Lactobacillus sakei subsp. sakei (strain 23K)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Latilactobacillus; Latilactobacillus sakei; Latilactobacillus sakei subsp. sakei

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1872 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q38VG6|Q38VG6_LACSS Putative priming glycosyl transferase OS=Lactobacillus sakei subsp. sakei (strain 23K) OX=314315 GN=LCA_1510_g PE=3 SV=1
MM1 pKa = 7.11ATAKK5 pKa = 10.11VVYY8 pKa = 10.71ASMTGNNEE16 pKa = 3.75EE17 pKa = 3.89IADD20 pKa = 3.77IVEE23 pKa = 4.14EE24 pKa = 4.05ALEE27 pKa = 4.11NLDD30 pKa = 3.57VSVEE34 pKa = 4.08TSEE37 pKa = 5.29ISQADD42 pKa = 3.48PSDD45 pKa = 4.64FEE47 pKa = 4.76DD48 pKa = 3.42TDD50 pKa = 3.42ICIVCSYY57 pKa = 10.64TYY59 pKa = 11.11GDD61 pKa = 5.25DD62 pKa = 4.25GDD64 pKa = 5.07LPDD67 pKa = 4.27EE68 pKa = 4.6AVDD71 pKa = 4.29FYY73 pKa = 11.82EE74 pKa = 5.25DD75 pKa = 3.89LKK77 pKa = 11.71EE78 pKa = 3.78MDD80 pKa = 4.04LTGKK84 pKa = 10.55VYY86 pKa = 9.98GVCGSGDD93 pKa = 3.21TFYY96 pKa = 11.46DD97 pKa = 3.86EE98 pKa = 4.35FCKK101 pKa = 10.99VVDD104 pKa = 4.09DD105 pKa = 4.13FAGVFEE111 pKa = 4.32QTGATKK117 pKa = 10.58GSDD120 pKa = 3.49VVKK123 pKa = 10.93VDD125 pKa = 4.52LAPEE129 pKa = 4.46AEE131 pKa = 4.85DD132 pKa = 3.99IEE134 pKa = 4.44HH135 pKa = 6.77LEE137 pKa = 4.11KK138 pKa = 10.67FVAEE142 pKa = 4.39IVAKK146 pKa = 10.61QSALL150 pKa = 3.48

Molecular weight: 16.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q38UF6|Q38UF6_LACSS Uncharacterized protein OS=Lactobacillus sakei subsp. sakei (strain 23K) OX=314315 GN=LCA_1873 PE=4 SV=1
MM1 pKa = 5.72TTKK4 pKa = 9.85RR5 pKa = 11.84TFQPKK10 pKa = 9.03KK11 pKa = 7.46RR12 pKa = 11.84HH13 pKa = 5.51KK14 pKa = 9.79EE15 pKa = 3.74RR16 pKa = 11.84VHH18 pKa = 8.0GFMKK22 pKa = 10.62RR23 pKa = 11.84MNTKK27 pKa = 10.02NGRR30 pKa = 11.84KK31 pKa = 8.78VLARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.94GRR41 pKa = 11.84KK42 pKa = 8.67VLSAA46 pKa = 4.05

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1872

0

1872

542104

8

1987

289.6

32.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.783 ± 0.064

0.487 ± 0.014

5.439 ± 0.056

5.554 ± 0.068

4.119 ± 0.039

6.625 ± 0.055

2.011 ± 0.026

7.179 ± 0.053

5.867 ± 0.047

10.201 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.754 ± 0.029

4.35 ± 0.045

3.739 ± 0.03

5.478 ± 0.059

3.915 ± 0.047

5.415 ± 0.048

6.595 ± 0.063

7.103 ± 0.05

0.975 ± 0.021

3.412 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski