Hubei virga-like virus 17

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJW0|A0A1L3KJW0_9VIRU Uncharacterized protein OS=Hubei virga-like virus 17 OX=1923332 PE=4 SV=1
MM1 pKa = 7.6LKK3 pKa = 10.52FILAEE8 pKa = 3.93FEE10 pKa = 4.12NFAKK14 pKa = 10.86VKK16 pKa = 8.87FTMFRR21 pKa = 11.84LVLILIIEE29 pKa = 4.37VVVVNCKK36 pKa = 9.67NYY38 pKa = 9.32RR39 pKa = 11.84QEE41 pKa = 4.09VVINNVSLVKK51 pKa = 10.49LDD53 pKa = 4.21LVPLFAFEE61 pKa = 4.38TGVPVYY67 pKa = 10.63FKK69 pKa = 11.21DD70 pKa = 5.38DD71 pKa = 4.0LDD73 pKa = 4.28LKK75 pKa = 10.54NLLVMRR81 pKa = 11.84EE82 pKa = 3.9LDD84 pKa = 3.62YY85 pKa = 11.63LKK87 pKa = 11.03NFYY90 pKa = 10.64TEE92 pKa = 3.78MNNLYY97 pKa = 10.34RR98 pKa = 11.84YY99 pKa = 9.87FEE101 pKa = 4.25YY102 pKa = 10.84NYY104 pKa = 10.18VRR106 pKa = 11.84VKK108 pKa = 9.46LHH110 pKa = 6.6CLDD113 pKa = 4.3ALSEE117 pKa = 4.11NLIQDD122 pKa = 3.79YY123 pKa = 10.82DD124 pKa = 4.11CEE126 pKa = 4.49LDD128 pKa = 4.02YY129 pKa = 11.51SCEE132 pKa = 3.52PHH134 pKa = 7.37IRR136 pKa = 11.84IKK138 pKa = 10.54VAHH141 pKa = 5.74TWYY144 pKa = 10.62GSAVYY149 pKa = 10.35ACLGLNRR156 pKa = 11.84TRR158 pKa = 11.84VFNSTNVLVRR168 pKa = 11.84VHH170 pKa = 6.74KK171 pKa = 10.54LAFPGVGIYY180 pKa = 8.51MKK182 pKa = 10.29NVSDD186 pKa = 4.43IYY188 pKa = 11.57GNLDD192 pKa = 3.66DD193 pKa = 5.72SGLRR197 pKa = 11.84YY198 pKa = 10.15DD199 pKa = 5.08DD200 pKa = 5.69LVLADD205 pKa = 3.93YY206 pKa = 10.81EE207 pKa = 4.19IDD209 pKa = 3.56GVRR212 pKa = 11.84KK213 pKa = 9.69FIFTYY218 pKa = 10.33KK219 pKa = 10.82YY220 pKa = 9.94MLNQSSAAFTDD231 pKa = 4.3AYY233 pKa = 10.35RR234 pKa = 11.84IYY236 pKa = 10.97GDD238 pKa = 3.32WDD240 pKa = 3.66NVCVEE245 pKa = 4.23NKK247 pKa = 9.32VSKK250 pKa = 10.46FMYY253 pKa = 10.24YY254 pKa = 10.03YY255 pKa = 10.21FGYY258 pKa = 8.56IAKK261 pKa = 10.59YY262 pKa = 9.94NGTDD266 pKa = 2.94KK267 pKa = 11.03VIPHH271 pKa = 6.59MPLMVYY277 pKa = 9.89VGRR280 pKa = 11.84RR281 pKa = 11.84NAAADD286 pKa = 3.61YY287 pKa = 10.3ASEE290 pKa = 4.01FFTYY294 pKa = 8.36YY295 pKa = 10.33QCTEE299 pKa = 4.09EE300 pKa = 4.25NLKK303 pKa = 10.74EE304 pKa = 3.9PLLVNVGGLDD314 pKa = 3.35FLKK317 pKa = 10.81YY318 pKa = 10.57NKK320 pKa = 9.33PNCTDD325 pKa = 3.57YY326 pKa = 11.74VKK328 pKa = 10.88VSSDD332 pKa = 3.22EE333 pKa = 4.14FQCNVIFDD341 pKa = 4.63LGNPFLKK348 pKa = 10.61AICPDD353 pKa = 3.64DD354 pKa = 4.86YY355 pKa = 11.04IVKK358 pKa = 10.26KK359 pKa = 10.86DD360 pKa = 3.77VVDD363 pKa = 3.83HH364 pKa = 6.53NKK366 pKa = 10.07NDD368 pKa = 3.26FDD370 pKa = 4.91TEE372 pKa = 4.48VNCIKK377 pKa = 10.49VRR379 pKa = 11.84TIKK382 pKa = 10.68KK383 pKa = 9.0SWFSEE388 pKa = 3.87LFVFLEE394 pKa = 4.12KK395 pKa = 10.51KK396 pKa = 10.06VLHH399 pKa = 5.89LVEE402 pKa = 5.67GIIKK406 pKa = 10.18EE407 pKa = 4.0IAQVLSEE414 pKa = 4.31SVKK417 pKa = 10.26VLVDD421 pKa = 3.46EE422 pKa = 4.41LVKK425 pKa = 10.68VLKK428 pKa = 11.02DD429 pKa = 3.06MGPIFKK435 pKa = 9.96PLLEE439 pKa = 6.0DD440 pKa = 2.85IFKK443 pKa = 10.52DD444 pKa = 3.38VKK446 pKa = 10.67EE447 pKa = 3.88IAKK450 pKa = 10.14EE451 pKa = 3.98VFKK454 pKa = 11.32DD455 pKa = 4.01FGQIFKK461 pKa = 10.77DD462 pKa = 3.32IFADD466 pKa = 3.96AVEE469 pKa = 4.61LLDD472 pKa = 5.11DD473 pKa = 4.81LVNFTLGFVFGIIRR487 pKa = 11.84YY488 pKa = 8.54LLNLLVQLEE497 pKa = 3.81KK498 pKa = 10.62DD499 pKa = 3.8YY500 pKa = 11.6YY501 pKa = 10.09VFEE504 pKa = 4.24ILVAYY509 pKa = 9.57VVLRR513 pKa = 11.84ILLVHH518 pKa = 6.27NLSVVVILVIVCLTFGVEE536 pKa = 3.57RR537 pKa = 11.84RR538 pKa = 11.84YY539 pKa = 11.26DD540 pKa = 3.65SVVYY544 pKa = 9.63DD545 pKa = 3.36IWSNVTIDD553 pKa = 3.73MKK555 pKa = 11.24LLNN558 pKa = 4.37

Molecular weight: 65.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJW0|A0A1L3KJW0_9VIRU Uncharacterized protein OS=Hubei virga-like virus 17 OX=1923332 PE=4 SV=1
MM1 pKa = 7.5EE2 pKa = 5.3AQPQQMQGNGRR13 pKa = 11.84TGWRR17 pKa = 11.84VEE19 pKa = 4.14NKK21 pKa = 10.11VGDD24 pKa = 4.08LDD26 pKa = 4.76IISDD30 pKa = 3.61VLGAYY35 pKa = 8.81KK36 pKa = 10.28RR37 pKa = 11.84LPMYY41 pKa = 7.7PFPMFVLMLTLIVGLAEE58 pKa = 4.28IYY60 pKa = 10.84NGGEE64 pKa = 3.85KK65 pKa = 10.04PLEE68 pKa = 3.87YY69 pKa = 10.54LLNGIQADD77 pKa = 4.23LNNEE81 pKa = 4.47NIPTWEE87 pKa = 3.9KK88 pKa = 9.69TALKK92 pKa = 8.9ICKK95 pKa = 9.26YY96 pKa = 9.25VLEE99 pKa = 5.14FIIAHH104 pKa = 6.66KK105 pKa = 10.36IKK107 pKa = 10.91VFGFLMISIPVIIRR121 pKa = 11.84PSKK124 pKa = 8.12NNVYY128 pKa = 9.93IWVLLVAILIMMRR141 pKa = 11.84NWGFFEE147 pKa = 4.76YY148 pKa = 10.22IVIGNLFYY156 pKa = 10.42IYY158 pKa = 9.72TQLLSLTNKK167 pKa = 9.76FLIAMLIVVVVLWNFVLPVKK187 pKa = 10.37SNKK190 pKa = 9.28IPPGAPSPPPVVSGPAPPSRR210 pKa = 11.84KK211 pKa = 7.22TT212 pKa = 3.24

Molecular weight: 23.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3009

212

2239

1003.0

114.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.018 ± 0.378

2.127 ± 0.443

7.511 ± 1.488

5.716 ± 0.394

5.45 ± 0.526

5.218 ± 0.608

1.961 ± 0.49

5.849 ± 1.417

8.109 ± 0.588

9.837 ± 1.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.891 ± 0.452

5.018 ± 0.719

3.091 ± 1.418

2.193 ± 0.309

4.021 ± 0.442

6.181 ± 1.323

4.021 ± 0.465

9.837 ± 1.113

0.931 ± 0.375

5.018 ± 0.687
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski