4.19ISGSDD77 pKa = 3.45DD78 pKa = 3.7LLAGEE83 pKa = 5.21LWLSSLLPSQQ93 pKa = 3.96

Molecular weight: 10.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q0HIE3|A0A3Q0HIE3_ALLSI DNA repair protein XRCC4 isoform X1 OS=Alligator sinensis OX=38654 GN=XRCC4 PE=4 SV=1
MM1 pKa = 7.76GYY3 pKa = 8.12LTARR7 pKa = 11.84ISHH10 pKa = 7.2CYY12 pKa = 9.59RR13 pKa = 11.84LQLGPDD19 pKa = 3.87GPGQCPVDD27 pKa = 4.0GGSDD31 pKa = 3.52TPAVASRR38 pKa = 11.84ARR40 pKa = 11.84VLRR43 pKa = 11.84VPQLSWSRR51 pKa = 11.84GSPAQGWEE59 pKa = 3.84GGLRR63 pKa = 11.84KK64 pKa = 8.42TDD66 pKa = 3.7TPSHH70 pKa = 6.13MGGSSIWEE78 pKa = 3.95AQGEE82 pKa = 4.57VLWIGWPALALGKK95 pKa = 10.14AAARR99 pKa = 11.84GEE101 pKa = 3.81WRR103 pKa = 11.84LGGKK107 pKa = 9.83RR108 pKa = 11.84GLPWGSANLGSSGQAGTQWLPPKK131 pKa = 10.06VRR133 pKa = 11.84PDD135 pKa = 3.66SVCATLQGWTTAALPMRR152 pKa = 11.84GCHH155 pKa = 6.36LARR158 pKa = 11.84AGRR161 pKa = 11.84WAQGAPGSRR170 pKa = 11.84LGLPACWKK178 pKa = 7.54QCKK181 pKa = 9.54RR182 pKa = 11.84GRR184 pKa = 11.84ARR186 pKa = 11.84AQLQCLSLVQGPSCSACLQKK206 pKa = 10.59PPQVLGTASVGSSQGRR222 pKa = 11.84AGWEE226 pKa = 4.23CPAQQHH232 pKa = 5.65SAVPVQTWAPAPGRR246 pKa = 11.84EE247 pKa = 4.21LQVGEE252 pKa = 3.71ARR254 pKa = 11.84ARR256 pKa = 11.84ARR258 pKa = 11.84AAGGHH263 pKa = 5.25SVGGSQWVDD272 pKa = 3.72GPWGLGRR279 pKa = 11.84QMPRR283 pKa = 11.84SIWTRR288 pKa = 11.84LALGCEE294 pKa = 4.4TPGPGQYY301 pKa = 9.63RR302 pKa = 11.84KK303 pKa = 9.96KK304 pKa = 10.86

Molecular weight: 32.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19111

15831

34942

23347094

31

33802

668.2

74.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.587 ± 0.012

2.15 ± 0.011

4.994 ± 0.011

7.351 ± 0.017

3.5 ± 0.01

6.008 ± 0.019

2.603 ± 0.006

4.692 ± 0.01

6.363 ± 0.021

9.48 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.278 ± 0.006

3.96 ± 0.01

5.771 ± 0.018

4.954 ± 0.013

5.254 ± 0.011

8.548 ± 0.016

5.547 ± 0.011

5.994 ± 0.01

1.14 ± 0.004

2.772 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski