Klebsiella phage ST16-OXA48phi5.3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482ILW3|A0A482ILW3_9CAUD Uncharacterized protein OS=Klebsiella phage ST16-OXA48phi5.3 OX=2510482 PE=4 SV=1
MM1 pKa = 7.6IAPIFAVCAASQAVRR16 pKa = 11.84DD17 pKa = 4.25LLGSTPVRR25 pKa = 11.84LYY27 pKa = 10.48PFGMQDD33 pKa = 3.56DD34 pKa = 4.84NIVYY38 pKa = 9.06PYY40 pKa = 11.02AVWQNVGGFPEE51 pKa = 4.72NYY53 pKa = 9.94LNQRR57 pKa = 11.84PDD59 pKa = 3.33ADD61 pKa = 3.91HH62 pKa = 6.96YY63 pKa = 11.15SLQVDD68 pKa = 4.27VYY70 pKa = 10.79GDD72 pKa = 3.44TDD74 pKa = 3.61TDD76 pKa = 3.97VIAVARR82 pKa = 11.84ALRR85 pKa = 11.84DD86 pKa = 4.0AIEE89 pKa = 4.31GKK91 pKa = 10.57AYY93 pKa = 7.31ITRR96 pKa = 11.84WGEE99 pKa = 3.6QSRR102 pKa = 11.84DD103 pKa = 3.8PEE105 pKa = 4.36TMRR108 pKa = 11.84YY109 pKa = 9.44RR110 pKa = 11.84YY111 pKa = 10.46SFDD114 pKa = 3.35VDD116 pKa = 4.19WITTRR121 pKa = 3.64

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482IIU0|A0A482IIU0_9CAUD Uncharacterized protein OS=Klebsiella phage ST16-OXA48phi5.3 OX=2510482 PE=4 SV=1
MM1 pKa = 7.7IDD3 pKa = 3.41NRR5 pKa = 11.84TVSAIDD11 pKa = 3.79LALQKK16 pKa = 10.72HH17 pKa = 5.26PTPVGDD23 pKa = 4.01LFAAIRR29 pKa = 11.84HH30 pKa = 5.09GRR32 pKa = 11.84MKK34 pKa = 10.47RR35 pKa = 11.84CFSRR39 pKa = 11.84DD40 pKa = 2.83TAIRR44 pKa = 11.84YY45 pKa = 7.74LAFFMTSRR53 pKa = 11.84AFGRR57 pKa = 11.84SGFKK61 pKa = 9.87QRR63 pKa = 11.84YY64 pKa = 7.55PDD66 pKa = 3.42VQVIHH71 pKa = 6.88PLNPEE76 pKa = 3.9LSSWQRR82 pKa = 11.84GAVTLEE88 pKa = 3.81YY89 pKa = 10.66FNAHH93 pKa = 4.55QRR95 pKa = 11.84TVRR98 pKa = 11.84RR99 pKa = 11.84LRR101 pKa = 11.84RR102 pKa = 11.84ILARR106 pKa = 11.84KK107 pKa = 9.79RR108 pKa = 11.84EE109 pKa = 3.97MQKK112 pKa = 9.29WCEE115 pKa = 3.94KK116 pKa = 8.46WDD118 pKa = 3.72AMHH121 pKa = 7.29DD122 pKa = 3.64RR123 pKa = 11.84YY124 pKa = 11.06VKK126 pKa = 10.27EE127 pKa = 4.1RR128 pKa = 11.84EE129 pKa = 3.83EE130 pKa = 4.08LQASKK135 pKa = 9.97PAEE138 pKa = 3.83VRR140 pKa = 11.84NASQHH145 pKa = 4.66AA146 pKa = 3.53

Molecular weight: 17.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

8567

39

815

168.0

18.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.77 ± 0.567

1.062 ± 0.227

5.673 ± 0.269

6.397 ± 0.307

3.84 ± 0.199

7.074 ± 0.392

1.903 ± 0.239

5.918 ± 0.263

5.86 ± 0.343

9.046 ± 0.418

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.801 ± 0.193

4.004 ± 0.194

4.226 ± 0.357

4.155 ± 0.329

6.292 ± 0.52

6.397 ± 0.286

5.404 ± 0.246

6.28 ± 0.211

1.354 ± 0.147

2.545 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski