Escherichia phage vB_EcoM-Ro157lw

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Wifcevirus; unclassified Wifcevirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A494RIE8|A0A494RIE8_9CAUD DNA-directed DNA polymerase OS=Escherichia phage vB_EcoM-Ro157lw OX=2144177 GN=vBEcoMRo157lw_00060 PE=4 SV=1
MM1 pKa = 6.99ITLSAIRR8 pKa = 11.84SARR11 pKa = 11.84VEE13 pKa = 3.86TSEE16 pKa = 3.71NSNVFGEE23 pKa = 4.48TLEE26 pKa = 4.13SVYY29 pKa = 10.73LSNVDD34 pKa = 4.69AIIEE38 pKa = 4.38SIYY41 pKa = 10.87EE42 pKa = 4.2PINFLDD48 pKa = 3.95LYY50 pKa = 10.42KK51 pKa = 10.92YY52 pKa = 10.09RR53 pKa = 11.84DD54 pKa = 3.77EE55 pKa = 6.5DD56 pKa = 3.54IVSYY60 pKa = 10.57RR61 pKa = 11.84VTINGFVYY69 pKa = 10.0TITEE73 pKa = 3.66IGGEE77 pKa = 4.28YY78 pKa = 9.71IAVPQLGGEE87 pKa = 4.03VTYY90 pKa = 9.65FKK92 pKa = 10.53IVSLL96 pKa = 4.08

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A494RD01|A0A494RD01_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM-Ro157lw OX=2144177 GN=vBEcoMRo157lw_00090 PE=4 SV=1
MM1 pKa = 6.8NTQGRR6 pKa = 11.84ITKK9 pKa = 8.15QRR11 pKa = 11.84RR12 pKa = 11.84ALQVLIHH19 pKa = 6.25YY20 pKa = 7.59LTRR23 pKa = 11.84LYY25 pKa = 8.6PTAHH29 pKa = 6.39EE30 pKa = 4.35QNPSTGHH37 pKa = 5.4LFKK40 pKa = 10.82RR41 pKa = 11.84RR42 pKa = 11.84NLSLKK47 pKa = 9.29TFALIQYY54 pKa = 9.76DD55 pKa = 3.74RR56 pKa = 11.84YY57 pKa = 11.08LDD59 pKa = 3.62GRR61 pKa = 11.84YY62 pKa = 9.78CGPKK66 pKa = 10.2DD67 pKa = 3.54SLL69 pKa = 3.8

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

20914

35

912

205.0

22.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.602 ± 0.33

0.975 ± 0.099

6.058 ± 0.198

6.728 ± 0.384

4.007 ± 0.141

7.359 ± 0.295

1.602 ± 0.137

6.503 ± 0.236

6.522 ± 0.369

7.392 ± 0.194

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.831 ± 0.157

5.049 ± 0.187

3.687 ± 0.144

3.887 ± 0.172

4.939 ± 0.198

6.23 ± 0.283

5.795 ± 0.306

6.986 ± 0.212

1.291 ± 0.089

3.557 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski