Magnaporthe oryzae polymycovirus 1

Taxonomy: Viruses; Riboviria; Polymycoviridae; Polymycovirus

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410TEN7|A0A410TEN7_9VIRU Uncharacterized protein OS=Magnaporthe oryzae polymycovirus 1 OX=2509266 GN=ORF2a PE=2 SV=1
MM1 pKa = 7.93ADD3 pKa = 3.6LTRR6 pKa = 11.84LRR8 pKa = 11.84SILLDD13 pKa = 3.47TATPAAQIGMALYY26 pKa = 10.92HH27 pKa = 6.42MFPAPDD33 pKa = 3.46ASALLDD39 pKa = 3.78SQSVRR44 pKa = 11.84QAVLWLSSAPVALYY58 pKa = 10.75GGVVGAAGAQPSDD71 pKa = 3.68FARR74 pKa = 11.84LLDD77 pKa = 4.21KK78 pKa = 11.31LDD80 pKa = 4.53DD81 pKa = 3.83STAARR86 pKa = 11.84AATIAHH92 pKa = 6.2AMVARR97 pKa = 11.84DD98 pKa = 4.08RR99 pKa = 11.84EE100 pKa = 3.84WDD102 pKa = 3.42RR103 pKa = 11.84AVRR106 pKa = 11.84DD107 pKa = 4.51GYY109 pKa = 8.06QTQVSVHH116 pKa = 6.11GPGSARR122 pKa = 11.84VDD124 pKa = 3.17AQTSVEE130 pKa = 4.34FAALDD135 pKa = 3.74ALTDD139 pKa = 3.5AGYY142 pKa = 11.05AGIDD146 pKa = 3.51DD147 pKa = 4.44AVAAFADD154 pKa = 3.92LRR156 pKa = 11.84RR157 pKa = 11.84VVAKK161 pKa = 6.94STCVIRR167 pKa = 11.84STQLARR173 pKa = 11.84SRR175 pKa = 11.84HH176 pKa = 4.42AVIARR181 pKa = 11.84FPLSDD186 pKa = 3.21GTVRR190 pKa = 11.84LVFTPWCLSRR200 pKa = 11.84RR201 pKa = 11.84SALAVAGLLVSDD213 pKa = 3.74VRR215 pKa = 11.84RR216 pKa = 11.84GVLHH220 pKa = 6.62DD221 pKa = 3.8RR222 pKa = 11.84VVKK225 pKa = 10.47RR226 pKa = 11.84ALTVTTFAARR236 pKa = 11.84TSLQAVRR243 pKa = 11.84DD244 pKa = 3.85LTGPALALLTRR255 pKa = 11.84YY256 pKa = 10.09AYY258 pKa = 10.53DD259 pKa = 3.81PVRR262 pKa = 11.84MALVGDD268 pKa = 4.73DD269 pKa = 3.94GVEE272 pKa = 3.94QQTVGSRR279 pKa = 11.84LPHH282 pKa = 4.56VTVAFAAIFSSGSADD297 pKa = 3.55PLIALARR304 pKa = 11.84ARR306 pKa = 11.84FEE308 pKa = 4.41LANGLPNLNMNASARR323 pKa = 11.84QYY325 pKa = 10.88FEE327 pKa = 4.26YY328 pKa = 11.12AEE330 pKa = 4.8DD331 pKa = 4.61PYY333 pKa = 11.66SDD335 pKa = 3.66VVLFSAALAQARR347 pKa = 11.84QQRR350 pKa = 11.84EE351 pKa = 4.05KK352 pKa = 10.74SEE354 pKa = 3.82HH355 pKa = 3.92GHH357 pKa = 5.68RR358 pKa = 11.84VNKK361 pKa = 10.05GAMLGYY367 pKa = 9.22VARR370 pKa = 11.84GRR372 pKa = 11.84TALDD376 pKa = 3.78RR377 pKa = 11.84NTSACVNRR385 pKa = 11.84IEE387 pKa = 4.32EE388 pKa = 4.47TVVTLRR394 pKa = 11.84ARR396 pKa = 11.84GHH398 pKa = 5.94EE399 pKa = 3.97PSSYY403 pKa = 10.73IMVVEE408 pKa = 4.1WGGAINIATVLASAAVAGIDD428 pKa = 3.73VALDD432 pKa = 3.57VADD435 pKa = 4.75SGIDD439 pKa = 3.51LPGADD444 pKa = 4.22IYY446 pKa = 11.63GDD448 pKa = 3.54NDD450 pKa = 3.88DD451 pKa = 4.92PAHH454 pKa = 6.97HH455 pKa = 6.2YY456 pKa = 10.13QLYY459 pKa = 9.47LARR462 pKa = 11.84ASSMNLPRR470 pKa = 11.84MPTVEE475 pKa = 4.19YY476 pKa = 10.25TKK478 pKa = 10.1GTPLVVKK485 pKa = 10.55LEE487 pKa = 4.43SVLSAVKK494 pKa = 10.22AAGDD498 pKa = 3.25KK499 pKa = 10.61RR500 pKa = 11.84LVYY503 pKa = 10.06VHH505 pKa = 6.69GGIARR510 pKa = 11.84EE511 pKa = 3.9RR512 pKa = 11.84QVPISTAIDD521 pKa = 3.45CNNRR525 pKa = 11.84LDD527 pKa = 4.16ALSHH531 pKa = 6.22VIADD535 pKa = 4.65PPPLLYY541 pKa = 10.25TSEE544 pKa = 4.0ILLPPFCVHH553 pKa = 7.22SYY555 pKa = 10.27QCTAEE560 pKa = 4.32ALLEE564 pKa = 4.21TGGLVDD570 pKa = 5.0DD571 pKa = 4.95TCDD574 pKa = 3.17QCEE577 pKa = 4.17LVSRR581 pKa = 11.84ACAVLSSILDD591 pKa = 3.43RR592 pKa = 11.84PGIRR596 pKa = 11.84LVKK599 pKa = 9.74ARR601 pKa = 11.84SMYY604 pKa = 10.53AHH606 pKa = 6.86NSHH609 pKa = 6.88FAVEE613 pKa = 4.16VFPGEE618 pKa = 3.92VDD620 pKa = 3.88LSHH623 pKa = 7.3DD624 pKa = 3.69TATTTDD630 pKa = 2.9ACVAANVLRR639 pKa = 11.84NHH641 pKa = 7.45TYY643 pKa = 7.92NTPPPEE649 pKa = 5.29GDD651 pKa = 2.97ASKK654 pKa = 10.99DD655 pKa = 3.19ITSPEE660 pKa = 4.05LMEE663 pKa = 3.98LTRR666 pKa = 11.84PIIEE670 pKa = 4.24MVSAAMSGGPVAKK683 pKa = 10.49VSAEE687 pKa = 3.98RR688 pKa = 11.84ADD690 pKa = 3.55QVAASIAA697 pKa = 3.4

Molecular weight: 74.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410TEM9|A0A410TEM9_9VIRU Uncharacterized protein OS=Magnaporthe oryzae polymycovirus 1 OX=2509266 GN=ORF3a PE=2 SV=1
MM1 pKa = 7.52SLNDD5 pKa = 3.69SVSAATAKK13 pKa = 10.7SLTQHH18 pKa = 5.72SPEE21 pKa = 4.08VFTTFARR28 pKa = 11.84LVTLGFSPSEE38 pKa = 3.58LRR40 pKa = 11.84AALVRR45 pKa = 11.84VANGEE50 pKa = 4.15EE51 pKa = 4.36VPLPGNLGRR60 pKa = 11.84PRR62 pKa = 11.84PLTINAWSFCVDD74 pKa = 3.27RR75 pKa = 11.84GQYY78 pKa = 10.8ADD80 pKa = 3.95TYY82 pKa = 11.33GLDD85 pKa = 3.5DD86 pKa = 4.45SEE88 pKa = 4.79AGRR91 pKa = 11.84LKK93 pKa = 10.94GVLRR97 pKa = 11.84ANADD101 pKa = 3.59DD102 pKa = 4.47GVAEE106 pKa = 4.12ITKK109 pKa = 10.53LVTDD113 pKa = 4.4KK114 pKa = 11.35LRR116 pKa = 11.84ISGSARR122 pKa = 11.84AVHH125 pKa = 5.01VTKK128 pKa = 10.64SGMPQSVVTGSGLKK142 pKa = 9.78PGKK145 pKa = 10.1QGGADD150 pKa = 3.79LKK152 pKa = 10.22TVILTNPALFGMYY165 pKa = 9.47TFVPSDD171 pKa = 3.46SGVPGPLRR179 pKa = 11.84YY180 pKa = 9.31RR181 pKa = 11.84CRR183 pKa = 11.84IGSGLYY189 pKa = 10.43ACFPSKK195 pKa = 10.47KK196 pKa = 10.0GAIDD200 pKa = 3.2VARR203 pKa = 11.84ICRR206 pKa = 11.84VHH208 pKa = 5.87GRR210 pKa = 11.84NHH212 pKa = 5.35EE213 pKa = 4.56AIAGRR218 pKa = 11.84VYY220 pKa = 10.45FIRR223 pKa = 11.84EE224 pKa = 3.71GKK226 pKa = 10.55APLTGADD233 pKa = 3.12IPEE236 pKa = 4.15KK237 pKa = 11.01VKK239 pKa = 10.83FDD241 pKa = 3.4GKK243 pKa = 10.49LRR245 pKa = 11.84PDD247 pKa = 3.73EE248 pKa = 4.67PVPADD253 pKa = 3.64PVNSPTTEE261 pKa = 3.92VNN263 pKa = 3.08

Molecular weight: 27.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2338

263

766

584.5

62.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.19 ± 1.199

0.941 ± 0.212

6.373 ± 0.289

4.063 ± 0.369

2.866 ± 0.36

7.613 ± 0.776

2.524 ± 0.173

3.678 ± 0.058

2.823 ± 0.518

9.196 ± 0.472

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.139 ± 0.203

2.395 ± 0.182

6.373 ± 0.559

2.31 ± 0.217

7.742 ± 0.238

7.699 ± 0.153

6.33 ± 0.166

9.324 ± 0.811

0.642 ± 0.059

2.78 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski