Aureobasidium pullulans EXF-150

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Dothideales; Saccotheciaceae; Aureobasidium; Aureobasidium pullulans

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A074XYG5|A0A074XYG5_AURPU Uncharacterized protein OS=Aureobasidium pullulans EXF-150 OX=1043002 GN=M438DRAFT_283088 PE=4 SV=1
MM1 pKa = 7.3HH2 pKa = 7.68LSTFSLLALSAALANAKK19 pKa = 8.94TDD21 pKa = 3.72LDD23 pKa = 3.82GCTRR27 pKa = 11.84TDD29 pKa = 2.81ISSPAGASYY38 pKa = 11.03AWIVPGTGEE47 pKa = 4.31LCDD50 pKa = 4.58PLDD53 pKa = 4.44CGGGRR58 pKa = 11.84APPKK62 pKa = 8.5TTVPGCAAYY71 pKa = 10.57VGTEE75 pKa = 4.29TYY77 pKa = 10.48SPSYY81 pKa = 10.17LAGSTAPATAAASASAVVTSAEE103 pKa = 3.98VTAAASATATPTAGASDD120 pKa = 4.57DD121 pKa = 4.21NSDD124 pKa = 5.94DD125 pKa = 4.34EE126 pKa = 6.55DD127 pKa = 5.04DD128 pKa = 5.29SNDD131 pKa = 3.59DD132 pKa = 3.8TSDD135 pKa = 3.88DD136 pKa = 4.02SSDD139 pKa = 4.6DD140 pKa = 3.82EE141 pKa = 5.89DD142 pKa = 4.42PLGLAGIDD150 pKa = 3.26AAQEE154 pKa = 3.89STYY157 pKa = 10.36VWRR160 pKa = 11.84TADD163 pKa = 3.54NPTATFNPFTASAFPSDD180 pKa = 4.9VSTFWIPVDD189 pKa = 4.48LGSTTSWEE197 pKa = 4.08AAVPTVLSGGYY208 pKa = 8.27TDD210 pKa = 4.7IVPASMPASSDD221 pKa = 3.35LPAICAIATNSGCGIDD237 pKa = 4.14MSTMTISRR245 pKa = 11.84SQTSTTGSPTSVGTASTSGLSRR267 pKa = 11.84SWVKK271 pKa = 9.65TIPTYY276 pKa = 10.76TRR278 pKa = 11.84VAANDD283 pKa = 3.12AMVRR287 pKa = 11.84AGVSGQSSTSSTASGSASRR306 pKa = 11.84SASASTEE313 pKa = 4.01TSTGAAGKK321 pKa = 9.79VGCAVGGVLAAAMGALIVLL340 pKa = 4.62

Molecular weight: 33.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A074Y258|A0A074Y258_AURPU Uncharacterized protein OS=Aureobasidium pullulans EXF-150 OX=1043002 GN=M438DRAFT_394009 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TNNTIRR36 pKa = 11.84YY37 pKa = 5.73NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11844

0

11844

5256702

49

4975

443.8

49.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.752 ± 0.022

1.281 ± 0.009

5.774 ± 0.018

6.03 ± 0.026

3.752 ± 0.014

6.535 ± 0.023

2.454 ± 0.012

4.929 ± 0.018

4.99 ± 0.021

8.788 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.01

3.822 ± 0.013

5.821 ± 0.027

4.18 ± 0.018

5.814 ± 0.022

8.359 ± 0.026

6.143 ± 0.017

6.043 ± 0.018

1.455 ± 0.009

2.804 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski