Herbidospora galbida

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Herbidospora

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U3MQV4|A0A4U3MQV4_9ACTN Cupin domain-containing protein OS=Herbidospora galbida OX=2575442 GN=FDA94_01810 PE=4 SV=1
MM1 pKa = 7.42SCGSVTAPDD10 pKa = 4.4PGAGAAPEE18 pKa = 4.36VTTPDD23 pKa = 3.95QIEE26 pKa = 4.3TDD28 pKa = 4.41LDD30 pKa = 4.15PPTDD34 pKa = 4.42PPASDD39 pKa = 4.32CDD41 pKa = 3.79VKK43 pKa = 11.49GEE45 pKa = 4.08NFSEE49 pKa = 4.21DD50 pKa = 3.18VRR52 pKa = 11.84LARR55 pKa = 11.84CLTEE59 pKa = 4.52EE60 pKa = 4.07FWTEE64 pKa = 3.52QFTASGGTYY73 pKa = 9.76QPITRR78 pKa = 11.84FVEE81 pKa = 4.05YY82 pKa = 10.73NGDD85 pKa = 4.04DD86 pKa = 4.01GPDD89 pKa = 3.37CGGQPSVPNNAFYY102 pKa = 11.09CPDD105 pKa = 3.12GHH107 pKa = 7.0FIAYY111 pKa = 8.31DD112 pKa = 3.63ATWLQGLYY120 pKa = 10.54DD121 pKa = 3.9QLGDD125 pKa = 3.44GAVYY129 pKa = 10.1VVIPHH134 pKa = 6.52EE135 pKa = 4.89FGHH138 pKa = 6.07SVQNQLMSNFEE149 pKa = 4.11FSVQAEE155 pKa = 4.22LQADD159 pKa = 4.99CYY161 pKa = 11.37AGGTLKK167 pKa = 11.06GLIDD171 pKa = 4.3GKK173 pKa = 10.56RR174 pKa = 11.84LQAQEE179 pKa = 4.33GDD181 pKa = 4.03DD182 pKa = 4.12AEE184 pKa = 5.18LMANLEE190 pKa = 4.25NAGDD194 pKa = 3.9PTDD197 pKa = 3.98AWWEE201 pKa = 4.06PGAHH205 pKa = 5.62GTAQQRR211 pKa = 11.84QLNFARR217 pKa = 11.84GLEE220 pKa = 4.29SGVGACC226 pKa = 5.52

Molecular weight: 24.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U3LNE7|A0A4U3LNE7_9ACTN KR domain-containing protein (Fragment) OS=Herbidospora galbida OX=2575442 GN=FDA94_38440 PE=4 SV=1
MM1 pKa = 7.48LRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84PRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84WSRR12 pKa = 11.84RR13 pKa = 11.84PLRR16 pKa = 11.84PPRR19 pKa = 11.84LLPHH23 pKa = 6.71RR24 pKa = 11.84LRR26 pKa = 11.84RR27 pKa = 11.84LRR29 pKa = 11.84LRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84LPRR36 pKa = 11.84LRR38 pKa = 11.84PRR40 pKa = 11.84RR41 pKa = 11.84CSPGPLPPLRR51 pKa = 11.84PPPLRR56 pKa = 11.84PSRR59 pKa = 11.84PRR61 pKa = 11.84PRR63 pKa = 11.84RR64 pKa = 11.84SGRR67 pKa = 11.84RR68 pKa = 11.84PVV70 pKa = 2.89

Molecular weight: 8.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7514

0

7514

2491651

18

4260

331.6

35.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.943 ± 0.037

0.742 ± 0.008

5.983 ± 0.022

5.561 ± 0.024

2.994 ± 0.016

9.249 ± 0.027

2.179 ± 0.013

3.74 ± 0.017

2.185 ± 0.022

10.447 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.844 ± 0.012

2.002 ± 0.022

5.996 ± 0.024

2.601 ± 0.015

7.846 ± 0.034

4.988 ± 0.023

6.055 ± 0.031

8.861 ± 0.027

1.634 ± 0.01

2.15 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski